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The map label for this gene is citT [H]

Identifier: 109946936

GI number: 109946936

Start: 276101

End: 277549

Strand: Reverse

Name: citT [H]

Synonym: Hac_0324

Alternate gene names: 109946936

Gene position: 277549-276101 (Counterclockwise)

Preceding gene: 109946945

Following gene: 109946935

Centisome position: 17.86

GC content: 41.27

Gene sequence:

>1449_bases
ATGAAAAAACCAATCGTTTCAATCCTTGCCCCCTTTTTTATCGCAGTATTATTGTATTTTTTAGGCGCTCCTAGTGGGCT
AAATCCTAACGCATGGCTTTATTTTTGTGTTTTTATAGGCATGATTGTAGGGCTTATTTTAGAGCCGGTGCCAGCAGGCT
TGGTAGCGTTGAGCGCGTTAATGTTATGCGTGGCGTTAAAAATTGGCGTTAATAGTGAAATAGCGAGCGCTAATAAGGCT
ATTTCGTGGGGCTTGAGTGGGTATGCAAATAAAACGGTGTGGCTCGTGTTTGTCGCTTTTATTTTGGGTTTAGGGTATGA
AAAAAGCTTGTTAGGGAAACGGATCGCTCTTTTACTCATTAGATTTTTAGGGCAAACCCCTTTAGGTTTAGGCTATGCGG
TTAGTTTGAGCGAATTGTGTCTAGCCCCCTTTATCCCTAGCAACTCCGCTAGAAGTGGGGGTATACTCTACCCCATCGTC
TCTTCTATCCCGCCTCTAATGGGATCTACCCCCAATAATAACCCTAACAAAATTGGTGCGTATTTGATGTGGGTCGCATT
AGCTTCAACTTGCATCACTTCGTCCATGTTTTTAACCGCGCTCGCTCCTAATCCCCTAGCAATGGAAATCGCCACCAAAA
TGGGAGTGCGTGAAATCTCATGGTTTTCATGGTTTTTAGCGTTCTTGCCTTGTGGGGTGCTTTTAATCTTGCTTGTGCCT
TTATTGGCGTATAAAACCTGCACCCCCACCTTAAAAGGCTCAAAAGAAGTGAGTTTGTGGGCTAAAAAAGAATTAGAGGG
CATGGGGAGGTTTTCTTTAAAAGAAATTTTAATGCTCAGTCTCACTTTACTGGCTTTATTGGGTTGGATTTTTGGCAAAT
CTTTAGGCTTGCATGCGAGCGCGACGGCTTTGATTGTCATGGTTTTAATGGCTTTTTGTAAGATTGTAAGCTATGAAGAC
ACCATTAAAAATAAGAGTGCATTTAATATTTTTTTATTGCTTGGAGCGTTGCTCACGATGGCCGGTGGGCTTAAAAATGT
AGGCTTTTTAGATTTTATCGGTAATATTACTAGCGATTTTTTAAAGCATGCCAACTTGGATCCTTTAATAGCGTTATTAC
TCGTCATAACCCTCTTTTATTTATCGCATTATTTCTTCGCAAGCATCACCGCCCATGTGAGTGCATTATTAGCCCTTTTT
ATAGGGATAGGCTCGCATATTCAAGGGATCAATTTGCAAGAATTGAGTTTGTTTTTGATGTTATCTTTAGGGATCATGGG
GATTTTAACGCCCTATGGCACAGGCCCATCCACCATCTATTATGGGAGTGGGTATATTCAAAGCAAGGATTTTTGGAAAT
TGGGGCTTATTTTTGGGTTTGTGTATTTGATCGTGTTTTTAAGCGTGTGCGTGCCTTGGGTAAAATCCATCGCTTTTAGG
TGGTTATAG

Upstream 100 bases:

>100_bases
TGTAACATTAAATTAAGAGTTAAATGCAATTTAATTTGATGATTTGAGTGCGATTTTTAAATTGAGTGCACCCCTTGTCT
TTTTTAATGAAAGGATAAAC

Downstream 100 bases:

>100_bases
CTGGAAACTTTACACAACGCCCTTTTAAAATGGTATGAAGAATTTGGGCGCAAGGATTTACCCTTTAGGAATTTAAAGGG
CATTAACGCCCCTTATGAAG

Product: citrate carrier

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKKPIVSILAPFFIAVLLYFLGAPSGLNPNAWLYFCVFIGMIVGLILEPVPAGLVALSALMLCVALKIGVNSEIASANKA
ISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAVSLSELCLAPFIPSNSARSGGILYPIV
SSIPPLMGSTPNNNPNKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKMGVREISWFSWFLAFLPCGVLLILLVP
LLAYKTCTPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKSLGLHASATALIVMVLMAFCKIVSYED
TIKNKSAFNIFLLLGALLTMAGGLKNVGFLDFIGNITSDFLKHANLDPLIALLLVITLFYLSHYFFASITAHVSALLALF
IGIGSHIQGINLQELSLFLMLSLGIMGILTPYGTGPSTIYYGSGYIQSKDFWKLGLIFGFVYLIVFLSVCVPWVKSIAFR
WL

Sequences:

>Translated_482_residues
MKKPIVSILAPFFIAVLLYFLGAPSGLNPNAWLYFCVFIGMIVGLILEPVPAGLVALSALMLCVALKIGVNSEIASANKA
ISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAVSLSELCLAPFIPSNSARSGGILYPIV
SSIPPLMGSTPNNNPNKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKMGVREISWFSWFLAFLPCGVLLILLVP
LLAYKTCTPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKSLGLHASATALIVMVLMAFCKIVSYED
TIKNKSAFNIFLLLGALLTMAGGLKNVGFLDFIGNITSDFLKHANLDPLIALLLVITLFYLSHYFFASITAHVSALLALF
IGIGSHIQGINLQELSLFLMLSLGIMGILTPYGTGPSTIYYGSGYIQSKDFWKLGLIFGFVYLIVFLSVCVPWVKSIAFR
WL
>Mature_482_residues
MKKPIVSILAPFFIAVLLYFLGAPSGLNPNAWLYFCVFIGMIVGLILEPVPAGLVALSALMLCVALKIGVNSEIASANKA
ISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLIRFLGQTPLGLGYAVSLSELCLAPFIPSNSARSGGILYPIV
SSIPPLMGSTPNNNPNKIGAYLMWVALASTCITSSMFLTALAPNPLAMEIATKMGVREISWFSWFLAFLPCGVLLILLVP
LLAYKTCTPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKSLGLHASATALIVMVLMAFCKIVSYED
TIKNKSAFNIFLLLGALLTMAGGLKNVGFLDFIGNITSDFLKHANLDPLIALLLVITLFYLSHYFFASITAHVSALLALF
IGIGSHIQGINLQELSLFLMLSLGIMGILTPYGTGPSTIYYGSGYIQSKDFWKLGLIFGFVYLIVFLSVCVPWVKSIAFR
WL

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=483, Percent_Identity=42.0289855072464, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1789444, Length=478, Percent_Identity=42.0502092050209, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1786986, Length=475, Percent_Identity=35.1578947368421, Blast_Score=261, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 52216; Mature: 52216

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIVSILAPFFIAVLLYFLGAPSGLNPNAWLYFCVFIGMIVGLILEPVPAGLVALSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLCVALKIGVNSEIASANKAISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLI
HHHHHHHHCCCHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFLGQTPLGLGYAVSLSELCLAPFIPSNSARSGGILYPIVSSIPPLMGSTPNNNPNKIGA
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCHHHHH
YLMWVALASTCITSSMFLTALAPNPLAMEIATKMGVREISWFSWFLAFLPCGVLLILLVP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LLAYKTCTPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKSLGLHAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ATALIVMVLMAFCKIVSYEDTIKNKSAFNIFLLLGALLTMAGGLKNVGFLDFIGNITSDF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKHANLDPLIALLLVITLFYLSHYFFASITAHVSALLALFIGIGSHIQGINLQELSLFLM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LSLGIMGILTPYGTGPSTIYYGSGYIQSKDFWKLGLIFGFVYLIVFLSVCVPWVKSIAFR
HHHHHHHHHCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WL
CC
>Mature Secondary Structure
MKKPIVSILAPFFIAVLLYFLGAPSGLNPNAWLYFCVFIGMIVGLILEPVPAGLVALSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLCVALKIGVNSEIASANKAISWGLSGYANKTVWLVFVAFILGLGYEKSLLGKRIALLLI
HHHHHHHHCCCHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFLGQTPLGLGYAVSLSELCLAPFIPSNSARSGGILYPIVSSIPPLMGSTPNNNPNKIGA
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCHHHHH
YLMWVALASTCITSSMFLTALAPNPLAMEIATKMGVREISWFSWFLAFLPCGVLLILLVP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LLAYKTCTPTLKGSKEVSLWAKKELEGMGRFSLKEILMLSLTLLALLGWIFGKSLGLHAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ATALIVMVLMAFCKIVSYEDTIKNKSAFNIFLLLGALLTMAGGLKNVGFLDFIGNITSDF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKHANLDPLIALLLVITLFYLSHYFFASITAHVSALLALFIGIGSHIQGINLQELSLFLM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LSLGIMGILTPYGTGPSTIYYGSGYIQSKDFWKLGLIFGFVYLIVFLSVCVPWVKSIAFR
HHHHHHHHHCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]