BacMap

Definition	Mesorhizobium sp. BNC1, complete genome.
Accession	NC_008254
Length	4,412,446

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The map label for this gene is braF [H]

Identifier: 110632837

GI number: 110632837

Start: 525781

End: 526557

Strand: Reverse

Name: braF [H]

Synonym: Meso_0476

Alternate gene names: 110632837

Gene position: 526557-525781 (Counterclockwise)

Preceding gene: 110632838

Following gene: 110632836

Centisome position: 11.93

GC content: 56.5

Gene sequence:

>777_bases
ATGACTGATCCAGTTCTAAACGTATCCAACCTGACGGTACGCTTCGGCGGGCTGGTTGCGGTCGACAGCGTCTCCGTTGC
CCTCAAGGCTGGCAAGGTGCACGGTCTCATTGGACCGAACGGAGCGGGCAAGACGACCTTCTTCAACGCGATCACCGGAC
TGGTGCCGCAGTCCGACGGCAGGGTCCAATATCTAGGGAAAGACATCACCAATCTGCCTGCCCATCGGCGAGCGGATATT
GGCATCCGACGTTCGTTCCAGTCAGTTCAATTAGTACAGAGTCTTACGGTACTTGAAAACATTCTTATCGGTCTGCACAG
TGGATCGGCAGGCAAGGGCTGGCGTGGCATCGGTGCACTCTTCGGCTTTGAAGGCCCGGATCGGGCCGAGCAGGAGCAAG
TTTGGGACGTTGCAGCCTTCCTCGGCATCGAACATACCCTGTTTAAGTCAATCGCCGAGTTGACATTCGCTGAGCAGCGG
TTCGTGGAAATCGCCCGAGCCATCGTCTCCCGCCCGAAAGTGCTGATGCTGGACGAGCCGGCTGCTGGGCTTAGTGAGGC
TGAGATTGAAAAGCTGGATCGGCTGCTAAGGCGACTCGCCCTTGAATGGGGCATGGCTATTCTGCTGGTGGAACACGTGC
TTTCCCTGGTTCTCGTTGTCTCAGATGAAATTACGGTTTTAGACAACGGCAGGTTCGTCATCCATGGGCTGCCGGACGAG
GTAGCGCGCGATCCGCGTGTCAAATCCGCCTATCTCGGAGAAGAAGTACATGCTTAA

Upstream 100 bases:

>100_bases
CCGCGTGAAATGCTCGTGCGTGGCATCGTGCGCGAAAGGCGTCCGGTTGCTACACCCGGACGGGATCAGGACCTGGAACC
TATTGGTGTGGGAGTCACCC

Downstream 100 bases:

>100_bases
GGTCGAGGGGATTTCTGTCGGATACGGTGCGATCGAGGTTCTGCGTGACCTGAGTTTCACCGTTCCGAAAGGAAAGGTCG
TCGCGCTGCTGGGAGGTAAT

Product: ABC transporter related

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTDPVLNVSNLTVRFGGLVAVDSVSVALKAGKVHGLIGPNGAGKTTFFNAITGLVPQSDGRVQYLGKDITNLPAHRRADI
GIRRSFQSVQLVQSLTVLENILIGLHSGSAGKGWRGIGALFGFEGPDRAEQEQVWDVAAFLGIEHTLFKSIAELTFAEQR
FVEIARAIVSRPKVLMLDEPAAGLSEAEIEKLDRLLRRLALEWGMAILLVEHVLSLVLVVSDEITVLDNGRFVIHGLPDE
VARDPRVKSAYLGEEVHA

Sequences:

>Translated_258_residues
MTDPVLNVSNLTVRFGGLVAVDSVSVALKAGKVHGLIGPNGAGKTTFFNAITGLVPQSDGRVQYLGKDITNLPAHRRADI
GIRRSFQSVQLVQSLTVLENILIGLHSGSAGKGWRGIGALFGFEGPDRAEQEQVWDVAAFLGIEHTLFKSIAELTFAEQR
FVEIARAIVSRPKVLMLDEPAAGLSEAEIEKLDRLLRRLALEWGMAILLVEHVLSLVLVVSDEITVLDNGRFVIHGLPDE
VARDPRVKSAYLGEEVHA
>Mature_257_residues
TDPVLNVSNLTVRFGGLVAVDSVSVALKAGKVHGLIGPNGAGKTTFFNAITGLVPQSDGRVQYLGKDITNLPAHRRADIG
IRRSFQSVQLVQSLTVLENILIGLHSGSAGKGWRGIGALFGFEGPDRAEQEQVWDVAAFLGIEHTLFKSIAELTFAEQRF
VEIARAIVSRPKVLMLDEPAAGLSEAEIEKLDRLLRRLALEWGMAILLVEHVLSLVLVVSDEITVLDNGRFVIHGLPDEV
ARDPRVKSAYLGEEVHA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI21536376, Length=224, Percent_Identity=30.8035714285714, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI105990541, Length=241, Percent_Identity=29.045643153527, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI27881501, Length=228, Percent_Identity=27.1929824561404, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI30795238, Length=228, Percent_Identity=27.1929824561404, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI11967969, Length=230, Percent_Identity=27.8260869565217, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI31657092, Length=230, Percent_Identity=25.2173913043478, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI171184400, Length=237, Percent_Identity=26.5822784810127, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=38.1322957198444, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789593, Length=253, Percent_Identity=31.6205533596838, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48995001, Length=229, Percent_Identity=32.3144104803493, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787712, Length=241, Percent_Identity=32.3651452282158, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=27.8225806451613, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787792, Length=241, Percent_Identity=28.2157676348548, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081709, Length=252, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788210, Length=232, Percent_Identity=30.1724137931034, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788761, Length=252, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1789991, Length=234, Percent_Identity=29.4871794871795, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788472, Length=229, Percent_Identity=27.0742358078603, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787370, Length=243, Percent_Identity=27.1604938271605, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082268, Length=260, Percent_Identity=29.6153846153846, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081791, Length=255, Percent_Identity=26.6666666666667, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1790190, Length=233, Percent_Identity=27.8969957081545, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788897, Length=233, Percent_Identity=30.0429184549356, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081782, Length=263, Percent_Identity=26.2357414448669, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786563, Length=240, Percent_Identity=29.5833333333333, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1786398, Length=256, Percent_Identity=28.515625, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786253, Length=229, Percent_Identity=26.6375545851528, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790467, Length=250, Percent_Identity=27.2, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786345, Length=252, Percent_Identity=25.3968253968254, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789672, Length=261, Percent_Identity=23.3716475095785, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI48994997, Length=252, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790544, Length=248, Percent_Identity=27.0161290322581, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788450, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994943, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787029, Length=246, Percent_Identity=24.7967479674797, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788225, Length=251, Percent_Identity=28.2868525896414, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789032, Length=232, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786703, Length=214, Percent_Identity=30.8411214953271, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081834, Length=241, Percent_Identity=26.9709543568465, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790739, Length=255, Percent_Identity=27.0588235294118, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788506, Length=227, Percent_Identity=28.1938325991189, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789873, Length=227, Percent_Identity=29.5154185022026, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=260, Percent_Identity=24.2307692307692, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789963, Length=260, Percent_Identity=30.3846153846154, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788002, Length=235, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788644, Length=265, Percent_Identity=27.1698113207547, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786803, Length=255, Percent_Identity=24.3137254901961, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082267, Length=211, Percent_Identity=27.0142180094787, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786975, Length=219, Percent_Identity=27.3972602739726, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=220, Percent_Identity=25.9090909090909, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=220, Percent_Identity=25.9090909090909, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115534520, Length=237, Percent_Identity=24.8945147679325, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=211, Percent_Identity=29.8578199052133, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221512771, Length=227, Percent_Identity=28.6343612334802, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28574744, Length=215, Percent_Identity=25.1162790697674, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643674, Length=231, Percent_Identity=25.5411255411255, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666092, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574533, Length=203, Percent_Identity=26.1083743842365, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85724864, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580555, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85816269, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580553, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27973; Mature: 27841

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPVLNVSNLTVRFGGLVAVDSVSVALKAGKVHGLIGPNGAGKTTFFNAITGLVPQSDG
CCCCCCCCCCEEEEECCEEEECHHHEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
RVQYLGKDITNLPAHRRADIGIRRSFQSVQLVQSLTVLENILIGLHSGSAGKGWRGIGAL
CEEECCCCHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FGFEGPDRAEQEQVWDVAAFLGIEHTLFKSIAELTFAEQRFVEIARAIVSRPKVLMLDEP
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AAGLSEAEIEKLDRLLRRLALEWGMAILLVEHVLSLVLVVSDEITVLDNGRFVIHGLPDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHH
VARDPRVKSAYLGEEVHA
HHCCCCCHHHHCCHHCCC
>Mature Secondary Structure 
TDPVLNVSNLTVRFGGLVAVDSVSVALKAGKVHGLIGPNGAGKTTFFNAITGLVPQSDG
CCCCCCCCCEEEEECCEEEECHHHEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
RVQYLGKDITNLPAHRRADIGIRRSFQSVQLVQSLTVLENILIGLHSGSAGKGWRGIGAL
CEEECCCCHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FGFEGPDRAEQEQVWDVAAFLGIEHTLFKSIAELTFAEQRFVEIARAIVSRPKVLMLDEP
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AAGLSEAEIEKLDRLLRRLALEWGMAILLVEHVLSLVLVVSDEITVLDNGRFVIHGLPDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHH
VARDPRVKSAYLGEEVHA
HHCCCCCHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]