Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 109946976

GI number: 109946976

Start: 313191

End: 314696

Strand: Direct

Name: lysS

Synonym: Hac_0368

Alternate gene names: 109946976

Gene position: 313191-314696 (Clockwise)

Preceding gene: 109946975

Following gene: 109946977

Centisome position: 20.15

GC content: 38.38

Gene sequence:

>1506_bases
ATGTTTTCTAACCAATACATCCAACAACGCATTCATAAAGCCAACAGCTTGAGAGAAGAAGGGAAAAACCCTTATAAAAA
TGGCTTGAAACGAAGCCTAACAAACGCCGCTTTTTTAGAAAAATACGCTTATGTTAAGGATTTAGAAGGGCCTAAAGACA
AAGAAAAATGCGAGAGTATTGTAGGGAGGGTCAAGCTCTTGCGTTTAATGGGTAAGGCATGTTTTATTAAAATTGAAGAT
GAAAGTGCGATTTTACAAGCCTATGTTTCACAAAATGAATTGAACGATGAATTTAAAAGCCTAAAAAAGCATTTAGAAGT
GGGCGATATTGTGTTGGTGAAAGGCTTTCCTTTTGCCACTAAAACCGGTGAATTAAGCATTCATGCCCTAGAATTCCACA
TTTTAAGCAAAACCATTGTCCCCTTACCTGAAAAGTTTCATGGATTAAGCGATATAGAATTGCGTTACCGCCAGCGTTAC
TTGGATTTGATAGTCAATCCTAGCGTTAAAGATGTGTTTAAAAAGCGCAGTTTGATTGTCTCTAGCGTGCGTAAATTCTT
TGAAATGGAAGGCTTTTTGGAAGTAGAAACTCCGATGATGCACCCCATTCCTGGCGGGGCGAACGCAAGGCCTTTTATCA
CTTATCATAACGCTTTGGAAATTGAAAGGTATTTAAGGATTGCCCCAGAATTATACCTTAAACGCTTGATTGTAGGGGGT
TTTGAAGCGGTGTTTGAAATCAATCGTAATTTTAGGAATGAAGGCATGGATCATAGCCATAACCCCGAATTTACGATGAT
TGAGTTTTATTGGGCGTATCACACTTATGAAGATTTGATTGAACTAAGCAAGAGGTTATTTGACTACTTGCTAAAGACTT
TAAATTTAGATTCAAAAATCATTTATAACGACATGGAAGTGGATTTCAATCAAACGAGCGTGATTTCCTATTTAGACGCT
TTAGAAACAATAGGGGGCATTAGCAAGGAGATTCTAGAAAAAGAAGACAAACTTTTAGCTTATTTGTTAGAGCAAGGTAT
CAAAGTAGAGCCTAATCTCACTCATGGTAAATTACTCGCTGAAGCGTTTGATCGTTTTGTAGAACACCAACTTATTCACC
CCACTTTTGTAACCGAATACCCTATTGAGATTAGCCCGCTAGCCAGACGCAACGATAGTAACCCTAATATTGCTGACAGG
TTTGAATTGTTTATTGCAGGAAAAGAAATCGCTAACGGCTTTAGCGAATTGAATGACCCCTTAGATCAATTAGAACGCTT
TAAAAATCAAGTAGCCGAAAAAGAAAAAGGCGATGAAGAAGCCCAATACATGGACGAAGATTATGTGTGGGCGTTAGCTC
ATGGAATGCCCCCCACTGCAGGGCAAGGCATAGGCATTGACAGATTAGTGATGCTACTCACTGGGGCTAAAAGTATTAAA
GATGTGATTTTATTCCCAGCGATGCGTCCTGTTAAAAACGATTTTAATATTGAGAGTGAAGAATAA

Upstream 100 bases:

>100_bases
AGAAAAGGCTAAAGATTCGCCTAAAAACATGCTAGAGCTAAAAGATAACCTAATTAAGCCCAACCAAAACCCATCTAAAG
AACCCCTATAAAGGCAAAAC

Downstream 100 bases:

>100_bases
TGGCTTATTTTTTAGAACAAAGCGATAGTGAAATTTTTGAGTTGATCTTTGAAGAGTTTAAGCGCCAAAATGAGCATTTA
GAAATGATAGCGAGCGAGAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKDLEGPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSIHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFEMEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLDSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKEILEKEDKLLAYLLEQGIKVEPNLTHGKLLAEAFDRFVEHQLIHPTFVTEYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNIESEE

Sequences:

>Translated_501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKDLEGPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSIHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFEMEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLDSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKEILEKEDKLLAYLLEQGIKVEPNLTHGKLLAEAFDRFVEHQLIHPTFVTEYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNIESEE
>Mature_501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKDLEGPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSIHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFEMEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLDSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKEILEKEDKLLAYLLEQGIKVEPNLTHGKLLAEAFDRFVEHQLIHPTFVTEYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNIESEE

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=515, Percent_Identity=39.6116504854369, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI5031815, Length=515, Percent_Identity=39.6116504854369, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI45439306, Length=462, Percent_Identity=24.025974025974, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=50.7936507936508, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1790571, Length=504, Percent_Identity=50, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI87082379, Length=333, Percent_Identity=32.1321321321321, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788173, Length=246, Percent_Identity=28.4552845528455, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=507, Percent_Identity=40.4339250493097, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=507, Percent_Identity=40.4339250493097, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=502, Percent_Identity=40.6374501992032, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17551876, Length=469, Percent_Identity=22.8144989339019, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=266, Percent_Identity=25.5639097744361, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=514, Percent_Identity=38.5214007782101, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6324256, Length=478, Percent_Identity=30.9623430962343, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=485, Percent_Identity=25.360824742268, Blast_Score=113, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24640849, Length=509, Percent_Identity=40.0785854616896, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24640851, Length=509, Percent_Identity=40.0785854616896, Blast_Score=358, Evalue=6e-99,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_HELAH (Q17YS1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664204.1
- ProteinModelPortal:   Q17YS1
- SMR:   Q17YS1
- STRING:   Q17YS1
- GeneID:   4177630
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0368
- NMPDR:   fig|382638.8.peg.356
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- PhylomeDB:   Q17YS1
- ProtClustDB:   PRK00484
- BioCyc:   HACI382638:HAC_0368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57756; Mature: 57756

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKDLEGPKDKEKCESI
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VGRVKLLRLMGKACFIKIEDESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFAT
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
KTGELSIHALEFHILSKTIVPLPEKFHGLSDIELRYRQRYLDLIVNPSVKDVFKKRSLIV
CCCCEEEEEEHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SSVRKFFEMEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLDSKI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYNDMEVDFNQTSVISYLDALETIGGISKEILEKEDKLLAYLLEQGIKVEPNLTHGKLLA
EEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EAFDRFVEHQLIHPTFVTEYPIEISPLARRNDSNPNIADRFELFIAGKEIANGFSELNDP
HHHHHHHHHHHCCCCEEECCCCEECCHHHCCCCCCCHHHHHHHEEECHHHHHHHHHHCCH
LDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHH
DVILFPAMRPVKNDFNIESEE
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKDLEGPKDKEKCESI
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VGRVKLLRLMGKACFIKIEDESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFAT
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
KTGELSIHALEFHILSKTIVPLPEKFHGLSDIELRYRQRYLDLIVNPSVKDVFKKRSLIV
CCCCEEEEEEHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SSVRKFFEMEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLDSKI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYNDMEVDFNQTSVISYLDALETIGGISKEILEKEDKLLAYLLEQGIKVEPNLTHGKLLA
EEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EAFDRFVEHQLIHPTFVTEYPIEISPLARRNDSNPNIADRFELFIAGKEIANGFSELNDP
HHHHHHHHHHHCCCCEEECCCCEECCHHHCCCCCCCHHHHHHHEEECHHHHHHHHHHCCH
LDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHH
DVILFPAMRPVKNDFNIESEE
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA