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The map label for this gene is nuoB

Identifier: 109946844

GI number: 109946844

Start: 193532

End: 194011

Strand: Reverse

Name: nuoB

Synonym: Hac_0223

Alternate gene names: 109946844

Gene position: 194011-193532 (Counterclockwise)

Preceding gene: 109946845

Following gene: 109946843

Centisome position: 12.49

GC content: 43.96

Gene sequence:

>480_bases
ATGCAACAAGCACCGGTTGTTCTAAGCACTTTGGATAAATTATTGAATTGGGGGCGTTCTAATTCGCTTTGGCCCTTAAC
CTATGGCTTGGCGTGTTGTGCGATTGAGATGATGGCAACAGGGGGTTCAAGGTTTGATTTTGATAGATTTGGCACGATCT
TTAGAGCGAGTCCTAGACAATCTGATGTGATGATTATCGCTGGCACGCTCACTAAAAAACATGCCGAGTTTATGCGTAGG
CTTTATGATCAAATGCCTGAGCCTAAATGGGTGATTTCTATGGGGAGTTGCGCTAATACGGGTGGGATGTTTAACACTTA
TGCGACCGTTCAAGGAGCGGATAGGATCATTCCTGTGGATATTTATTTACCCGGTTGTGCACCACGCCCAGAGACTTTAC
AATACGCTCTTATGGTTTTGCAAGATAAAATCAGACGCTCTAAAGCGATCAAACAAGATGCCCCAAAAAGGTTAGTGTAA

Upstream 100 bases:

>100_bases
GGCTCGTTGAAATGCTAGGCTTTGTTTTCTTTTTAACGATTGGTTTTATTTACGCTTTAAAGCGAAATGCTTTGAGTTGG
CAAAATTTAGAGGTGAAATA

Downstream 100 bases:

>100_bases
TGGTAAGAAAACAATCCCCCTATGAAGATGTGCAAAAACAATCACGCCAGCATGACCCCTATAAAATCATAGAGCCTACC
CCTAAAAAATATTTAGAGGG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQQAPVVLSTLDKLLNWGRSNSLWPLTYGLACCAIEMMATGGSRFDFDRFGTIFRASPRQSDVMIIAGTLTKKHAEFMRR
LYDQMPEPKWVISMGSCANTGGMFNTYATVQGADRIIPVDIYLPGCAPRPETLQYALMVLQDKIRRSKAIKQDAPKRLV

Sequences:

>Translated_159_residues
MQQAPVVLSTLDKLLNWGRSNSLWPLTYGLACCAIEMMATGGSRFDFDRFGTIFRASPRQSDVMIIAGTLTKKHAEFMRR
LYDQMPEPKWVISMGSCANTGGMFNTYATVQGADRIIPVDIYLPGCAPRPETLQYALMVLQDKIRRSKAIKQDAPKRLV
>Mature_159_residues
MQQAPVVLSTLDKLLNWGRSNSLWPLTYGLACCAIEMMATGGSRFDFDRFGTIFRASPRQSDVMIIAGTLTKKHAEFMRR
LYDQMPEPKWVISMGSCANTGGMFNTYATVQGADRIIPVDIYLPGCAPRPETLQYALMVLQDKIRRSKAIKQDAPKRLV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=59.5890410958904, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=53.4722222222222, Blast_Score=180, Evalue=5e-47,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=39.344262295082, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=35.2459016393443, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=55.7823129251701, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=61.3793103448276, Blast_Score=205, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=61.3793103448276, Blast_Score=205, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24651058, Length=144, Percent_Identity=60.4166666666667, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_HELAH (Q17Z53)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664072.1
- ProteinModelPortal:   Q17Z53
- SMR:   Q17Z53
- STRING:   Q17Z53
- GeneID:   4177453
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0223
- NMPDR:   fig|382638.8.peg.217
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q17Z53
- ProtClustDB:   PRK06411
- BioCyc:   HACI382638:HAC_0223-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17837; Mature: 17837

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQAPVVLSTLDKLLNWGRSNSLWPLTYGLACCAIEMMATGGSRFDFDRFGTIFRASPRQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCC
SDVMIIAGTLTKKHAEFMRRLYDQMPEPKWVISMGSCANTGGMFNTYATVQGADRIIPVD
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEE
IYLPGCAPRPETLQYALMVLQDKIRRSKAIKQDAPKRLV
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQQAPVVLSTLDKLLNWGRSNSLWPLTYGLACCAIEMMATGGSRFDFDRFGTIFRASPRQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCC
SDVMIIAGTLTKKHAEFMRRLYDQMPEPKWVISMGSCANTGGMFNTYATVQGADRIIPVD
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEE
IYLPGCAPRPETLQYALMVLQDKIRRSKAIKQDAPKRLV
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA