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The map label for this gene is tgt

Identifier: 109947132

GI number: 109947132

Start: 463980

End: 465095

Strand: Reverse

Name: tgt

Synonym: Hac_0541

Alternate gene names: 109947132

Gene position: 465095-463980 (Counterclockwise)

Preceding gene: 109947146

Following gene: 109947110

Centisome position: 29.93

GC content: 41.22

Gene sequence:

>1116_bases
ATGGATTTTCAACTCCAGGCTGTCGACAAACATGCACGAGCTGGTATTTTAAATTTAGTGCATTCTCAAGTAGAGACACC
TATTTTTATGCCTGTAGGCACACAAGGCTGTATCAAATCTTTAGACGCTATTGACATGCAAGAACGCTTGAGCGCTAAAC
TCATTTTAGCCAACACTTACCACATGTATTTAAGACCGGGTGAGAAAGTTATCGAGCAATTAGGAGGCTTGCATCATTTC
GCTCAATTCCATGGGAGTTTTCTAACGGATAGTGGGGGGTTTCAAGCCTTTAGTTTGAGCGATAATGTTAAATTGCAAGA
AGATGGGATTGTTTTTAAATCCCATCTTGATGGGAGCAAGCATTTCTTCACGCCCACTAAAGTTTTGGATATTCAATATT
CTTTAAATAGCGATATTATGATGGTTTTAGACGATCTAGTGGGCTTACCTGCTCCACTAAAACGCCTTGAAGAATCCATC
AAACGAAGCGCTAAATGGGCAAATATTAGCCTAGAATACCACAAAGAAAACAACCGCCCTAACAACAACCTTTTTGCCAT
TATCCAAGGCGGCACCCATTTGAAAATGCGTAGTCTTAGCGTGGGATTAACGCATGGTGGTTTTGATGGCTATGCGATAG
GCGGTTTAGCGGTGGGCGAAAGCACAAATGAAATGCTAGAAACTATCGCTCACACCGCCCCCTTACTCCCTAAAGACAAA
CCTCGCTACTTAATGGGCGTAGGCACGCCTGAAAACATTTTAGACGCTATCAGTTTAGGGGTGGATATGTTTGATTGCGT
GATGCCCACAAGAAACGCCAGAAACGCCACCCTTTTCACGCATTCTGGCAAAATCTCTATCAAAAACGCGCCCTATAAAT
TGGACAACACCCCCATTGAAGAAAATTGCACTTGTTACACTTGCAAACGCTATTCTAAAGCTTATTTGCACCATCTCTTT
AGGGCTAAAGAACTCACTTACGCTCGTTTAGCGAGCTTGCACAATCTGCATTTTTATTTAGAGCTGGTTAAAAACGCTAG
AAACGCCATTTTAGAAAAGCGGTTTTTAAGTTTTAAAAAAGAGTTTTTAGAAAGATATACAATGGATCGTCAATAA

Upstream 100 bases:

>100_bases
TGCTACAATGCCATCTAAAATAAGGGCGATTTTTTTCAATTCCAATCCAAACTTCCTAAAAAATGATTACGCCATTATAT
CCAAGATTAAGGCTTAAACA

Downstream 100 bases:

>100_bases
AAAATATCAAAGAGTTTGACGCTAGTCAGGTGATAACTTTTTAAAAAATGGGATTTTTAAACCTTATCTTTATGGTAAAA
AACTCATTTTCTTAAGGGAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDFQLQAVDKHARAGILNLVHSQVETPIFMPVGTQGCIKSLDAIDMQERLSAKLILANTYHMYLRPGEKVIEQLGGLHHF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHLDGSKHFFTPTKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANISLEYHKENNRPNNNLFAIIQGGTHLKMRSLSVGLTHGGFDGYAIGGLAVGESTNEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCTCYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLERYTMDRQ

Sequences:

>Translated_371_residues
MDFQLQAVDKHARAGILNLVHSQVETPIFMPVGTQGCIKSLDAIDMQERLSAKLILANTYHMYLRPGEKVIEQLGGLHHF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHLDGSKHFFTPTKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANISLEYHKENNRPNNNLFAIIQGGTHLKMRSLSVGLTHGGFDGYAIGGLAVGESTNEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCTCYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLERYTMDRQ
>Mature_371_residues
MDFQLQAVDKHARAGILNLVHSQVETPIFMPVGTQGCIKSLDAIDMQERLSAKLILANTYHMYLRPGEKVIEQLGGLHHF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHLDGSKHFFTPTKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANISLEYHKENNRPNNNLFAIIQGGTHLKMRSLSVGLTHGGFDGYAIGGLAVGESTNEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCTCYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLERYTMDRQ

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=40, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=46.866485013624, Blast_Score=350, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=37.7717391304348, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=213, Percent_Identity=25.8215962441315, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=41.0112359550562, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI21355311, Length=385, Percent_Identity=25.974025974026, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_HELAH (Q17YB5)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664360.1
- ProteinModelPortal:   Q17YB5
- SMR:   Q17YB5
- STRING:   Q17YB5
- GeneID:   4177473
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0541
- NMPDR:   fig|382638.8.peg.525
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q17YB5
- ProtClustDB:   PRK00112
- BioCyc:   HACI382638:HAC_0541-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41819; Mature: 41819

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: ACT_SITE 90-90 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLQAVDKHARAGILNLVHSQVETPIFMPVGTQGCIKSLDAIDMQERLSAKLILANTY
CCCEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHCEEEEEEEEE
HMYLRPGEKVIEQLGGLHHFAQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHLDGSK
EEEECCHHHHHHHHCCHHHHHHHCCCEEECCCCEEEEECCCCCEEECCCEEEEECCCCCC
HFFTPTKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANISLEYHKENNRP
EEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
NNNLFAIIQGGTHLKMRSLSVGLTHGGFDGYAIGGLAVGESTNEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEEEECCCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCTCYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLERYTMDRQ
HHHHHHCCCCC
>Mature Secondary Structure
MDFQLQAVDKHARAGILNLVHSQVETPIFMPVGTQGCIKSLDAIDMQERLSAKLILANTY
CCCEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHCEEEEEEEEE
HMYLRPGEKVIEQLGGLHHFAQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHLDGSK
EEEECCHHHHHHHHCCHHHHHHHCCCEEECCCCEEEEECCCCCEEECCCEEEEECCCCCC
HFFTPTKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANISLEYHKENNRP
EEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
NNNLFAIIQGGTHLKMRSLSVGLTHGGFDGYAIGGLAVGESTNEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEEEECCCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCTCYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLERYTMDRQ
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA