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The map label for this gene is greA

Identifier: 109947754

GI number: 109947754

Start: 1063949

End: 1064443

Strand: Reverse

Name: greA

Synonym: Hac_1229

Alternate gene names: 109947754

Gene position: 1064443-1063949 (Counterclockwise)

Preceding gene: 109947755

Following gene: 109947753

Centisome position: 68.5

GC content: 37.58

Gene sequence:

>495_bases
ATGAATAGAGAACCTATGAGTATGCATGGATACAATAAAATCTGTGCGGAATTGAAGCAATTAAAAGAGGTGGAACGGCC
TAATATTGTGAAAGAAATTGATATTGCTAGAGGGCATGGGGATTTGAAAGAAAACGCTGAATACCATGCCGCTAAAGAAA
AACAACGCTTCATTGAGGCAAGAATCGTGGATTTGAGCGAGATCATTTCTAACGCTCAAGTGATTGACCCAGGTGCTTTG
ATGCATAATAAAGTGAGTTTTGGAAGCACGATTAAAATCCTTAATTTAGATAACGATAAAGAGTTTTCTTATACGATAGT
AGGGAGCGTAGAGAGTGATCCGGCTAAAGGGTTGATTTCTTTTGGTTCGCCGATCGCTAAGAGTTTGATCGGTAAGAGCA
AGGGTGATGTGGCAAGCATTCAATTACCTAATGGGGAGAGCGATTTTGAAATTTTAGACATTTATTATAAAGAGATTTGT
TTTGATGAAAATTAA

Upstream 100 bases:

>100_bases
ATGCATGGGAGCGCGAGAAAAATCGCTAGTGAAATAGCTTTTTTGCTGAATTTAACTTAATTTTTACTATAATCTCTACT
GAGTAGGATTAGGAGATTGA

Downstream 100 bases:

>100_bases
AATCCAAAAAATCCACCCAAACGCCCTTATCCCTAAATACCAAACTGAAGGTTCTTCAGGCTTTGATTTGCATGCGGTAG
AAAATGCAGTGATCAAACCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNREPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIISNAQVIDPGAL
MHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDVASIQLPNGESDFEILDIYYKEIC
FDEN

Sequences:

>Translated_164_residues
MNREPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIISNAQVIDPGAL
MHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDVASIQLPNGESDFEILDIYYKEIC
FDEN
>Mature_164_residues
MNREPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIISNAQVIDPGAL
MHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDVASIQLPNGESDFEILDIYYKEIC
FDEN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=50.9554140127389, Blast_Score=153, Evalue=6e-39,
Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=33.7748344370861, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_HELAH (Q17WJ3)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664982.1
- ProteinModelPortal:   Q17WJ3
- SMR:   Q17WJ3
- STRING:   Q17WJ3
- GeneID:   4177271
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1229
- NMPDR:   fig|382638.8.peg.1194
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q17WJ3
- ProtClustDB:   PRK00226
- BioCyc:   HACI382638:HAC_1229-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18287; Mature: 18287

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHH
RIVDLSEIISNAQVIDPGALMHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLIS
HHHHHHHHHCCCEEECCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
FGSPIAKSLIGKSKGDVASIQLPNGESDFEILDIYYKEICFDEN
CCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNREPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHH
RIVDLSEIISNAQVIDPGALMHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLIS
HHHHHHHHHCCCEEECCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
FGSPIAKSLIGKSKGDVASIQLPNGESDFEILDIYYKEICFDEN
CCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA