Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 110632424

GI number: 110632424

Start: 84734

End: 86626

Strand: Reverse

Name: proP [H]

Synonym: Meso_0062

Alternate gene names: 110632424

Gene position: 86626-84734 (Counterclockwise)

Preceding gene: 110632425

Following gene: 110632413

Centisome position: 1.96

GC content: 60.96

Gene sequence:

>1893_bases
ATGACAGTTGCATCGACGGTCGATACCGGCCAGCGCGGCATGACGCGCGAGGAAAAGAAGGTCATCTTCGCCTCCTCGCT
CGGCACGGTATTCGAGTGGTATGATTTTTATCTCTATGGGTCGCTCGCCGTCTTCATCGGCGCTACCTTCTTCAGCCAAT
ATCCCGAGGCTACGCGGAACATCTTTGCGCTGCTGGCCTTTGCAGCGGGCTTCCTGGTGCGGCCCTTCGGGGCCCTGGTT
TTCGGCCGCATCGGCGATCTCGTGGGACGAAAATACACGTTCCTGATCACTATTGTGATCATGGGTCTTTCAACCTTTCT
CGTGGGCCTGCTGCCCGGCTCCGCCTCGATCGGCATTGCCGCGCCAATCATCCTTATTGCGCTACGCATGCTGCAAGGCC
TGGCTCTCGGCGGCGAATATGGCGGCGCAGCCACCTATGTCGCCGAGCACGCGCCGGATAACAGGCGCGGCTATTACACG
TCGTGGATCCAGACCACGGCGACACTCGGCCTCTTTCTTTCGCTCATCATAATCCTGCTGGTGCAAGCCTCCATGGGTCG
AGAAGCTTTCACCGCCTGGGGCTGGCGCATACCTTTCCTGCTCTCCTTCATCCTGCTCGCGATATCAGTCTGGATCCGGC
TCTCACTCAACGAATCGCCGACTTTCCAGCGCATGAAGGAAGAAGGCAAAGGCTCCAAAGCGCCCCTGTCGGAGGCCTTC
GGCCAGTGGAAGAACGCCAAGATAGCGCTCCTGGCGCTGCTCGGCCTGACGGCGGGCCAGGCAGTGGTTTGGTACAATGG
CCAGTTCTACGCGCTCTTCTTCCTGCAGAACGTGCTCAAGGTCGACGCCCAGTCCGTCAACATCATGATCGCCCTTTCGC
TGGCGCTCGCCACGCCCTTCTTCATTTTCTTCGGCTGGCTGTCGGACAAAGTGGGCCGCAAGGTGATCATCATGGCCGGG
CTGGCGCTCGCCATCGTCACCTATTTTCCGCTGTTCAAGGCGCTCACCTGGGCCGCTAATCCCGCTCTTGCCCAAGCTCA
GGCGCAGGTGCGGGCTACTGTCACCGCCGCTCCGGGCGACTGCAAGTTCCAGTTCAACCCGACTGGCACGGCGAAATTCA
CGACCTCCTGCGACATCGCCACTTCGTTCCTGACACGTACTTCCGTGCCGTATGACGTGGCGCTGACCGCGCCAGCGGGC
ACACCAGCAACGGTGCAGATCGGCAATGCAACAACCATCGAGTCCTATGACGCCGTCGCGGCAGGCGCCGACGCGCGGGC
AAGGGAGTCTGCCTTCGCACATTCCGTCAACCTTGCGCTGCAACAGGCAGGGTACCCGTTGGTTCGCGATCCCGCCAGGG
TAGCAGAGCCGAAGCTCGACGCCTTCATTGCTGCCAATCCGGAGCTTAACCTCGATGCAGCGGCCATCCGAGGCAGCCAG
GGCGCTACGGTGCCGGCGGAGCAGGCGGTGGAAGATGGGTTGCTGACGGCCGCGGAAGCCGCGGCAGCCGGCAATGAGGT
GGCCGTTTACACCATTCCCGGTGCAGGTGCGTTCAAAATGGTGGCAAATCCGGAGGCGGTGAACTGGCCGCTGGTCATCG
GCATCCTGTTCATACTGGTCGTCTATGTGACCATGGTCTACGGCCCGATCGCCGCGATCCTGGTGGAAATGTTCCCGACC
CGCATCCGCTACACCGGCATGTCATTGCCATACCACATCGGCAATGGCTGGTTTGGCGGGCTGCTGCCGGCAACGGTTTT
CGCCATGAGTGCCTATCGGGGCGATATCTACTTCGGCCTCTGGTATCCGGTCGCCGTGGCCGCGATGTCGCTCATTATCG
GCACCTTGTTCATACGGGACACGCTAGGCACCAATCTAAATACCAAGGAGTAG

Upstream 100 bases:

>100_bases
AAAGTCTCGCTGCATTTGCGGCTCCGATTCTGGCATTTCTTAAGGTGAACGGTGCGCGCTGAGGAGATGCCCGCGTCGTT
TTCATACCTGGGAGGACACT

Downstream 100 bases:

>100_bases
CCCCTTCTCCTCCCCTGCCCTGGCCCGGCGCTTCGCGCCGGGTCTTTTTTAGCAGCCACAACCGAAGGCGGCGCCAAAGA
CTCCTAGTTAGCCTCGCCCG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTVASTVDTGQRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAVFIGATFFSQYPEATRNIFALLAFAAGFLVRPFGALV
FGRIGDLVGRKYTFLITIVIMGLSTFLVGLLPGSASIGIAAPIILIALRMLQGLALGGEYGGAATYVAEHAPDNRRGYYT
SWIQTTATLGLFLSLIIILLVQASMGREAFTAWGWRIPFLLSFILLAISVWIRLSLNESPTFQRMKEEGKGSKAPLSEAF
GQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIALSLALATPFFIFFGWLSDKVGRKVIIMAG
LALAIVTYFPLFKALTWAANPALAQAQAQVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRTSVPYDVALTAPAG
TPATVQIGNATTIESYDAVAAGADARARESAFAHSVNLALQQAGYPLVRDPARVAEPKLDAFIAANPELNLDAAAIRGSQ
GATVPAEQAVEDGLLTAAEAAAAGNEVAVYTIPGAGAFKMVANPEAVNWPLVIGILFILVVYVTMVYGPIAAILVEMFPT
RIRYTGMSLPYHIGNGWFGGLLPATVFAMSAYRGDIYFGLWYPVAVAAMSLIIGTLFIRDTLGTNLNTKE

Sequences:

>Translated_630_residues
MTVASTVDTGQRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAVFIGATFFSQYPEATRNIFALLAFAAGFLVRPFGALV
FGRIGDLVGRKYTFLITIVIMGLSTFLVGLLPGSASIGIAAPIILIALRMLQGLALGGEYGGAATYVAEHAPDNRRGYYT
SWIQTTATLGLFLSLIIILLVQASMGREAFTAWGWRIPFLLSFILLAISVWIRLSLNESPTFQRMKEEGKGSKAPLSEAF
GQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIALSLALATPFFIFFGWLSDKVGRKVIIMAG
LALAIVTYFPLFKALTWAANPALAQAQAQVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRTSVPYDVALTAPAG
TPATVQIGNATTIESYDAVAAGADARARESAFAHSVNLALQQAGYPLVRDPARVAEPKLDAFIAANPELNLDAAAIRGSQ
GATVPAEQAVEDGLLTAAEAAAAGNEVAVYTIPGAGAFKMVANPEAVNWPLVIGILFILVVYVTMVYGPIAAILVEMFPT
RIRYTGMSLPYHIGNGWFGGLLPATVFAMSAYRGDIYFGLWYPVAVAAMSLIIGTLFIRDTLGTNLNTKE
>Mature_629_residues
TVASTVDTGQRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAVFIGATFFSQYPEATRNIFALLAFAAGFLVRPFGALVF
GRIGDLVGRKYTFLITIVIMGLSTFLVGLLPGSASIGIAAPIILIALRMLQGLALGGEYGGAATYVAEHAPDNRRGYYTS
WIQTTATLGLFLSLIIILLVQASMGREAFTAWGWRIPFLLSFILLAISVWIRLSLNESPTFQRMKEEGKGSKAPLSEAFG
QWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIALSLALATPFFIFFGWLSDKVGRKVIIMAGL
ALAIVTYFPLFKALTWAANPALAQAQAQVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRTSVPYDVALTAPAGT
PATVQIGNATTIESYDAVAAGADARARESAFAHSVNLALQQAGYPLVRDPARVAEPKLDAFIAANPELNLDAAAIRGSQG
ATVPAEQAVEDGLLTAAEAAAAGNEVAVYTIPGAGAFKMVANPEAVNWPLVIGILFILVVYVTMVYGPIAAILVEMFPTR
IRYTGMSLPYHIGNGWFGGLLPATVFAMSAYRGDIYFGLWYPVAVAAMSLIIGTLFIRDTLGTNLNTKE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=327, Percent_Identity=36.3914373088685, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788292, Length=339, Percent_Identity=33.0383480825959, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=327, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=235, Percent_Identity=42.1276595744681, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=25.5520504731861, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 67508; Mature: 67377

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVASTVDTGQRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAVFIGATFFSQYPEATRN
CCCCCCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFALLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLITIVIMGLSTFLVGLLPGSASIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
APIILIALRMLQGLALGGEYGGAATYVAEHAPDNRRGYYTSWIQTTATLGLFLSLIIILL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VQASMGREAFTAWGWRIPFLLSFILLAISVWIRLSLNESPTFQRMKEEGKGSKAPLSEAF
HHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCHHHHH
GQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIALSLALATPF
CCCCCCHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
FIFFGWLSDKVGRKVIIMAGLALAIVTYFPLFKALTWAANPALAQAQAQVRATVTAAPGD
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEECCCC
CKFQFNPTGTAKFTTSCDIATSFLTRTSVPYDVALTAPAGTPATVQIGNATTIESYDAVA
EEEEECCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEECCCCHHH
AGADARARESAFAHSVNLALQQAGYPLVRDPARVAEPKLDAFIAANPELNLDAAAIRGSQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCCCCCCCHHHEECCC
GATVPAEQAVEDGLLTAAEAAAAGNEVAVYTIPGAGAFKMVANPEAVNWPLVIGILFILV
CCCCCHHHHHHHCHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHH
VYVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPATVFAMSAYRGDIYFGL
HHHHHHHHHHHHHHHHHCCHHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEH
WYPVAVAAMSLIIGTLFIRDTLGTNLNTKE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVASTVDTGQRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAVFIGATFFSQYPEATRN
CCCCCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFALLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLITIVIMGLSTFLVGLLPGSASIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
APIILIALRMLQGLALGGEYGGAATYVAEHAPDNRRGYYTSWIQTTATLGLFLSLIIILL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VQASMGREAFTAWGWRIPFLLSFILLAISVWIRLSLNESPTFQRMKEEGKGSKAPLSEAF
HHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCHHHHH
GQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIALSLALATPF
CCCCCCHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
FIFFGWLSDKVGRKVIIMAGLALAIVTYFPLFKALTWAANPALAQAQAQVRATVTAAPGD
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEECCCC
CKFQFNPTGTAKFTTSCDIATSFLTRTSVPYDVALTAPAGTPATVQIGNATTIESYDAVA
EEEEECCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEECCCCHHH
AGADARARESAFAHSVNLALQQAGYPLVRDPARVAEPKLDAFIAANPELNLDAAAIRGSQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCCCCCCCHHHEECCC
GATVPAEQAVEDGLLTAAEAAAAGNEVAVYTIPGAGAFKMVANPEAVNWPLVIGILFILV
CCCCCHHHHHHHCHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHH
VYVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPATVFAMSAYRGDIYFGL
HHHHHHHHHHHHHHHHHCCHHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEH
WYPVAVAAMSLIIGTLFIRDTLGTNLNTKE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]