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The map label for this gene is eno

Identifier: 109946947

GI number: 109946947

Start: 285672

End: 286952

Strand: Direct

Name: eno

Synonym: Hac_0337

Alternate gene names: 109946947

Gene position: 285672-286952 (Clockwise)

Preceding gene: 109946946

Following gene: 109946948

Centisome position: 18.38

GC content: 42.39

Gene sequence:

>1281_bases
ATGCTAACGATTAAAGATATTCATGCTTTAGAAGTGATGGATAGTAGGGGTAATCCCACCATTCAAGCCAGCGTGATTTT
AAGCGATAACACGAAAGCGAGCGCGATTGTGTCTAGTGGGGCGAGCACCGGTAAAAGAGAAGCACTAGAATTAAGAGATA
ACGACAAAACCCGTTTTTTAGGTAAGGGGGTCTTAAGGGCATGCGAAAATGTCAATACTGTGATCAAACACAACTTAATA
GGGCTTGAAGCGATCAATCAAGCCTTTGTGGATGGGAGACTGAAGGCTTTAGATGGCACACCTAATTACGCTAATTTAGG
GGCGAATGCCTCCTTGGGCGTGTCTATGGCGTTAGCAAGAGCGAGTGCAAAGGCTTTAAACATGCCATTATATCGCTATT
TAGGGGGGGCTAACGCTTTAACTTTGCCTGTTCCGATGCTCAATATCATCAACGGCGGATCGCATGCCAATAATTCTATA
GACTTCCAAGAATACATGATCATGCCTTTAGGGTTTGAGAGCTTTAAAGAAGCCTTAAGAGCGAGTGCAGAAGTCTATCA
CACGCTCAAAAAACTTTTGGATGAAAAAAACCAGCTCACAAGCGTGGGCGATGAAGGGGGCTTTGCACCTAATTTTAAAA
ACAATGTAGAACCCCTTGAAGTGATTTCTCAAGCCATTGAAAAAGCCGGTTACAAGCTAGGCGAAGAAATCGCACTCGCT
TTAGATGTGGCGAGCAGCGAATTGGTGGATGAACATTTTAATTACCATTTAAAAGGTGAAAATAAGATTTTAAACGCACA
AGAATTGGTGGCTTATTATAAAGAGTTGGTCGCAAAATACCCAATTGTGTCCATTGAAGATGGTTTGAGCGAAGACGATT
GGGAGGGTTGGGCGTTTTTAAGCAAGGAATTAGGGCGTCAAATTCAGCTGGTGGGCGATGATCTGTTTGTAACGAATGCG
AGTATTTTGCAAAAGGGTATTAAAAAAAACATTGCCAATGCGATTTTAATCAAACCCAACCAAATTGGCACTATTAGCGA
GACTTTAGAGACCATAAGATTAGCCAAACACCATGCCTATCAATGCGTGATGAGCCATAGAAGCGGAGAGAGTGAAGACA
GCTTTATCGCTGATTTTGCAGTCGCGCTCAATACAGGAGAAATCAAAACCGGATCCACCGCAAGGAGTGAAAGGATCGCT
AAATACAACCGTCTTTTAGAGATTGAGCATGAATTAAAAGGGGGGATTTATATCGGTAAAGAGTTGTTTAAACATGGCTG
A

Upstream 100 bases:

>100_bases
AATGAAATCATTCTTAAGATTAAAGAGAGTATTGGCTCTAATGAAGAGATCATGCCTTTACCGGATGAGCCCTTAGAAGA
AATGGAATAAAAGGATCTTG

Downstream 100 bases:

>100_bases
GAGCCTTTTTGAAAACGATGGAATCAAAAGCAATAAAGCACGAGACTTTTTCTATAACCATAGCTCACTTATTGTTTTTT
TCCTTTTATTGCTTGGGTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVSSGASTGKREALELRDNDKTRFLGKGVLRACENVNTVIKHNLI
GLEAINQAFVDGRLKALDGTPNYANLGANASLGVSMALARASAKALNMPLYRYLGGANALTLPVPMLNIINGGSHANNSI
DFQEYMIMPLGFESFKEALRASAEVYHTLKKLLDEKNQLTSVGDEGGFAPNFKNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDEHFNYHLKGENKILNAQELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIKKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Sequences:

>Translated_426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVSSGASTGKREALELRDNDKTRFLGKGVLRACENVNTVIKHNLI
GLEAINQAFVDGRLKALDGTPNYANLGANASLGVSMALARASAKALNMPLYRYLGGANALTLPVPMLNIINGGSHANNSI
DFQEYMIMPLGFESFKEALRASAEVYHTLKKLLDEKNQLTSVGDEGGFAPNFKNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDEHFNYHLKGENKILNAQELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIKKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG
>Mature_426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVSSGASTGKREALELRDNDKTRFLGKGVLRACENVNTVIKHNLI
GLEAINQAFVDGRLKALDGTPNYANLGANASLGVSMALARASAKALNMPLYRYLGGANALTLPVPMLNIINGGSHANNSI
DFQEYMIMPLGFESFKEALRASAEVYHTLKKLLDEKNQLTSVGDEGGFAPNFKNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDEHFNYHLKGENKILNAQELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIKKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=422, Percent_Identity=47.1563981042654, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI4503571, Length=422, Percent_Identity=46.4454976303318, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=45.6221198156682, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=45.6221198156682, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=42.1296296296296, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=27.0588235294118, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=27.0588235294118, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=27.0588235294118, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=53.4772182254197, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=46.8677494199536, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=46.8677494199536, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=43.6842105263158, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=45.7858769931663, Blast_Score=349, Evalue=6e-97,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=42.7251732101617, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=42.7251732101617, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=42.7251732101617, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=45.7858769931663, Blast_Score=330, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=46.2068965517241, Blast_Score=357, Evalue=8e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_HELAH (Q17YV0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664175.1
- ProteinModelPortal:   Q17YV0
- SMR:   Q17YV0
- STRING:   Q17YV0
- GeneID:   4177398
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0337
- NMPDR:   fig|382638.8.peg.326
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   HACI382638:HAC_0337-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46545; Mature: 46545

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 338-338 BINDING 155-155 BINDING 164-164 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVSSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNTVIKHNLIGLEAINQAFVDGRLKALDGTPNYANLGANASLGVSMALAR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHH
ASAKALNMPLYRYLGGANALTLPVPMLNIINGGSHANNSIDFQEYMIMPLGFESFKEALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASAEVYHTLKKLLDEKNQLTSVGDEGGFAPNFKNNVEPLEVISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDEHFNYHLKGENKILNAQELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASILQKGIKKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC
>Mature Secondary Structure
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVSSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNTVIKHNLIGLEAINQAFVDGRLKALDGTPNYANLGANASLGVSMALAR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHH
ASAKALNMPLYRYLGGANALTLPVPMLNIINGGSHANNSIDFQEYMIMPLGFESFKEALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASAEVYHTLKKLLDEKNQLTSVGDEGGFAPNFKNNVEPLEVISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDEHFNYHLKGENKILNAQELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASILQKGIKKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA