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The map label for this gene is dniR [H]

Identifier: 109948153

GI number: 109948153

Start: 1460826

End: 1461932

Strand: Reverse

Name: dniR [H]

Synonym: Hac_1679

Alternate gene names: 109948153

Gene position: 1461932-1460826 (Counterclockwise)

Preceding gene: 109948154

Following gene: 109948152

Centisome position: 94.08

GC content: 39.3

Gene sequence:

>1107_bases
ATGAAATATTTTAATACAAAATGGCTGTTTTTTTTAATGACTCATTGGCTTTTATTGGCTTCTTTGAGCCAAGCTAAGAT
CGCTTTTGAATCTAGTATTGACACCAAAGCGTTAGAAGCTTTTGGGGTTAATGCGAGCTTTTTATCCCAAATGCCAAGCG
TTTTAAAAAAAATGAATGAAGAAGAGGAATGGCGGAGGTTGGTCAAAAGGTTTGATGCGAATTACCAATTTATCCCCATC
ATAAAAAACATGTTAAGAGAAGCGAGCGTCCCACAGGAATTCTTGTTTTTAGCGATGGCAGAGTCTCACTTTTCAACAAG
GGCTTATAGCAGGAAAAGAGCAGTGGGGATTTGGCAGTTCATGCCAAGCACGGCGAAAGAATTGCACCTTAAAGTCAATC
ATTACATTGACGAAAGAAGAGATCCCATTAAAAGCACCAAAGCGGCTATCGTTTATTTAAAACAGTTATACAAGCAAACT
GGAGAGTGGTATTTGGTCGCTATGGCGTATAATTACGGCTTACGAAAGGTTCAAAACGCCATTAAAGCCGCCGGCACTTC
GGATATTAAGATCTTGCTTGATGAGGATAAAAAATACCTCCCCAAAGAAACGAGGGATTATATCCGCTCCATTTTAAGCC
TAGCGTTAAAATTCAACAACCTAGACAATCTCAAAAACAAAGAGTATTTGCTCAATCGTGGGGCGAGGGTGAGTTTGGTT
GGCGTTCCGTTTAAAAGGCATATCTCTTTAGTCCAAGTCGCCAAAAGTTTAAACTTGAGTTTAGAGACCCTAAAATCTTA
CAACCACCAATTCCGCTACAATGTTTTGCCCTCTAAAGATCCCACTTACACCATCTATATCCCTTATGAAAAACTCGCTC
TTTTCAAACAACACCAGTTTAAGCAGCATAAAAGTGCTCAAACCAAAAGCCCTTTTATCACCCATGTCGTCTTGCCTAAA
GAAACCCTATTTTCTATTGCTAAACGCTACCATGTCAGCATTTCAAGCATCCAATTAGCCAACAATCTTAAAGATTCCAA
TATCTTTACCCACCAACGCTTGCTCATCCCTGCCAATAAAAAACTCATCGCTATAAGGGAATTTTAA

Upstream 100 bases:

>100_bases
TTCCCTTAAAAGTAAAGTTGTATTTAAAAAAGTATTATTCAAGTTTGGTATAATTAGAGCCATTATTAAACTTATCAAAT
TATTAGGATTTTAAAAAAGA

Downstream 100 bases:

>100_bases
TGGGGTGGGCGTTGAAAAAAATTTGTTTTTTAGGGCTTGTCCTTTTAATTAGTGCTTGCACGACTAAAAACGATGGGGTC
AAAAATTTATCTTACAAGCA

Product: regulatory protein DniR

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKYFNTKWLFFLMTHWLLLASLSQAKIAFESSIDTKALEAFGVNASFLSQMPSVLKKMNEEEEWRRLVKRFDANYQFIPI
IKNMLREASVPQEFLFLAMAESHFSTRAYSRKRAVGIWQFMPSTAKELHLKVNHYIDERRDPIKSTKAAIVYLKQLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETRDYIRSILSLALKFNNLDNLKNKEYLLNRGARVSLV
GVPFKRHISLVQVAKSLNLSLETLKSYNHQFRYNVLPSKDPTYTIYIPYEKLALFKQHQFKQHKSAQTKSPFITHVVLPK
ETLFSIAKRYHVSISSIQLANNLKDSNIFTHQRLLIPANKKLIAIREF

Sequences:

>Translated_368_residues
MKYFNTKWLFFLMTHWLLLASLSQAKIAFESSIDTKALEAFGVNASFLSQMPSVLKKMNEEEEWRRLVKRFDANYQFIPI
IKNMLREASVPQEFLFLAMAESHFSTRAYSRKRAVGIWQFMPSTAKELHLKVNHYIDERRDPIKSTKAAIVYLKQLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETRDYIRSILSLALKFNNLDNLKNKEYLLNRGARVSLV
GVPFKRHISLVQVAKSLNLSLETLKSYNHQFRYNVLPSKDPTYTIYIPYEKLALFKQHQFKQHKSAQTKSPFITHVVLPK
ETLFSIAKRYHVSISSIQLANNLKDSNIFTHQRLLIPANKKLIAIREF
>Mature_368_residues
MKYFNTKWLFFLMTHWLLLASLSQAKIAFESSIDTKALEAFGVNASFLSQMPSVLKKMNEEEEWRRLVKRFDANYQFIPI
IKNMLREASVPQEFLFLAMAESHFSTRAYSRKRAVGIWQFMPSTAKELHLKVNHYIDERRDPIKSTKAAIVYLKQLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETRDYIRSILSLALKFNNLDNLKNKEYLLNRGARVSLV
GVPFKRHISLVQVAKSLNLSLETLKSYNHQFRYNVLPSKDPTYTIYIPYEKLALFKQHQFKQHKSAQTKSPFITHVVLPK
ETLFSIAKRYHVSISSIQLANNLKDSNIFTHQRLLIPANKKLIAIREF

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=300, Percent_Identity=30.6666666666667, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42997; Mature: 42997

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFNTKWLFFLMTHWLLLASLSQAKIAFESSIDTKALEAFGVNASFLSQMPSVLKKMNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
EEEWRRLVKRFDANYQFIPIIKNMLREASVPQEFLFLAMAESHFSTRAYSRKRAVGIWQF
HHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPSTAKELHLKVNHYIDERRDPIKSTKAAIVYLKQLYKQTGEWYLVAMAYNYGLRKVQNA
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
IKAAGTSDIKILLDEDKKYLPKETRDYIRSILSLALKFNNLDNLKNKEYLLNRGARVSLV
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEE
GVPFKRHISLVQVAKSLNLSLETLKSYNHQFRYNVLPSKDPTYTIYIPYEKLALFKQHQF
ECCHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHH
KQHKSAQTKSPFITHVVLPKETLFSIAKRYHVSISSIQLANNLKDSNIFTHQRLLIPANK
HHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
KLIAIREF
CEEEEECC
>Mature Secondary Structure
MKYFNTKWLFFLMTHWLLLASLSQAKIAFESSIDTKALEAFGVNASFLSQMPSVLKKMNE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
EEEWRRLVKRFDANYQFIPIIKNMLREASVPQEFLFLAMAESHFSTRAYSRKRAVGIWQF
HHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPSTAKELHLKVNHYIDERRDPIKSTKAAIVYLKQLYKQTGEWYLVAMAYNYGLRKVQNA
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
IKAAGTSDIKILLDEDKKYLPKETRDYIRSILSLALKFNNLDNLKNKEYLLNRGARVSLV
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEE
GVPFKRHISLVQVAKSLNLSLETLKSYNHQFRYNVLPSKDPTYTIYIPYEKLALFKQHQF
ECCHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHH
KQHKSAQTKSPFITHVVLPKETLFSIAKRYHVSISSIQLANNLKDSNIFTHQRLLIPANK
HHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
KLIAIREF
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]