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The map label for this gene is dat1 [H]

Identifier: 109947412

GI number: 109947412

Start: 748687

End: 749202

Strand: Reverse

Name: dat1 [H]

Synonym: Hac_0859

Alternate gene names: 109947412

Gene position: 749202-748687 (Counterclockwise)

Preceding gene: 109947413

Following gene: 109947386

Centisome position: 48.21

GC content: 33.33

Gene sequence:

>516_bases
ATGAGTAAGTCATTGATGGATTTATATCACTACTATTTTAAAACCCCTAAGAGTTTCCCCTTAGAGTATTTGCATTTGTG
TGCTAATGAGAGCCATTTATTAAGATTGGATTTTGATACGACAAATTTTTCTATCTCTATGAATACCCCATTAAAACTCA
GCGTTCAAGCCTTGGAGCGTTATTTCTTAGGACAACTTTTTGAATTTGATGTACCTTTAGATCTGATAGGGACTTTTTTT
CAAAAACAAGTTTGGTCTGCGTTATTAACTATCCCTTATGGTAAAATAAAAAGTTACGATGAAATCGCAAAGCTCATTAA
TAACCCTAAATCTTGCAGAGCTATTGGCAACGCTAATCACAATAACCCTATTTCTTTGATTGTGCCTTGTCATAGAGTGG
TGCGTAAAAATGGCACTTTAGGTGGGTATAATGGGGGCATAGAAGTCAAAAAGTGGCTGTTAGAATTTGAAAGCAAAATT
TTAAACCAACAAGCTAAAAACTTTTTAATTTTTTAA

Upstream 100 bases:

>100_bases
ATGAAAAAATGCATAAAGTTTTGATTTTAGGGGTGTATGGTTTGTCGCTATTGATTGTTTTATACAAACTCTTTGGCTAG
AATGTCGTTTTTTAGAAGAA

Downstream 100 bases:

>100_bases
AATGGGGTTTTAGTTGCTTTGTTTATAGATAGGGGTGGTATTTTTCAACTTTCTTCCCAAATACTCGTCGCTTCTTCTAG
ACGCTGTTTATCAAAATGCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSKSLMDLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDTTNFSISMNTPLKLSVQALERYFLGQLFEFDVPLDLIGTFF
QKQVWSALLTIPYGKIKSYDEIAKLINNPKSCRAIGNANHNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKI
LNQQAKNFLIF

Sequences:

>Translated_171_residues
MSKSLMDLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDTTNFSISMNTPLKLSVQALERYFLGQLFEFDVPLDLIGTFF
QKQVWSALLTIPYGKIKSYDEIAKLINNPKSCRAIGNANHNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKI
LNQQAKNFLIF
>Mature_170_residues
SKSLMDLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDTTNFSISMNTPLKLSVQALERYFLGQLFEFDVPLDLIGTFFQ
KQVWSALLTIPYGKIKSYDEIAKLINNPKSCRAIGNANHNNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKIL
NQQAKNFLIF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=86, Percent_Identity=40.6976744186046, Blast_Score=88, Evalue=4e-18,
Organism=Escherichia coli, GI1787596, Length=118, Percent_Identity=45.7627118644068, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1788542, Length=121, Percent_Identity=42.9752066115703, Blast_Score=105, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=45.2380952380952, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=45.2380952380952, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=128, Percent_Identity=34.375, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19710; Mature: 19579

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLMDLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDTTNFSISMNTPLKLSVQALER
CCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHH
YFLGQLFEFDVPLDLIGTFFQKQVWSALLTIPYGKIKSYDEIAKLINNPKSCRAIGNANH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCCHHHHCCCCCCC
NNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQQAKNFLIF
CCCEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKSLMDLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDTTNFSISMNTPLKLSVQALER
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHH
YFLGQLFEFDVPLDLIGTFFQKQVWSALLTIPYGKIKSYDEIAKLINNPKSCRAIGNANH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCCHHHHCCCCCCC
NNPISLIVPCHRVVRKNGTLGGYNGGIEVKKWLLEFESKILNQQAKNFLIF
CCCEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]