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The map label for this gene is rlmB [H]

Identifier: 109947425

GI number: 109947425

Start: 763358

End: 764041

Strand: Reverse

Name: rlmB [H]

Synonym: Hac_0872

Alternate gene names: 109947425

Gene position: 764041-763358 (Counterclockwise)

Preceding gene: 109947426

Following gene: 109947424

Centisome position: 49.17

GC content: 38.45

Gene sequence:

>684_bases
ATGCAAGCAGTGATTTATGGCAAACAGGTGGTTATGCATATTCTAAACTCTCATCAAGAAAAGTTGCAAGAAATCTATCT
TTCTAAAGAAATAGACAAGAAACTTTTTTTCGCACTCAAAAAAGCATGCCCAAACATCATTAAAGTGGATAATAAAAAAG
CGCAAAGCTTGGCTAAGGGGGGGAATCATCAAGGGATTTTAGCTAGAGTGGAATTGCCCATAGCCATTTCTTTAAAAGAG
ATTAAAAAAGCTCAAAAACTTTTAGTGCTTTGCAGTATTACGGATGTGGGGAATATTGGGGGTATTTTTAGGAGCGCGTA
TTGCTTGGGTATGGATGGCGTTATTTTAGATTTTGCTAAAGAATTGGCTTATGAGGGGATTGTGCGATCGAGCTTGGGGC
TTATGTATGATTTGCCTTTTAGCGTCGTGCCTAACACGCTGGATTTAATCAATGAGTTAAAAACGAGTGGGTTTTTGTGT
TTAGGTGCGAACATGCAAGGCTCTAGCCCAAAAGAATATTTAGCCTTAAAAAAATGCGCTCTTTTTTTAGGGAGCGAGCA
TGAGGGGTTGTCTAAAAAAATTCTTGCTAAAATGGATACGATATTAAGTATAAAAATGCGGCGCGATTTTGACTCGCTCA
ATGTGAGTGTGGCAGCAGGGATCTTAATGGATAAAATCAACTAG

Upstream 100 bases:

>100_bases
AATTAAGACTAAAATTGAAGCCACTATGACACAACAAAACGCTAAAGAGCTTTATAGCCAGCGTTTGTTAGAAGAAAAAA
ATCAATAAAAGGGGTTTAGT

Downstream 100 bases:

>100_bases
GTGGTCAATGAATGGAACAGAATAAAAAAAGTTTAGAAAATTTAGATCTTTCTGATGTTCAAAAAAACTCTAAAGACATT
TCTGGTGTGGCTTTAGAAGA

Product: rRNA methylase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MQAVIYGKQVVMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGILARVELPIAISLKE
IKKAQKLLVLCSITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVVPNTLDLINELKTSGFLC
LGANMQGSSPKEYLALKKCALFLGSEHEGLSKKILAKMDTILSIKMRRDFDSLNVSVAAGILMDKIN

Sequences:

>Translated_227_residues
MQAVIYGKQVVMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGILARVELPIAISLKE
IKKAQKLLVLCSITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVVPNTLDLINELKTSGFLC
LGANMQGSSPKEYLALKKCALFLGSEHEGLSKKILAKMDTILSIKMRRDFDSLNVSVAAGILMDKIN
>Mature_227_residues
MQAVIYGKQVVMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKGGNHQGILARVELPIAISLKE
IKKAQKLLVLCSITDVGNIGGIFRSAYCLGMDGVILDFAKELAYEGIVRSSLGLMYDLPFSVVPNTLDLINELKTSGFLC
LGANMQGSSPKEYLALKKCALFLGSEHEGLSKKILAKMDTILSIKMRRDFDSLNVSVAAGILMDKIN

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24961; Mature: 24961

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVIYGKQVVMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCC
GNHQGILARVELPIAISLKEIKKAQKLLVLCSITDVGNIGGIFRSAYCLGMDGVILDFAK
CCCCCEEEEEECCEEEEHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHH
ELAYEGIVRSSLGLMYDLPFSVVPNTLDLINELKTSGFLCLGANMQGSSPKEYLALKKCA
HHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHH
LFLGSEHEGLSKKILAKMDTILSIKMRRDFDSLNVSVAAGILMDKIN
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQAVIYGKQVVMHILNSHQEKLQEIYLSKEIDKKLFFALKKACPNIIKVDNKKAQSLAKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCC
GNHQGILARVELPIAISLKEIKKAQKLLVLCSITDVGNIGGIFRSAYCLGMDGVILDFAK
CCCCCEEEEEECCEEEEHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHH
ELAYEGIVRSSLGLMYDLPFSVVPNTLDLINELKTSGFLCLGANMQGSSPKEYLALKKCA
HHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHH
LFLGSEHEGLSKKILAKMDTILSIKMRRDFDSLNVSVAAGILMDKIN
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]