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The map label for this gene is mscL [H]

Identifier: 110632451

GI number: 110632451

Start: 118021

End: 118449

Strand: Reverse

Name: mscL [H]

Synonym: Meso_0089

Alternate gene names: 110632451

Gene position: 118449-118021 (Counterclockwise)

Preceding gene: 110632453

Following gene: 110632446

Centisome position: 2.68

GC content: 58.51

Gene sequence:

>429_bases
ATGGCAAACGGAATGCTGAATGAATTCAGGGAGTTCATAGCCCGAGGCAATGTCATAGACCTGGCCGTGGGCGTGATTAT
CGGCGGCGCATTTGGCGGCATCGTCAGCTCTCTGACCGACGACATCATCATGCCCATCGTGGGCGCGGTCTTCGGCGGGT
TCGACTTCTCCAATTATTTTGTCCCGCTCGCTTCCGGTGTGACAGCGGATACGCTTGCCCAGGCGCGTGAGCAGGGGGCA
GTGCTGGCCTACGGCAATTTCATCACCGTTCTTCTGAACTTCCTCATCCTCGCCTGGATCATTTTCCTGATGGTCAGGGC
CGTGAACAGGCTGCGCAGGCGGCAAGCAGAGCAGCCGGCTGAGCCCGCGGCACCGCCTCAGGATGTCCAACTCCTGACGG
AAATTCGGGATCTGCTGGCAAGAAGATAA

Upstream 100 bases:

>100_bases
GCGCAGGCACAACAAAGATTCCCTGTTCGCTCACATTTGCTATTCTGATAAGCGCTGGGTGTGGGTCACCCGGATTCTCA
ACCGTGCCAGGGGGACTGAA

Downstream 100 bases:

>100_bases
GCGAAAGGCGGCCACAGCCGCCATGGAGAGAAAAGAAGGCAGGCAGTGAGGCGGCACCTGATCCGCCTCACCAATCCCGT
TCTATCCGCCTGATTTCGAG

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MANGMLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYFVPLASGVTADTLAQAREQGA
VLAYGNFITVLLNFLILAWIIFLMVRAVNRLRRRQAEQPAEPAAPPQDVQLLTEIRDLLARR

Sequences:

>Translated_142_residues
MANGMLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYFVPLASGVTADTLAQAREQGA
VLAYGNFITVLLNFLILAWIIFLMVRAVNRLRRRQAEQPAEPAAPPQDVQLLTEIRDLLARR
>Mature_141_residues
ANGMLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYFVPLASGVTADTLAQAREQGAV
LAYGNFITVLLNFLILAWIIFLMVRAVNRLRRRQAEQPAEPAAPPQDVQLLTEIRDLLARR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=139, Percent_Identity=48.9208633093525, Blast_Score=123, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15421; Mature: 15290

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGMLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VPLASGVTADTLAQAREQGAVLAYGNFITVLLNFLILAWIIFLMVRAVNRLRRRQAEQPA
HHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPAAPPQDVQLLTEIRDLLARR
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANGMLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VPLASGVTADTLAQAREQGAVLAYGNFITVLLNFLILAWIIFLMVRAVNRLRRRQAEQPA
HHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPAAPPQDVQLLTEIRDLLARR
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA