Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 109946977

GI number: 109946977

Start: 314696

End: 315946

Strand: Direct

Name: glyA

Synonym: Hac_0369

Alternate gene names: 109946977

Gene position: 314696-315946 (Clockwise)

Preceding gene: 109946976

Following gene: 109946978

Centisome position: 20.25

GC content: 40.93

Gene sequence:

>1251_bases
ATGGCTTATTTTTTAGAACAAAGCGATAGTGAAATTTTTGAGTTGATCTTTGAAGAGTTTAAGCGCCAAAATGAGCATTT
AGAAATGATAGCGAGCGAGAATTACACTTTTGCTAGCGTTATGGAAGCTATGGGAAGTATTTTAACGAATAAATACGCTG
AAGGCTATCCTAATAAGCGCTATTATGGAGGCTGTGAAGTGGTGGATAAAGTAGAAAGCTTGGCCATAGAAAGGGCTAAA
AAGCTTTTTAATTGCCAGTTTGCTAATGTGCAAGCGCATTCAGGCTCGCAAGCTAATAACGCTGTCTATCACGCCCTTTT
AAAGCCTTATGACAAGATTTTAGGCATGGATTTAAGCTGTGGGGGGCATTTAACGCATGGCGCTAAAGTGAGTTTGACCG
GTAAGCATTACCAGAGCTTTTCTTATGGCGTGGGTTTAGATGGCTATATTGATTATGGAGAAACGCTAAAAATCGCTCAA
AGCGTTAAACCTCAAATCATTGTGTGTGGGTTTTCAGCCTATCCAAGGGAGATTGATTTTAAGAAATTTAGAGAAATCGC
TGACGAAGTGGGGGCATTACTGTTAGGCGATATAGCCCATGTGGCAGGGCTTGTAGTCGCTAGTGAGCATGCCCATCCTT
TCCCACATTGCCATGTGGTTTCAAGCACCACTCACAAGACTTTAAGAGGGCCTAGAGGGGGGCTTATTTTAACCAATGAT
GAAGAAATAGCGGCTAAGATTGATAAAGCGATCTTTCCAGGGACTCAAGGCGGACCTTTGATGCATGCGATTGCTGCAAA
AGCGGTGGGGTTTAAAGAGAATTTAAAGCCAGAGTTTAAAACTTATGCAAAGTTGGTGAAATCTAACATGCAAGTTCTCG
CTAAAGTGTTAAAAGAAAAAAACCATAAGTTAGTGAGCGATGGCACTTCCAACCATTTGCTTTTAATGGATTTTTTAAAC
AAGCCTTATAGCGGAAAAGATGCTGACATTGCATTAGGGAATGCCGGAATCACTGTGAATAAAAACACCATTCCTGGTGA
AACGCGTAGCCCTTTTGTAACGAGCGGGATAAGGATTGGCTCAGCGGCATTGAGTGCAAGGGGCATGGGCACTAAGGAGT
TTGAAATCATAGGGAACAAAATATCAGATATTTTGAATGATATCAATAATGTTAGTTTGCAATTGCATGTGAAAGAAGAA
TTGAAAGCGATGGCTAGCCAATTCCCTGTGTATCACCAACCTATTTTTTAA

Upstream 100 bases:

>100_bases
TAGTGATGCTACTCACTGGGGCTAAAAGTATTAAAGATGTGATTTTATTCCCAGCGATGCGTCCTGTTAAAAACGATTTT
AATATTGAGAGTGAAGAATA

Downstream 100 bases:

>100_bases
GGAAGTCAAGATGACAGAAATGGAATTAAAACTTATTAAAATAGACACAAGCCATTATTTTGAAAAAAAACCAGGCTTGG
GGGAGAGGGTGGATTATGCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAYFLEQSDSEIFELIFEEFKRQNEHLEMIASENYTFASVMEAMGSILTNKYAEGYPNKRYYGGCEVVDKVESLAIERAK
KLFNCQFANVQAHSGSQANNAVYHALLKPYDKILGMDLSCGGHLTHGAKVSLTGKHYQSFSYGVGLDGYIDYGETLKIAQ
SVKPQIIVCGFSAYPREIDFKKFREIADEVGALLLGDIAHVAGLVVASEHAHPFPHCHVVSSTTHKTLRGPRGGLILTND
EEIAAKIDKAIFPGTQGGPLMHAIAAKAVGFKENLKPEFKTYAKLVKSNMQVLAKVLKEKNHKLVSDGTSNHLLLMDFLN
KPYSGKDADIALGNAGITVNKNTIPGETRSPFVTSGIRIGSAALSARGMGTKEFEIIGNKISDILNDINNVSLQLHVKEE
LKAMASQFPVYHQPIF

Sequences:

>Translated_416_residues
MAYFLEQSDSEIFELIFEEFKRQNEHLEMIASENYTFASVMEAMGSILTNKYAEGYPNKRYYGGCEVVDKVESLAIERAK
KLFNCQFANVQAHSGSQANNAVYHALLKPYDKILGMDLSCGGHLTHGAKVSLTGKHYQSFSYGVGLDGYIDYGETLKIAQ
SVKPQIIVCGFSAYPREIDFKKFREIADEVGALLLGDIAHVAGLVVASEHAHPFPHCHVVSSTTHKTLRGPRGGLILTND
EEIAAKIDKAIFPGTQGGPLMHAIAAKAVGFKENLKPEFKTYAKLVKSNMQVLAKVLKEKNHKLVSDGTSNHLLLMDFLN
KPYSGKDADIALGNAGITVNKNTIPGETRSPFVTSGIRIGSAALSARGMGTKEFEIIGNKISDILNDINNVSLQLHVKEE
LKAMASQFPVYHQPIF
>Mature_415_residues
AYFLEQSDSEIFELIFEEFKRQNEHLEMIASENYTFASVMEAMGSILTNKYAEGYPNKRYYGGCEVVDKVESLAIERAKK
LFNCQFANVQAHSGSQANNAVYHALLKPYDKILGMDLSCGGHLTHGAKVSLTGKHYQSFSYGVGLDGYIDYGETLKIAQS
VKPQIIVCGFSAYPREIDFKKFREIADEVGALLLGDIAHVAGLVVASEHAHPFPHCHVVSSTTHKTLRGPRGGLILTNDE
EIAAKIDKAIFPGTQGGPLMHAIAAKAVGFKENLKPEFKTYAKLVKSNMQVLAKVLKEKNHKLVSDGTSNHLLLMDFLNK
PYSGKDADIALGNAGITVNKNTIPGETRSPFVTSGIRIGSAALSARGMGTKEFEIIGNKISDILNDINNVSLQLHVKEEL
KAMASQFPVYHQPIF

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=418, Percent_Identity=43.3014354066986, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI261862352, Length=418, Percent_Identity=43.3014354066986, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI261862350, Length=418, Percent_Identity=43.3014354066986, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI261862348, Length=418, Percent_Identity=43.3014354066986, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI261862346, Length=417, Percent_Identity=42.6858513189448, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI22547186, Length=432, Percent_Identity=41.6666666666667, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI22547189, Length=418, Percent_Identity=39.7129186602871, Blast_Score=276, Evalue=4e-74,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=53.8271604938272, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=43, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=43, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6319739, Length=429, Percent_Identity=38.9277389277389, Blast_Score=306, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=41.0579345088161, Blast_Score=297, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640005, Length=396, Percent_Identity=43.6868686868687, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI221329721, Length=396, Percent_Identity=43.6868686868687, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_HELAH (Q17YS0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664205.1
- ProteinModelPortal:   Q17YS0
- SMR:   Q17YS0
- STRING:   Q17YS0
- GeneID:   4177263
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0369
- NMPDR:   fig|382638.8.peg.357
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   DICADIR
- ProtClustDB:   PRK13034
- BioCyc:   HACI382638:HAC_0369-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45643; Mature: 45512

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYFLEQSDSEIFELIFEEFKRQNEHLEMIASENYTFASVMEAMGSILTNKYAEGYPNKR
CCEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YYGGCEVVDKVESLAIERAKKLFNCQFANVQAHSGSQANNAVYHALLKPYDKILGMDLSC
CCCCHHHHHHHHHHHHHHHHHHHCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GGHLTHGAKVSLTGKHYQSFSYGVGLDGYIDYGETLKIAQSVKPQIIVCGFSAYPREIDF
CCCCCCCCEEEEECCHHCCCCCCCCCCCEECCCHHHHHHHCCCCEEEEEECCCCCCCCCH
KKFREIADEVGALLLGDIAHVAGLVVASEHAHPFPHCHVVSSTTHKTLRGPRGGLILTND
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCCEEEECC
EEIAAKIDKAIFPGTQGGPLMHAIAAKAVGFKENLKPEFKTYAKLVKSNMQVLAKVLKEK
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NHKLVSDGTSNHLLLMDFLNKPYSGKDADIALGNAGITVNKNTIPGETRSPFVTSGIRIG
CCEEECCCCCCCEEEEHHHCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEECCCEEEC
SAALSARGMGTKEFEIIGNKISDILNDINNVSLQLHVKEELKAMASQFPVYHQPIF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYFLEQSDSEIFELIFEEFKRQNEHLEMIASENYTFASVMEAMGSILTNKYAEGYPNKR
CEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YYGGCEVVDKVESLAIERAKKLFNCQFANVQAHSGSQANNAVYHALLKPYDKILGMDLSC
CCCCHHHHHHHHHHHHHHHHHHHCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GGHLTHGAKVSLTGKHYQSFSYGVGLDGYIDYGETLKIAQSVKPQIIVCGFSAYPREIDF
CCCCCCCCEEEEECCHHCCCCCCCCCCCEECCCHHHHHHHCCCCEEEEEECCCCCCCCCH
KKFREIADEVGALLLGDIAHVAGLVVASEHAHPFPHCHVVSSTTHKTLRGPRGGLILTND
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCCEEEECC
EEIAAKIDKAIFPGTQGGPLMHAIAAKAVGFKENLKPEFKTYAKLVKSNMQVLAKVLKEK
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NHKLVSDGTSNHLLLMDFLNKPYSGKDADIALGNAGITVNKNTIPGETRSPFVTSGIRIG
CCEEECCCCCCCEEEEHHHCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEECCCEEEC
SAALSARGMGTKEFEIIGNKISDILNDINNVSLQLHVKEELKAMASQFPVYHQPIF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA