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The map label for this gene is hphIAM [H]

Identifier: 109946968

GI number: 109946968

Start: 304795

End: 305928

Strand: Direct

Name: hphIAM [H]

Synonym: Hac_0360

Alternate gene names: 109946968

Gene position: 304795-305928 (Clockwise)

Preceding gene: 109946967

Following gene: 109946969

Centisome position: 19.61

GC content: 31.92

Gene sequence:

>1134_bases
ATGGTTATGACATTAAGAAATGCTTTGATTAAACGCTTTAAAATTAGTGAAAAAGAAACAAAAAAAAGAGTTATCAAATT
TGATATTCAAAAAACTGATGAATTGATCAATGGCTCTTGGAGTGATGAAACTCTTAAAATTTATGGCTCTGATAACGACG
AAAATGTTAGCCAATTTGGATTAAATGGTGTCGTTAATGGTAGAAAAATAGATGTTATCTTTGGCGGTCCTCCTTGTCAA
GCCTATTCTCTTGCAGGTAGGGCGCAAGATAAACACTCTATGAAATATGATTATAGGAATTATCTTTTTGAAAGTTTTGT
TAAGATAGTTGATTATTATCAACCAAAATGCTTTGTGTTTGAAAATGTTCCTGGCATGCTTAGTGCAAAACCTGGTGATC
AGTTCGTGAAAGATAGGATTTATGAAGCTTTTTTAAAAATAGGGTATGAAATTAAAAAACCAAATGAAATGAAAGATATA
ATGTATTCTAGTGGTGATTATGAAGTCCCACAAACTAGAAAACGAGTAATCATATTTGGTGTTCGTAAAAACAACAAAGA
ATTACTATCTAAATTTTATCAAAACCTAGACGATCTCAAATCAAAAAACCCACCGCTTAGCGTTAAAGACGCTATTGGCC
ATTTACCCAAGTTTAGACCACTCAAAACACCTCTAAAAATCAACAATAAAAACATTTCTCACGAACTTATCGGCACAAAT
AATTTGACACAAAATTTTCCACGATATAATAATCTACGAGATTTAAAAGCGATGAAATTTTGGATTGAAAACAACATGAA
TAACGCTTCCACAAAAGAAAAGCTTGATTTTTATACTAGGATTACTGGAAAAGTTTCAAACCATAACAAATATAGAAATT
TAGAATGGGATAAACCATCGCCAACCTTAGTGGCGCATTTACAAAAAGATGGTTTTATGTTTATACATCCAGAAGCCGAT
CAAAGCCGTTCAATCACTATAAGAGAAGCAGCGATTTTACAGACTTTCCCTAATGATTTTGAATTTATGGGATCACAAGT
GGCTTGTTTTAAAATGATAGGCAATGCAGTGCCAGTCAATTTTGCCAAAAATATAGCTTTAGCAGTAGCAAAGGTTTTAG
ATGAAAAAAACTAA

Upstream 100 bases:

>100_bases
TAATTGCATAGATTTATTTAGTGGAGCTGGCGGTTTATCATTGGGTTTTGCAAATACAAATAGATTTAATATTTTAGCCC
ACATTGAGTGGGAAAAACCT

Downstream 100 bases:

>100_bases
TATAAATGTTTTAATAGCTTGTGAAGAAAGCCAAAGAGTGTGTAATGAGTTTAGAAAGCTTGGTTTCAACGCTTATAGTT
GTGATTTGTTAGAGTGTAGT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MVMTLRNALIKRFKISEKETKKRVIKFDIQKTDELINGSWSDETLKIYGSDNDENVSQFGLNGVVNGRKIDVIFGGPPCQ
AYSLAGRAQDKHSMKYDYRNYLFESFVKIVDYYQPKCFVFENVPGMLSAKPGDQFVKDRIYEAFLKIGYEIKKPNEMKDI
MYSSGDYEVPQTRKRVIIFGVRKNNKELLSKFYQNLDDLKSKNPPLSVKDAIGHLPKFRPLKTPLKINNKNISHELIGTN
NLTQNFPRYNNLRDLKAMKFWIENNMNNASTKEKLDFYTRITGKVSNHNKYRNLEWDKPSPTLVAHLQKDGFMFIHPEAD
QSRSITIREAAILQTFPNDFEFMGSQVACFKMIGNAVPVNFAKNIALAVAKVLDEKN

Sequences:

>Translated_377_residues
MVMTLRNALIKRFKISEKETKKRVIKFDIQKTDELINGSWSDETLKIYGSDNDENVSQFGLNGVVNGRKIDVIFGGPPCQ
AYSLAGRAQDKHSMKYDYRNYLFESFVKIVDYYQPKCFVFENVPGMLSAKPGDQFVKDRIYEAFLKIGYEIKKPNEMKDI
MYSSGDYEVPQTRKRVIIFGVRKNNKELLSKFYQNLDDLKSKNPPLSVKDAIGHLPKFRPLKTPLKINNKNISHELIGTN
NLTQNFPRYNNLRDLKAMKFWIENNMNNASTKEKLDFYTRITGKVSNHNKYRNLEWDKPSPTLVAHLQKDGFMFIHPEAD
QSRSITIREAAILQTFPNDFEFMGSQVACFKMIGNAVPVNFAKNIALAVAKVLDEKN
>Mature_377_residues
MVMTLRNALIKRFKISEKETKKRVIKFDIQKTDELINGSWSDETLKIYGSDNDENVSQFGLNGVVNGRKIDVIFGGPPCQ
AYSLAGRAQDKHSMKYDYRNYLFESFVKIVDYYQPKCFVFENVPGMLSAKPGDQFVKDRIYEAFLKIGYEIKKPNEMKDI
MYSSGDYEVPQTRKRVIIFGVRKNNKELLSKFYQNLDDLKSKNPPLSVKDAIGHLPKFRPLKTPLKINNKNISHELIGTN
NLTQNFPRYNNLRDLKAMKFWIENNMNNASTKEKLDFYTRITGKVSNHNKYRNLEWDKPSPTLVAHLQKDGFMFIHPEAD
QSRSITIREAAILQTFPNDFEFMGSQVACFKMIGNAVPVNFAKNIALAVAKVLDEKN

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 43516; Mature: 43516

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMTLRNALIKRFKISEKETKKRVIKFDIQKTDELINGSWSDETLKIYGSDNDENVSQFG
CEEEHHHHHHHHHCCCHHHHHHHHEEEEHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHC
LNGVVNGRKIDVIFGGPPCQAYSLAGRAQDKHSMKYDYRNYLFESFVKIVDYYQPKCFVF
CCCEECCEEEEEEECCCCCCHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ENVPGMLSAKPGDQFVKDRIYEAFLKIGYEIKKPNEMKDIMYSSGDYEVPQTRKRVIIFG
ECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCCCCCCCCCCEEEEEE
VRKNNKELLSKFYQNLDDLKSKNPPLSVKDAIGHLPKFRPLKTPLKINNKNISHELIGTN
ECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCC
NLTQNFPRYNNLRDLKAMKFWIENNMNNASTKEKLDFYTRITGKVSNHNKYRNLEWDKPS
CCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECEECCCCCCCCCCCCCCC
PTLVAHLQKDGFMFIHPEADQSRSITIREAAILQTFPNDFEFMGSQVACFKMIGNAVPVN
CCEEEEECCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCCH
FAKNIALAVAKVLDEKN
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVMTLRNALIKRFKISEKETKKRVIKFDIQKTDELINGSWSDETLKIYGSDNDENVSQFG
CEEEHHHHHHHHHCCCHHHHHHHHEEEEHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHC
LNGVVNGRKIDVIFGGPPCQAYSLAGRAQDKHSMKYDYRNYLFESFVKIVDYYQPKCFVF
CCCEECCEEEEEEECCCCCCHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ENVPGMLSAKPGDQFVKDRIYEAFLKIGYEIKKPNEMKDIMYSSGDYEVPQTRKRVIIFG
ECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCCCCCCCCCCEEEEEE
VRKNNKELLSKFYQNLDDLKSKNPPLSVKDAIGHLPKFRPLKTPLKINNKNISHELIGTN
ECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCC
NLTQNFPRYNNLRDLKAMKFWIENNMNNASTKEKLDFYTRITGKVSNHNKYRNLEWDKPS
CCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECEECCCCCCCCCCCCCCC
PTLVAHLQKDGFMFIHPEADQSRSITIREAAILQTFPNDFEFMGSQVACFKMIGNAVPVN
CCEEEEECCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCCH
FAKNIALAVAKVLDEKN
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]