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The map label for this gene is metB [H]

Identifier: 109947959

GI number: 109947959

Start: 1270532

End: 1271674

Strand: Reverse

Name: metB [H]

Synonym: Hac_1464

Alternate gene names: 109947959

Gene position: 1271674-1270532 (Counterclockwise)

Preceding gene: 109947960

Following gene: 109947958

Centisome position: 81.84

GC content: 45.76

Gene sequence:

>1143_bases
ATGCACATGCAAACCAAATTGATCCATGGGGGCATTAGTGAGGACGCAACAACGGGGGCGGTAAGCGTGCCTATTTATCA
AACTTCCACCTACCGCCAAGACGCCATAGGCCGTCCAAAGGGCTATGAATACTCTCGCTCAGGCAACCCTACGCGCTTTG
CATTAGAAGAACTTATCGCCGATTTAGAAGGGGGGGTTAAGGGGTTTGCTTTTGCCTCTGGTTTAGCCGGAATCCACGCT
GTCTTTTCTCTCTTTCAATCAGGCGATCATGTGTTATTGGGCGATGATGTCTATGGGGGGACTTTTCGCTTATTTGACAA
GGTGCTTTCTAAAAATGGGCTTTCTTGCACCATTATAGAGACTAGCGATATACCTCAAATAAAAAAGGCTATTAAACCCA
ACACCAAAGCCCTCTATTTAGAATCCCCTAGTAACCCCTTGCTTAAAATCACGGATTTGAAGCAATGTGCAAGTCTTGCT
AAAGCTCATGGTCTGCTCACTATCGTGGATAACACCTTTGCCACGCCTTATTACCAAAACCCTCTTCTTTTGGGAGCGGA
CATTGTAGTGCATAGCGGCACAAAATATTTAGGCGGGCATAGCGATGTAGTCGCTGGGCTTGTAACCACTAATAATGAAG
CACTAGCCCAAGAGATCGCCTTTTTCCAAAACGCTATTGGGGGGGTGCTAGGTCCTCAAGATAGTTGGCTGTTGCAAAGA
GGGATTAAAACGCTAGGATTGCGCATGGAAGCCCATCAAAAAAACGCCCTTTGTGTGGCTGAGTTTTTAGAAAAACACCC
TAAAGTAGAGAGGGTTTATTATCCAGGCCTGCCCACTCACCTTAATCACGATCTAGCCAAAGCTCAGATGCGTGGTTTTA
GCGGAATGCTTTCTTTCACCCTCAAAAACGACAACGAAGCAACTCCTTTTGTAGAGAATCTTAAATTATTTATTTTAGGC
GAGAGTTTGGGTGGGGTGGAAAGTTTGGTAGGCATTCCAGTGCTCATGACCCATGCGTGTATCCCTAAAGTGCAACGAGA
AGCTGCAGGGATTAGAGATGGTTTGGTGCGCTTGTCTGTGGGGATTGAGCATGAAAAGGATTTGTTAGAAGATTTAGAGC
AAGCGTTCGCTAAACTAGGCTAA

Upstream 100 bases:

>100_bases
CCCAATGGTAGCCAAGTTTTAACCATTTTTCCGGATGTTGCCGATCGTTATCTCTCAAAAGGTATTTATTCATAAAAAAT
TCAATAAAAAAGGAAAAATC

Downstream 100 bases:

>100_bases
AGTTTCATCACAATTTATGGGTAAAGGAATCAAAAATGAAAACGCCAAACACAAAAATGAATGTAGAAAGTTTTAATTTG
GATCACACCAAAGTCAAAGC

Product: cystathionine gamma-synthase/cystathionine beta-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGRPKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLFQSGDHVLLGDDVYGGTFRLFDKVLSKNGLSCTIIETSDIPQIKKAIKPNTKALYLESPSNPLLKITDLKQCASLA
KAHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVERVYYPGLPTHLNHDLAKAQMRGFSGMLSFTLKNDNEATPFVENLKLFILG
ESLGGVESLVGIPVLMTHACIPKVQREAAGIRDGLVRLSVGIEHEKDLLEDLEQAFAKLG

Sequences:

>Translated_380_residues
MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGRPKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLFQSGDHVLLGDDVYGGTFRLFDKVLSKNGLSCTIIETSDIPQIKKAIKPNTKALYLESPSNPLLKITDLKQCASLA
KAHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVERVYYPGLPTHLNHDLAKAQMRGFSGMLSFTLKNDNEATPFVENLKLFILG
ESLGGVESLVGIPVLMTHACIPKVQREAAGIRDGLVRLSVGIEHEKDLLEDLEQAFAKLG
>Mature_380_residues
MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGRPKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLFQSGDHVLLGDDVYGGTFRLFDKVLSKNGLSCTIIETSDIPQIKKAIKPNTKALYLESPSNPLLKITDLKQCASLA
KAHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVERVYYPGLPTHLNHDLAKAQMRGFSGMLSFTLKNDNEATPFVENLKLFILG
ESLGGVESLVGIPVLMTHACIPKVQREAAGIRDGLVRLSVGIEHEKDLLEDLEQAFAKLG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=363, Percent_Identity=48.7603305785124, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=44.3526170798898, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI25453487, Length=362, Percent_Identity=43.9226519337017, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=41.9786096256684, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=31.3924050632911, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=367, Percent_Identity=47.9564032697548, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17538055, Length=367, Percent_Identity=47.9564032697548, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI71980708, Length=390, Percent_Identity=39.7435897435897, Blast_Score=281, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=46.4935064935065, Blast_Score=322, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=32.3383084577114, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=33.4872979214781, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=35.7575757575758, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.3670886075949, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323557, Length=255, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322970, Length=302, Percent_Identity=27.4834437086093, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322590, Length=257, Percent_Identity=30.3501945525292, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=49.4708994708995, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41141; Mature: 41141

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGRPKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLFQSGDHVLLGDDVYGGTFRLFDKVLSKNGLSCTIIE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEE
TSDIPQIKKAIKPNTKALYLESPSNPLLKITDLKQCASLAKAHGLLTIVDNTFATPYYQN
CCCCHHHHHHHCCCCCEEEEECCCCCCEEECCHHHHHHHHHHCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLGLRMEAHQKNALCVAEFLEKHPKVERVYYPGLPTHLNHDLAKAQMRGFSGMLSFT
HHHHHCCEEEHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
LKNDNEATPFVENLKLFILGESLGGVESLVGIPVLMTHACIPKVQREAAGIRDGLVRLSV
ECCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEE
GIEHEKDLLEDLEQAFAKLG
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGRPKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLFQSGDHVLLGDDVYGGTFRLFDKVLSKNGLSCTIIE
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEE
TSDIPQIKKAIKPNTKALYLESPSNPLLKITDLKQCASLAKAHGLLTIVDNTFATPYYQN
CCCCHHHHHHHCCCCCEEEEECCCCCCEEECCHHHHHHHHHHCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLGLRMEAHQKNALCVAEFLEKHPKVERVYYPGLPTHLNHDLAKAQMRGFSGMLSFT
HHHHHCCEEEHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
LKNDNEATPFVENLKLFILGESLGGVESLVGIPVLMTHACIPKVQREAAGIRDGLVRLSV
ECCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEE
GIEHEKDLLEDLEQAFAKLG
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]