Click here to switch to the map view.

The map label for this gene is vacB [H]

Identifier: 109946864

GI number: 109946864

Start: 203963

End: 205897

Strand: Direct

Name: vacB [H]

Synonym: Hac_0245

Alternate gene names: 109946864

Gene position: 203963-205897 (Clockwise)

Preceding gene: 109946863

Following gene: 109946865

Centisome position: 13.13

GC content: 36.43

Gene sequence:

>1935_bases
ATGCAAGGGTTTTTAAGAAGCTTGTTTTTTGGGGTTAAAAAGATCCCTAAACCATTCGCCCCCTTAATAAGAAAGGGCGT
TTTAAAAGAAACGCTTGAATTGAAAAAGGAGCGTTGGATTCTAAAAGAAGGCTTTGATATAGGCAAGATTGAATGGGTAA
AAAATAAAGCGTTTTTTATTTCTTTAGCGAGAAATTACCCTAAAGACCCTTTAATTAAAAACTTACCCTCTTCTTTCAAA
ACAGATGCATTGATTTTATGCCAAATAGAATATTCTAAAAAACGCCCCATAGCCTTTTTTAAAGCCGCTCTTCTAAATGA
AAGCCATACAATGATAGCTTACTTAACAAAAGAAAATAACCAAATTGTGGCTATCCCTTTTAAAGAGCCTTTTAAGAAAG
CCCTTATCTTAAAACACAGCCAAAAATCCTTATTAGAATTGCCTAGGCATTGCGTGGTGAAAGTGGATATTAAAAAGCGT
GAGATCAGCGAGATTTTAGGGGCTTTAGAAGATCCTTTAATAGATGAAAACCTTTCTTTAAATCTTTTTGATAGAGTGAA
AGATTTTTCAAAAGATTGTTTGGGTTTAGCCAAACGCTATGCGCAACTTAAAGCGAGCGATTTTAAATGCAGAGTGGATT
ATTCTCATATCCCTTTTATCACCATTGACCCCAAAGACGCTAAAGATTTTGACGATGCGATTTTTTATGATCAAGAAAAA
AAAGTTTTGTTTGTGGCGGTTGCTGATGTGAGCGAATTCGTGCCAAAATATTCCAGTTTGGATAAAGAAGCTAGGATTAG
AGGCTTTAGCGTGTATTTTCCTAACAGCGTGTATCCCATGTTGCCTTTGAGTTTGTCTCAAGGGGCATGCTCGCTAAAGG
CGTTTGAAAAACGCTTAGCTTTGGTGTATGAAATCCCTTTAGATGATTTAAAAAACGTCCAATTGTTTCAAGGCGTTATT
GAAGTTAGGGCTAATTACAGTTATGAAGAAATCAATCATTTTTTAAGCACCCAACAAAGCTCTTTGAGTAAAGATTTACA
ACAAAGCCTTTTGGGGTTTTTAGAAATGGCTTTGAAGTTAAAAAAGGAGCGTTTGAAAAAGGGGTTTAATTTCAATTCGT
TTGAAAACAAGCTTTATTTAAACCAAGAAGGGCGTATAGAAAAAATTGAAACACAACAAGAAAGCGATGCACACACCCTT
ATAGAAGAAGCCATGCTCTTAGCCAATCAGTCTAGCGCTAGGTTATTGGATAAGCATTTTCAAAATAGGGGGATATACCG
CACCCATAAAGAGCCAAGTTTGGAGCAACAAAAACGCCTCTACGCCAAGCTTTTTGATTATGAGATCACACGCCCTAAAA
ACATGGGCTTTTTCCCTTTTTTAGAGCATGCCTTAAAAATAGCCAAAGAAAAGAATGTAGAAAGAGAAGTTTCGCGCTTA
ATGATTAAATCTCAAAATTTAGCCCTTTATAGCCCCATGCAAGAAAACCATTTTGGTTTGGGGTTTGCTAGCTATACGCA
TTTCACTTCGCCCATTAGAAGATACAGCGATCTAGCCTTACACAGGCTTTTAAAAGAATTGTTGTTCCATCAAGCTAAAG
GCTGCTCGTATTTGTTAGAAGAAATGCCTGAATTATGCTCTGAATTGAACGCTTTACAAAAAAAGGTTGCTTTGATTGAA
AGGGATTTCATCAAGCGTAAATTCGCCCGCTTGGCTTTAGAGTATTTAGAAAAAGAGTTTTTGGGCGTTGTTTTAGAAAC
TAAAGATGAAGTAATCGTGGGGCTAAAAGAGTGGGTAGGGCTTAAGGTTTTAGTAAAAACGAACAAGATTTTTAAACCCT
TAGAAAAGGTGCGCGTTAAAATCACGCATGCTCATTTAATTTTAGGGCAAGTTAGGGGTGAAATCACAGAAAGGATTAAA
GAGCATGTATCGTAA

Upstream 100 bases:

>100_bases
AAGGCATGCTCATCTATCAAGCCTCTTTAAGTTTTGAAAAATTTAGCTCTTCTCAAATCTCTTATTCAAAAGCGTTTGAA
ATCATGCGAAGTGTTTTTTG

Downstream 100 bases:

>100_bases
AGATTTGGATCATTATTTAAAACAGCATCTCCCTAAAGCGGTGTTTTTGTATGGGGAGTTTGATTTTTTCATCCATTATT
ACACTCAAGCGATTAGCTCA

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MQGFLRSLFFGVKKIPKPFAPLIRKGVLKETLELKKERWILKEGFDIGKIEWVKNKAFFISLARNYPKDPLIKNLPSSFK
TDALILCQIEYSKKRPIAFFKAALLNESHTMIAYLTKENNQIVAIPFKEPFKKALILKHSQKSLLELPRHCVVKVDIKKR
EISEILGALEDPLIDENLSLNLFDRVKDFSKDCLGLAKRYAQLKASDFKCRVDYSHIPFITIDPKDAKDFDDAIFYDQEK
KVLFVAVADVSEFVPKYSSLDKEARIRGFSVYFPNSVYPMLPLSLSQGACSLKAFEKRLALVYEIPLDDLKNVQLFQGVI
EVRANYSYEEINHFLSTQQSSLSKDLQQSLLGFLEMALKLKKERLKKGFNFNSFENKLYLNQEGRIEKIETQQESDAHTL
IEEAMLLANQSSARLLDKHFQNRGIYRTHKEPSLEQQKRLYAKLFDYEITRPKNMGFFPFLEHALKIAKEKNVEREVSRL
MIKSQNLALYSPMQENHFGLGFASYTHFTSPIRRYSDLALHRLLKELLFHQAKGCSYLLEEMPELCSELNALQKKVALIE
RDFIKRKFARLALEYLEKEFLGVVLETKDEVIVGLKEWVGLKVLVKTNKIFKPLEKVRVKITHAHLILGQVRGEITERIK
EHVS

Sequences:

>Translated_644_residues
MQGFLRSLFFGVKKIPKPFAPLIRKGVLKETLELKKERWILKEGFDIGKIEWVKNKAFFISLARNYPKDPLIKNLPSSFK
TDALILCQIEYSKKRPIAFFKAALLNESHTMIAYLTKENNQIVAIPFKEPFKKALILKHSQKSLLELPRHCVVKVDIKKR
EISEILGALEDPLIDENLSLNLFDRVKDFSKDCLGLAKRYAQLKASDFKCRVDYSHIPFITIDPKDAKDFDDAIFYDQEK
KVLFVAVADVSEFVPKYSSLDKEARIRGFSVYFPNSVYPMLPLSLSQGACSLKAFEKRLALVYEIPLDDLKNVQLFQGVI
EVRANYSYEEINHFLSTQQSSLSKDLQQSLLGFLEMALKLKKERLKKGFNFNSFENKLYLNQEGRIEKIETQQESDAHTL
IEEAMLLANQSSARLLDKHFQNRGIYRTHKEPSLEQQKRLYAKLFDYEITRPKNMGFFPFLEHALKIAKEKNVEREVSRL
MIKSQNLALYSPMQENHFGLGFASYTHFTSPIRRYSDLALHRLLKELLFHQAKGCSYLLEEMPELCSELNALQKKVALIE
RDFIKRKFARLALEYLEKEFLGVVLETKDEVIVGLKEWVGLKVLVKTNKIFKPLEKVRVKITHAHLILGQVRGEITERIK
EHVS
>Mature_644_residues
MQGFLRSLFFGVKKIPKPFAPLIRKGVLKETLELKKERWILKEGFDIGKIEWVKNKAFFISLARNYPKDPLIKNLPSSFK
TDALILCQIEYSKKRPIAFFKAALLNESHTMIAYLTKENNQIVAIPFKEPFKKALILKHSQKSLLELPRHCVVKVDIKKR
EISEILGALEDPLIDENLSLNLFDRVKDFSKDCLGLAKRYAQLKASDFKCRVDYSHIPFITIDPKDAKDFDDAIFYDQEK
KVLFVAVADVSEFVPKYSSLDKEARIRGFSVYFPNSVYPMLPLSLSQGACSLKAFEKRLALVYEIPLDDLKNVQLFQGVI
EVRANYSYEEINHFLSTQQSSLSKDLQQSLLGFLEMALKLKKERLKKGFNFNSFENKLYLNQEGRIEKIETQQESDAHTL
IEEAMLLANQSSARLLDKHFQNRGIYRTHKEPSLEQQKRLYAKLFDYEITRPKNMGFFPFLEHALKIAKEKNVEREVSRL
MIKSQNLALYSPMQENHFGLGFASYTHFTSPIRRYSDLALHRLLKELLFHQAKGCSYLLEEMPELCSELNALQKKVALIE
RDFIKRKFARLALEYLEKEFLGVVLETKDEVIVGLKEWVGLKVLVKTNKIFKPLEKVRVKITHAHLILGQVRGEITERIK
EHVS

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=401, Percent_Identity=29.426433915212, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI190014623, Length=401, Percent_Identity=29.426433915212, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI134288890, Length=389, Percent_Identity=27.7634961439589, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI219521928, Length=373, Percent_Identity=28.1501340482574, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI19115966, Length=373, Percent_Identity=28.1501340482574, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI87082383, Length=397, Percent_Identity=36.0201511335013, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1787542, Length=332, Percent_Identity=28.6144578313253, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI212645896, Length=420, Percent_Identity=29.0476190476191, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17553506, Length=406, Percent_Identity=27.5862068965517, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324552, Length=417, Percent_Identity=29.0167865707434, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323943, Length=435, Percent_Identity=25.0574712643678, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24649634, Length=393, Percent_Identity=28.7531806615776, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922976, Length=359, Percent_Identity=28.4122562674095, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654597, Length=359, Percent_Identity=28.4122562674095, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654592, Length=359, Percent_Identity=28.4122562674095, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR011805 [H]

Pfam domain/function: PF00773 RNB [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 74755; Mature: 74755

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFLRSLFFGVKKIPKPFAPLIRKGVLKETLELKKERWILKEGFDIGKIEWVKNKAFFI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEE
SLARNYPKDPLIKNLPSSFKTDALILCQIEYSKKRPIAFFKAALLNESHTMIAYLTKENN
EEHHCCCCCCHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCC
QIVAIPFKEPFKKALILKHSQKSLLELPRHCVVKVDIKKREISEILGALEDPLIDENLSL
EEEEECCHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
NLFDRVKDFSKDCLGLAKRYAQLKASDFKCRVDYSHIPFITIDPKDAKDFDDAIFYDQEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCHHHEECCCC
KVLFVAVADVSEFVPKYSSLDKEARIRGFSVYFPNSVYPMLPLSLSQGACSLKAFEKRLA
CEEEEEEHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
LVYEIPLDDLKNVQLFQGVIEVRANYSYEEINHFLSTQQSSLSKDLQQSLLGFLEMALKL
EEEECCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKERLKKGFNFNSFENKLYLNQEGRIEKIETQQESDAHTLIEEAMLLANQSSARLLDKHF
HHHHHHCCCCCCCCCCEEEECCCCCEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
QNRGIYRTHKEPSLEQQKRLYAKLFDYEITRPKNMGFFPFLEHALKIAKEKNVEREVSRL
HCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
MIKSQNLALYSPMQENHFGLGFASYTHFTSPIRRYSDLALHRLLKELLFHQAKGCSYLLE
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EMPELCSELNALQKKVALIERDFIKRKFARLALEYLEKEFLGVVLETKDEVIVGLKEWVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCC
LKVLVKTNKIFKPLEKVRVKITHAHLILGQVRGEITERIKEHVS
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGFLRSLFFGVKKIPKPFAPLIRKGVLKETLELKKERWILKEGFDIGKIEWVKNKAFFI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEE
SLARNYPKDPLIKNLPSSFKTDALILCQIEYSKKRPIAFFKAALLNESHTMIAYLTKENN
EEHHCCCCCCHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCC
QIVAIPFKEPFKKALILKHSQKSLLELPRHCVVKVDIKKREISEILGALEDPLIDENLSL
EEEEECCHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
NLFDRVKDFSKDCLGLAKRYAQLKASDFKCRVDYSHIPFITIDPKDAKDFDDAIFYDQEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCHHHEECCCC
KVLFVAVADVSEFVPKYSSLDKEARIRGFSVYFPNSVYPMLPLSLSQGACSLKAFEKRLA
CEEEEEEHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
LVYEIPLDDLKNVQLFQGVIEVRANYSYEEINHFLSTQQSSLSKDLQQSLLGFLEMALKL
EEEECCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKERLKKGFNFNSFENKLYLNQEGRIEKIETQQESDAHTLIEEAMLLANQSSARLLDKHF
HHHHHHCCCCCCCCCCEEEECCCCCEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
QNRGIYRTHKEPSLEQQKRLYAKLFDYEITRPKNMGFFPFLEHALKIAKEKNVEREVSRL
HCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
MIKSQNLALYSPMQENHFGLGFASYTHFTSPIRRYSDLALHRLLKELLFHQAKGCSYLLE
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EMPELCSELNALQKKVALIERDFIKRKFARLALEYLEKEFLGVVLETKDEVIVGLKEWVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCC
LKVLVKTNKIFKPLEKVRVKITHAHLILGQVRGEITERIKEHVS
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]