Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 109947386

GI number: 109947386

Start: 719342

End: 721306

Strand: Reverse

Name: ligA [H]

Synonym: Hac_0831

Alternate gene names: 109947386

Gene position: 721306-719342 (Counterclockwise)

Preceding gene: 109947412

Following gene: 109947381

Centisome position: 46.42

GC content: 37.81

Gene sequence:

>1965_bases
ATGATAAAAAGCCAAAAAGAATATTTAGAAAGAATTGAATATTTAAACACCCTATCGCACCACTATTACAACCTTGATGA
GCCGATCGTAAGCGATGCGGTCTATGATGAACTCTATCAAGAATTAAAAGCTTATGAAGAAAAAAACCCTAGCCAAATTC
AAGCCAACTCCCCCACCCAAAAAGTGGGGGCTACTGCACTCAACCAATTCAATAAAAACCCCCATTTAACGAGAATGTGG
AGTTTAGATGATGTATTCAATCATAACGAATTGCAAGCGTGGTTGCAACGCATTTTAAAAGCTTACCCTAGTGCTTCGTT
TGTGTGTTCGCCTAAACTTGATGGGGTTTCGCTCAATCTTTTGTATCAAAAAGGCAAGTTAATGAAGGCGACCACTAGGG
GCAATGGATTAGAAGGAGAATTAGTTACTACAAATGCCAAACATGTCGCTAATATCTCTCACACTATCCCTTATAATGAA
GAAATAGAAATCAGGGGCGAAGTGATCATTTCTAAAGAGGATTTTAACGCTTTGAATGAAGAACGCCTAAACGCTAACGA
ACCCCTATTCGCTAACCCTAGAAATGCCGCATCAGGGAGTTTGAGACAGCTTGATAGCAAAATCACTAAAAAGCGTAAAT
TGCAATTCATTCCTTGGGGCGTGGGCAAGCACTCTTTAAATTTCTTAAGCTTTAAGGAATGTTTGGATTTTATTGTCTCG
TTAGGTTTTAGCGCTATTAAACATTTAAGCCTAAACAAAAACCACCAAGAAATAGAAGCGAATTATCACACTCTCATTCA
AGAAAGAGAGAGATTTTTTGCCCTTTTAGACGGCATGGTGATCGTTGTGAATGAATTGAATATCCAAAAGGAGTTAGGTT
ATACGCAAAAATCCCCCAAATTCGCTTGCGCTTATAAATTCCCCGCTCTAGAAAAACACACCAAAATTGTAGGAGTTATT
AACCAAGTGGGGCGCAGTGGGGCGATCACCCCAGTCGCTCTTTTAGAGCCGGTAGAAATCGCTGGGGCTATCATCACTAA
AGCGACATTACACAATTATTCTGAAATTGAAAAAAAGAATATCATGCTCAATGATAGGGTCGTAGTCATTAGAAGCGGCG
ATGTGATCCCTAAAATCATCAAGCCTTTAGAGTCTTATAGGGACGGTTCGCAATGCAAAATTATACGCCCCAAAGTTTGC
CCCATATGCTCGCATGAACTCTTATGCGAAGAGATTTTTACTTATTGCCAAAACCTTAATTGCTCCGCAAGATTGAAAGA
AAGCTTGATTCATTTTGCTTCTAAAGACGCTTTAAACATTCAAGGCTTAGGCGATAAAGTCATAGAGCAGCTTTTTGAAG
AAAAACTCATTTTTAACGCCCTAGATTTGTATGCTTTAAAATTAGAAGATTTAATGCGGCTAGACAAATTCAAAATTAAA
AAAGCTCAAAATTTATTAGACGCTATTCAAAAGAGCAAAAACCCCCCTTTATGGCGTTTGATCAATGCTTTAGGGATTGA
ACATATCGGTAAGGGAGCGAGTAAAACGTTGGCCAAATACGGCTTATATGTGTTAGAAAAAAGCGAAGATGAGTTTTTAG
AAATGGAGGGCTTTGGGGTGGAAATGGCGCGTTCTTTAGTCAATTTTTATGCGAGCAATCAAGAGTTTATCCAATCATTA
TTTGATTTATTAAACCCTAAAAATAGCGATATAATTGAAGAAAAGCAAGAAAGCTCTTCAATTTTTAGCCATAAAACGAT
TGTTTTAACCGGCACGCTCTCTAAACCACGGCAAGAATACGCTCAAATCTTAGAAAATTTAGGGGCAAAAATTTCTTCAA
GCGTGAGCGCTAAAACCGATTTTTTGATCGTTGGAGAAAATGCCGGCTCAAAACTTTCTCTAGCACAAAAACATGGCGTT
AACATTTTGAATGAAGAAGAATTATTAAAATATCTTAAAGAATGA

Upstream 100 bases:

>100_bases
GTCGTTTTGTCAATGTCTTTCACCACCTATTTCCCCTAAACCCTATCAAAGATTAAATGTTATAATACTACCCTAATTTA
GTTTAAAAGGTTTTTAGAAT

Downstream 100 bases:

>100_bases
GAATAAAACCACTAAAAAAGCTGTTTTTAAGAAACAACCACAACCGCTATCGCAAAACCAGCGTCATGGCTGATACTTGC
ACTCAAACTTTGGATGTTAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MIKSQKEYLERIEYLNTLSHHYYNLDEPIVSDAVYDELYQELKAYEEKNPSQIQANSPTQKVGATALNQFNKNPHLTRMW
SLDDVFNHNELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQKGKLMKATTRGNGLEGELVTTNAKHVANISHTIPYNE
EIEIRGEVIISKEDFNALNEERLNANEPLFANPRNAASGSLRQLDSKITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIKHLSLNKNHQEIEANYHTLIQERERFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQCKIIRPKVC
PICSHELLCEEIFTYCQNLNCSARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLAKYGLYVLEKSEDEFLEMEGFGVEMARSLVNFYASNQEFIQSL
FDLLNPKNSDIIEEKQESSSIFSHKTIVLTGTLSKPRQEYAQILENLGAKISSSVSAKTDFLIVGENAGSKLSLAQKHGV
NILNEEELLKYLKE

Sequences:

>Translated_654_residues
MIKSQKEYLERIEYLNTLSHHYYNLDEPIVSDAVYDELYQELKAYEEKNPSQIQANSPTQKVGATALNQFNKNPHLTRMW
SLDDVFNHNELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQKGKLMKATTRGNGLEGELVTTNAKHVANISHTIPYNE
EIEIRGEVIISKEDFNALNEERLNANEPLFANPRNAASGSLRQLDSKITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIKHLSLNKNHQEIEANYHTLIQERERFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQCKIIRPKVC
PICSHELLCEEIFTYCQNLNCSARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLAKYGLYVLEKSEDEFLEMEGFGVEMARSLVNFYASNQEFIQSL
FDLLNPKNSDIIEEKQESSSIFSHKTIVLTGTLSKPRQEYAQILENLGAKISSSVSAKTDFLIVGENAGSKLSLAQKHGV
NILNEEELLKYLKE
>Mature_654_residues
MIKSQKEYLERIEYLNTLSHHYYNLDEPIVSDAVYDELYQELKAYEEKNPSQIQANSPTQKVGATALNQFNKNPHLTRMW
SLDDVFNHNELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQKGKLMKATTRGNGLEGELVTTNAKHVANISHTIPYNE
EIEIRGEVIISKEDFNALNEERLNANEPLFANPRNAASGSLRQLDSKITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
LGFSAIKHLSLNKNHQEIEANYHTLIQERERFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGVI
NQVGRSGAITPVALLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQCKIIRPKVC
PICSHELLCEEIFTYCQNLNCSARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLAKYGLYVLEKSEDEFLEMEGFGVEMARSLVNFYASNQEFIQSL
FDLLNPKNSDIIEEKQESSSIFSHKTIVLTGTLSKPRQEYAQILENLGAKISSSVSAKTDFLIVGENAGSKLSLAQKHGV
NILNEEELLKYLKE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=38.4155455904335, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI87082305, Length=559, Percent_Identity=20.2146690518784, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74019; Mature: 74019

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSQKEYLERIEYLNTLSHHYYNLDEPIVSDAVYDELYQELKAYEEKNPSQIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
KVGATALNQFNKNPHLTRMWSLDDVFNHNELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HHHHHHHHHCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQKGKLMKATTRGNGLEGELVTTNAKHVANISHTIPYNEEIEIRGEVIISKEDFNALNE
EEECCCEEEEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCEEEEEEEEEECHHCCHHHH
ERLNANEPLFANPRNAASGSLRQLDSKITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHH
LGFSAIKHLSLNKNHQEIEANYHTLIQERERFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGVINQVGRSGAITPVALLEPVEIAGAIITKATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
IMLNDRVVVIRSGDVIPKIIKPLESYRDGSQCKIIRPKVCPICSHELLCEEIFTYCQNLN
EEEECEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCC
CSARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLAKYGLYVLEKSEDEFLEMEGFGV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCCHHEECCCCCH
EMARSLVNFYASNQEFIQSLFDLLNPKNSDIIEEKQESSSIFSHKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQILENLGAKISSSVSAKTDFLIVGENAGSKLSLAQKHGVNILNEEELLKYLKE
HHHHHHHCCHHHCCCCCCCCEEEEECCCCCCEEHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIKSQKEYLERIEYLNTLSHHYYNLDEPIVSDAVYDELYQELKAYEEKNPSQIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
KVGATALNQFNKNPHLTRMWSLDDVFNHNELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HHHHHHHHHCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQKGKLMKATTRGNGLEGELVTTNAKHVANISHTIPYNEEIEIRGEVIISKEDFNALNE
EEECCCEEEEECCCCCCCCCEEECCCHHHHCCCCCCCCCCCEEEEEEEEEECHHCCHHHH
ERLNANEPLFANPRNAASGSLRQLDSKITKKRKLQFIPWGVGKHSLNFLSFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHH
LGFSAIKHLSLNKNHQEIEANYHTLIQERERFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGVINQVGRSGAITPVALLEPVEIAGAIITKATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
IMLNDRVVVIRSGDVIPKIIKPLESYRDGSQCKIIRPKVCPICSHELLCEEIFTYCQNLN
EEEECEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCC
CSARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLAKYGLYVLEKSEDEFLEMEGFGV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCCCHHEECCCCCH
EMARSLVNFYASNQEFIQSLFDLLNPKNSDIIEEKQESSSIFSHKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQILENLGAKISSSVSAKTDFLIVGENAGSKLSLAQKHGVNILNEEELLKYLKE
HHHHHHHCCHHHCCCCCCCCEEEEECCCCCCEEHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA