Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 110632838

GI number: 110632838

Start: 526550

End: 527590

Strand: Reverse

Name: livM [H]

Synonym: Meso_0477

Alternate gene names: 110632838

Gene position: 527590-526550 (Counterclockwise)

Preceding gene: 110632839

Following gene: 110632837

Centisome position: 11.96

GC content: 56.87

Gene sequence:

>1041_bases
ATGGATCGAACCGCTATTATGAAGAAGCCCCTGATTTATGGCGGATTGGCCGCAGCTCTTCTTGCGGTCCCGTTGCTGAC
CAACGGCTACACGCAGTATGTTGTCAATCTCGTGACCGTCTATGTTGTGCTTGGAGTCGGCCTTAACATACTGCTTGGCT
ATGCTGGGCAGTTCGCCTTCGCCAACGCTGCGTTCATGGGCATCGGCGCCTATATAACTGCGATATTGTCCAGTCAGCTC
GGCTTGCCGTTCTGGGTGTGCATTCCAGCCGCAGGCGTCGTGACCGCATTGATTGGGACAGCTTGCGCCGTGCCGGCAAT
GCGGATGGCCAGCATCTACCTTGCACTCGTTACCTTCGCTTTCGCCGAGCTAATGGTGTGGGTCTTCATCAACTGGAAGG
GTGTAACGAACGGATCGAACGGCATTGGCGTCAGCGCTCCGGATCTTTTCGGCTATGTGTTTCGCGGCGACCAACGCCTA
TATTATCTTCTTCTGCCGTGCTGCCTGACAATGCTTTGGCTCGCATCGCGTATCCTGAAGTCGTACATAGGCCGGTCATT
CGTCATCATACGCGAAAGCGAGATCGTAGCCCGCTGCAACGGGATCGATGTCAGCCGCACGAAGATGATTGCCTTCGGCC
TCTCGGCATTCTTCGGTGGCGTAGGTGGAGCGCTGTTCGCCTATACGGTCGGCCTTGTGCTGCCGCAGTCGTTCGGACTT
TTCCAGCTGGTAATTCACATAGCAATTGTGGTGCTAGGCGGCATGGCCAGTCTTGCTGGCGCCATCATCGGGGCACTGCT
CCTGACTACGCTTCCAGAATTCTTGCGCGACTTCCAGAGTCTGCAGGAGGTGATGTTCGGAACGGCGCTCGTAATCGTAG
CGATATTCATGCCCCGGGGTATTGCCGGCGCGTTGACTCGGTTCGGCATTCTCCCGCGTGAAATGCTCGTGCGTGGCATC
GTGCGCGAAAGGCGTCCGGTTGCTACACCCGGACGGGATCAGGACCTGGAACCTATTGGTGTGGGAGTCACCCATGACTG
A

Upstream 100 bases:

>100_bases
CAACGGCGTTCCAGCAGGTATCTCCGTTCCTCATCATTATTATCGTCTTGTTCGTCCGTCCTCACGGCCTGTTTGGACGC
CGACCGGTGGAGCGAGTTTG

Downstream 100 bases:

>100_bases
TCCAGTTCTAAACGTATCCAACCTGACGGTACGCTTCGGCGGGCTGGTTGCGGTCGACAGCGTCTCCGTTGCCCTCAAGG
CTGGCAAGGTGCACGGTCTC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDRTAIMKKPLIYGGLAAALLAVPLLTNGYTQYVVNLVTVYVVLGVGLNILLGYAGQFAFANAAFMGIGAYITAILSSQL
GLPFWVCIPAAGVVTALIGTACAVPAMRMASIYLALVTFAFAELMVWVFINWKGVTNGSNGIGVSAPDLFGYVFRGDQRL
YYLLLPCCLTMLWLASRILKSYIGRSFVIIRESEIVARCNGIDVSRTKMIAFGLSAFFGGVGGALFAYTVGLVLPQSFGL
FQLVIHIAIVVLGGMASLAGAIIGALLLTTLPEFLRDFQSLQEVMFGTALVIVAIFMPRGIAGALTRFGILPREMLVRGI
VRERRPVATPGRDQDLEPIGVGVTHD

Sequences:

>Translated_346_residues
MDRTAIMKKPLIYGGLAAALLAVPLLTNGYTQYVVNLVTVYVVLGVGLNILLGYAGQFAFANAAFMGIGAYITAILSSQL
GLPFWVCIPAAGVVTALIGTACAVPAMRMASIYLALVTFAFAELMVWVFINWKGVTNGSNGIGVSAPDLFGYVFRGDQRL
YYLLLPCCLTMLWLASRILKSYIGRSFVIIRESEIVARCNGIDVSRTKMIAFGLSAFFGGVGGALFAYTVGLVLPQSFGL
FQLVIHIAIVVLGGMASLAGAIIGALLLTTLPEFLRDFQSLQEVMFGTALVIVAIFMPRGIAGALTRFGILPREMLVRGI
VRERRPVATPGRDQDLEPIGVGVTHD
>Mature_346_residues
MDRTAIMKKPLIYGGLAAALLAVPLLTNGYTQYVVNLVTVYVVLGVGLNILLGYAGQFAFANAAFMGIGAYITAILSSQL
GLPFWVCIPAAGVVTALIGTACAVPAMRMASIYLALVTFAFAELMVWVFINWKGVTNGSNGIGVSAPDLFGYVFRGDQRL
YYLLLPCCLTMLWLASRILKSYIGRSFVIIRESEIVARCNGIDVSRTKMIAFGLSAFFGGVGGALFAYTVGLVLPQSFGL
FQLVIHIAIVVLGGMASLAGAIIGALLLTTLPEFLRDFQSLQEVMFGTALVIVAIFMPRGIAGALTRFGILPREMLVRGI
VRERRPVATPGRDQDLEPIGVGVTHD

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=302, Percent_Identity=34.1059602649007, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37001; Mature: 37001

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTAIMKKPLIYGGLAAALLAVPLLTNGYTQYVVNLVTVYVVLGVGLNILLGYAGQFAF
CCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ANAAFMGIGAYITAILSSQLGLPFWVCIPAAGVVTALIGTACAVPAMRMASIYLALVTFA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAELMVWVFINWKGVTNGSNGIGVSAPDLFGYVFRGDQRLYYLLLPCCLTMLWLASRILK
HHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SYIGRSFVIIRESEIVARCNGIDVSRTKMIAFGLSAFFGGVGGALFAYTVGLVLPQSFGL
HHCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
FQLVIHIAIVVLGGMASLAGAIIGALLLTTLPEFLRDFQSLQEVMFGTALVIVAIFMPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IAGALTRFGILPREMLVRGIVRERRPVATPGRDQDLEPIGVGVTHD
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRTAIMKKPLIYGGLAAALLAVPLLTNGYTQYVVNLVTVYVVLGVGLNILLGYAGQFAF
CCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ANAAFMGIGAYITAILSSQLGLPFWVCIPAAGVVTALIGTACAVPAMRMASIYLALVTFA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAELMVWVFINWKGVTNGSNGIGVSAPDLFGYVFRGDQRLYYLLLPCCLTMLWLASRILK
HHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SYIGRSFVIIRESEIVARCNGIDVSRTKMIAFGLSAFFGGVGGALFAYTVGLVLPQSFGL
HHCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
FQLVIHIAIVVLGGMASLAGAIIGALLLTTLPEFLRDFQSLQEVMFGTALVIVAIFMPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IAGALTRFGILPREMLVRGIVRERRPVATPGRDQDLEPIGVGVTHD
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]