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The map label for this gene is bioB fragment [H]

Identifier: 109948248

GI number: 109948248

Start: 1550246

End: 1550518

Strand: Reverse

Name: bioB fragment [H]

Synonym: Hac_1785

Alternate gene names: 109948248

Gene position: 1550518-1550246 (Counterclockwise)

Preceding gene: 109948249

Following gene: 109948247

Centisome position: 99.78

GC content: 38.83

Gene sequence:

>273_bases
ATGGAATTGACAAAGATGGAAATATCAAATTTTAGATCAATCAAAGATTTAGAAATTAAAGCCAAAGAATTTTTGCCTAA
CGCTAGGCTTATGGCAGCTGGGGGGCGTGAAGTGGTGTTTAAAGATAACGATAAAAAGGAAGCCAAGCTTTTTGAATACG
GCATCAACGCAGTGGTGTTAGGGGACTATTTAACCACCAAAGGCAAAGCCCCTAAAAAAGATATAGAAAGACTGCTCTCT
TATGGCTTGAAAATGGCGGCAAGCTGTCATTAA

Upstream 100 bases:

>100_bases
AAAAGAAATGCAAGATTTAGCGAAAGCCTTGTTTGACGCTTTGCCTTGCGAGCACCAATATTTGTTTGAGGATTGTTTGA
AGCGCTAAAAGCGAGCAGCA

Downstream 100 bases:

>100_bases
TGAGAGAACTTTTTAAAAGCATCAGAGGGTTTTTGTGCCTTCTTAAAATGATTTTCCCCCAGCGCTTGAAAAACGCCTTT
TGGGGGTTAAGCGAGTTGTT

Product: biotin synthetase fragment

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MELTKMEISNFRSIKDLEIKAKEFLPNARLMAAGGREVVFKDNDKKEAKLFEYGINAVVLGDYLTTKGKAPKKDIERLLS
YGLKMAASCH

Sequences:

>Translated_90_residues
MELTKMEISNFRSIKDLEIKAKEFLPNARLMAAGGREVVFKDNDKKEAKLFEYGINAVVLGDYLTTKGKAPKKDIERLLS
YGLKMAASCH
>Mature_90_residues
MELTKMEISNFRSIKDLEIKAKEFLPNARLMAAGGREVVFKDNDKKEAKLFEYGINAVVLGDYLTTKGKAPKKDIERLLS
YGLKMAASCH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 10139; Mature: 10139

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTKMEISNFRSIKDLEIKAKEFLPNARLMAAGGREVVFKDNDKKEAKLFEYGINAVVL
CCCCCCCHHCCCCCCCCEEEHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHCCCEEEE
GDYLTTKGKAPKKDIERLLSYGLKMAASCH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELTKMEISNFRSIKDLEIKAKEFLPNARLMAAGGREVVFKDNDKKEAKLFEYGINAVVL
CCCCCCCHHCCCCCCCCEEEHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHCCCEEEE
GDYLTTKGKAPKKDIERLLSYGLKMAASCH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA