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The map label for this gene is dnaG [H]

Identifier: 109948169

GI number: 109948169

Start: 1476836

End: 1478515

Strand: Reverse

Name: dnaG [H]

Synonym: Hac_1696

Alternate gene names: 109948169

Gene position: 1478515-1476836 (Counterclockwise)

Preceding gene: 109948176

Following gene: 109948168

Centisome position: 95.15

GC content: 37.2

Gene sequence:

>1680_bases
ATGATCCTTAAAAGTTCCGTTGATCGCCTTTTACAAGTTGCAGATATTGTAGAAGTCATTAGTTCTTATGTGGATTTAAG
AAAAACGGGTTCTAATTACATGGCTTGTTGCCCTTTCCATGATGAAAGGAGTGCGAGTTTTAGCGTCAATCAAGCTAAAG
GGTTTTACCATTGTTTTGGGTGTGGGGCGAGTGGGGATAGCATTCAATTTGTGATGGAGTTTGAAAAGCTTTCGTTTGTG
GAAGCGCTTGAGAAACTAGCCCACCGATTCAATGTGACTTTAGAATATGATAAAGGCGTTTATTACGATTATAAAGAAGA
TTACCACCTTTTAGAAATGGTGAGCTCGTTGTATCAAGAAGAGCTTTTTAACGCCCCCTTTTTTTTAGATTATTTGCAAA
AAAGAGGGCTTAATATAGAGAGTATCAGAGCGTTTAAATTAGGGTTATGCGTAAATAGAATTTATCATGGCATTGAAAAT
AAAGGCTTGAATAAGGACAAGCTCATTGAATTAGGTGTGTTAGGAAAAAACGATAACAATCAGAAAACCTATTTACGCTT
TTTAGATCGCATTATGTTCCCTATTTATAACCCTAGCGCTCAAGTGGTGGGTTTTGGAGGGCGCACCTTAAAAGAAAAAG
GAGCCAAATATATCAATTCGCCCCAAAGCAAGCTTTTTGATAAATCCAGTTTGCTTTATGGCTATCATTTGGCTAAAGAA
AGCATCTATAAACAAAAACAAGTCATTGTAACAGAGGGGTATTTAGATGTGATTTTATTGCACCAAGCGGGTTTTAAAAA
TGCCATAGCCACGCTTGGGACGGCCTTAACACCATCGCATTTGCCCTTGCTTAAAAAAGGCGAGCCAGAGATTTTTTTGA
GCTATGATGGGGATAAAGCAGGGAGGAATGCGGCGTATAAAGCGAGCTTGATGTTGGCTAAAGAGCAAAGAAAAGGGGGA
GTGATCTTATTTGAAAACAACCTAGACCCAGCGGATATGATCGCTAATAACCAGATTGAAACCTTAAAAAATTGGTTATC
GCACCCCATAGCTTTTATTGAATTTGTTTTAAGGCATATGGCGAGTTCTTATGTTTTGGATGACCCTTTAGAAAAAGATA
AGGCTCTTAAAGAAATGTTAGGGTTTTTGAAAAACTTTTCCTTGCTTTTACAAAGCGAATACAAGCCCTTAATCGCTACG
CTTTTACAAGTGCCTTCGCATGTTTTAGGGATCAAAGAGCGCTCATCTTTCCAACCTTTTTATTCAAAAACAGAAAAATC
TAATTATTCTCAAAAGTTTGTGCATGTTTCTAACACGCTCAGTTTGGAATTTTTAGAAAAATTGGTGATCCGCTATCTTT
TAGAAGACAGGAGTTTATTAGATTTGGCGGTGGGTTATATCCATAGTGGGGTATTCTTGCATAAAAAACAAGAATTTGAC
GCTTTGTGTCAAGAAAAATTAGATGACCCTAAATTAGTTGCGTTATTATTAGATGCGAATTTACCCCTAAAAAAAGGGGG
GTTTGAAAAGGAATTGCGTTTGTTGATTTTACGCTATTTTGAACGGCAACTCAAAGAAATCCCTAAAAGCCCGCTTTCTT
TTAGCGAAAAAATGATCGCTTTAAAAAAGGTCCGCCAAGCCATCATTAAATTAAAACAAGGAGAATTAGTCGCCATATGA

Upstream 100 bases:

>100_bases
TAAAAAAGGGGCGTTATTGACAAACCTTTTTTAAGATTTTATTTATTGAGTATAATGCGTTGAAGCGAGTTAAATTATAA
GAAAAAGTGAAAGCGTTAAA

Downstream 100 bases:

>100_bases
AAAAGATTAAAGCTTTAGCCTTATTTAGTGGGGGGCTAGATAGTTTGTTATCCATGAAATTACTCATTGATCAAGGCATT
GAAGTAACCGCCTTACACTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MILKSSVDRLLQVADIVEVISSYVDLRKTGSNYMACCPFHDERSASFSVNQAKGFYHCFGCGASGDSIQFVMEFEKLSFV
EALEKLAHRFNVTLEYDKGVYYDYKEDYHLLEMVSSLYQEELFNAPFFLDYLQKRGLNIESIRAFKLGLCVNRIYHGIEN
KGLNKDKLIELGVLGKNDNNQKTYLRFLDRIMFPIYNPSAQVVGFGGRTLKEKGAKYINSPQSKLFDKSSLLYGYHLAKE
SIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEIFLSYDGDKAGRNAAYKASLMLAKEQRKGG
VILFENNLDPADMIANNQIETLKNWLSHPIAFIEFVLRHMASSYVLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAT
LLQVPSHVLGIKERSSFQPFYSKTEKSNYSQKFVHVSNTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSPLSFSEKMIALKKVRQAIIKLKQGELVAI

Sequences:

>Translated_559_residues
MILKSSVDRLLQVADIVEVISSYVDLRKTGSNYMACCPFHDERSASFSVNQAKGFYHCFGCGASGDSIQFVMEFEKLSFV
EALEKLAHRFNVTLEYDKGVYYDYKEDYHLLEMVSSLYQEELFNAPFFLDYLQKRGLNIESIRAFKLGLCVNRIYHGIEN
KGLNKDKLIELGVLGKNDNNQKTYLRFLDRIMFPIYNPSAQVVGFGGRTLKEKGAKYINSPQSKLFDKSSLLYGYHLAKE
SIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEIFLSYDGDKAGRNAAYKASLMLAKEQRKGG
VILFENNLDPADMIANNQIETLKNWLSHPIAFIEFVLRHMASSYVLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAT
LLQVPSHVLGIKERSSFQPFYSKTEKSNYSQKFVHVSNTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSPLSFSEKMIALKKVRQAIIKLKQGELVAI
>Mature_559_residues
MILKSSVDRLLQVADIVEVISSYVDLRKTGSNYMACCPFHDERSASFSVNQAKGFYHCFGCGASGDSIQFVMEFEKLSFV
EALEKLAHRFNVTLEYDKGVYYDYKEDYHLLEMVSSLYQEELFNAPFFLDYLQKRGLNIESIRAFKLGLCVNRIYHGIEN
KGLNKDKLIELGVLGKNDNNQKTYLRFLDRIMFPIYNPSAQVVGFGGRTLKEKGAKYINSPQSKLFDKSSLLYGYHLAKE
SIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEIFLSYDGDKAGRNAAYKASLMLAKEQRKGG
VILFENNLDPADMIANNQIETLKNWLSHPIAFIEFVLRHMASSYVLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAT
LLQVPSHVLGIKERSSFQPFYSKTEKSNYSQKFVHVSNTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSPLSFSEKMIALKKVRQAIIKLKQGELVAI

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=447, Percent_Identity=32.662192393736, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 63777; Mature: 63777

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSSVDRLLQVADIVEVISSYVDLRKTGSNYMACCPFHDERSASFSVNQAKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHCCCEEEEEE
CGASGDSIQFVMEFEKLSFVEALEKLAHRFNVTLEYDKGVYYDYKEDYHLLEMVSSLYQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHH
ELFNAPFFLDYLQKRGLNIESIRAFKLGLCVNRIYHGIENKGLNKDKLIELGVLGKNDNN
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCC
QKTYLRFLDRIMFPIYNPSAQVVGFGGRTLKEKGAKYINSPQSKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHH
SIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEIFLSYDGDKA
HHHHHHHEEEECCHHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRKGGVILFENNLDPADMIANNQIETLKNWLSHPIAFIEFVLRHM
CCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHH
ASSYVLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIATLLQVPSHVLGIKERSSFQPF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHH
YSKTEKSNYSQKFVHVSNTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
HHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSPLSFSEKMIA
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKKVRQAIIKLKQGELVAI
HHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MILKSSVDRLLQVADIVEVISSYVDLRKTGSNYMACCPFHDERSASFSVNQAKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHCCCEEEEEE
CGASGDSIQFVMEFEKLSFVEALEKLAHRFNVTLEYDKGVYYDYKEDYHLLEMVSSLYQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHH
ELFNAPFFLDYLQKRGLNIESIRAFKLGLCVNRIYHGIENKGLNKDKLIELGVLGKNDNN
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCC
QKTYLRFLDRIMFPIYNPSAQVVGFGGRTLKEKGAKYINSPQSKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHH
SIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEIFLSYDGDKA
HHHHHHHEEEECCHHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRKGGVILFENNLDPADMIANNQIETLKNWLSHPIAFIEFVLRHM
CCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHH
ASSYVLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIATLLQVPSHVLGIKERSSFQPF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHH
YSKTEKSNYSQKFVHVSNTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
HHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSPLSFSEKMIA
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKKVRQAIIKLKQGELVAI
HHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]