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The map label for this gene is nuoM [H]

Identifier: 109946833

GI number: 109946833

Start: 181918

End: 183456

Strand: Reverse

Name: nuoM [H]

Synonym: Hac_0212

Alternate gene names: 109946833

Gene position: 183456-181918 (Counterclockwise)

Preceding gene: 109946834

Following gene: 109946832

Centisome position: 11.81

GC content: 39.57

Gene sequence:

>1539_bases
ATGCAGTATTTACATGCACATCTTTTAAGCGTGGTGATCTTTTTCCCTATGCTAAGTGCCGTATTGGCGTTCTTTATGAG
CGCTCAAGCGAGCAGGGCGTATGCGATTGTCATCGCTTTGATTGAATTGTTATTGGTTTTATTGTTATGGCATGGGTTTG
ATACTCAAGTTACAGGCATGCAGTTTGAAGAAATAAAGGAATTAGTCTATCAAATAGGCGTGAATTACCATGTGGGTATT
GATGGCATCACGCTCTTTTTGTTGCTCTTAAACGCTATTGTGGTGCTTTTATCCGTGATTTATGTCAAAGAGCATCGTAA
GGACTTTGCCATTTGCTTTTTATTGTTAGAAGGGATTTTAATGGGCGTGTTTTCCTCTCTTAACATGATCTTTTTCTACG
CTTTTTGGGAAATCTCGCTCTTGCCGGTTTTATACCTCATCGGTCGTTTTGGACGCAATAACAAAATCTATTCTGGCATG
AAGTTTTTTCTCTACACCTTTTTAGCGTCGTTGTGCATGCTTTTAGGCATTTTATACATTGGGTATTACTACGCTAGCAA
TTACGGCATGATGAGTTTTGATATTTTAGATTGGTATCAATTAAACTTTTCTAGCGAAGTTAAAACTTGGCTCTTTTTGG
CTTTCTTAATAGGGATTGCGGTTAAAATCCCGCTCTTTCCTTTACACACATGGTTGCCTTATGCGTATTCTAACGCCCCT
ACTTTAGGCTCTGTCATGCTTTCAGCCTTGCTTTCTAAAATGGGGACTTACACCTTATTACGCTTCTTGCTCCCGCTTTT
TCCTGAGCTTTCAGAGATCTATTTAACCCCCATAGCTGTTTTAGCGCTATGCATGATCATCTATGGAGGTTTTTTAGCTT
ACACTCAAAAAGATTTAAAAACTCTCATCGCTTATAGCTCGTTTTCGCACATGGGCGTGGTGGTGCTTGGGATTTTTGCA
TTCAATGTTGAGGGGATTTCAGGAGCGGTGTTTATGATGTTTGCGCATGGCATTGTCGCTATGGGGTTATTTTTGCTCGC
CGGTATTTTAGAAGATCGCGCTAGCAGTTTAGAAATCGCTCGCTTTGGATCTATCGCTAAAAACGCTCCTATTTTTGCAG
CCTTTTTTATGATCGTTTTAATGGCGAACATGGGCATGCCTTTAAGCATTGGTTTCGTGGGAGAGTTTTTAAGCTTGTTA
GGGTTTTTTGCCACTTACCCTCTTTTGGCCATCATTGCTGGAACAAGCATTATTTTATCAGCGATTTACATGCTCACTTC
GTATAAAAATGTGTTTTTTGGCAATCTAAAAACTGGGAACAATCAAATCAGCGTGTTTGAAGACTTGAACGCTCGTGAGG
TAGGGGTTTTAAGCGTGATTTTAGCGTTGATTTTAATTTTAGGGATTTACCCCAAAATACTTTTAAAACCGATTGAACAA
GGCTCTAAACAGCTTTTAGAAGTGATAGAAACGCGCTCGCTCCCTTTTTTAGGCTCATTGGACACCAATATAAAAGAGGT
CTCTTATGTTAATAGATAG

Upstream 100 bases:

>100_bases
TAGAGCCAGTCAAGATGGGAATTTAACCTCCATGCTGCGCTTCATGGTGGCTGGGGTTTTAATCTTATTAGCGTTTGTGG
CTGTTTTTTGGAGATAAACA

Downstream 100 bases:

>100_bases
TCTCCATATCTCTTTTGATAGCTTCAATTTTGAGAGCATTTTACCCATGCTTGTGTTGGTGTGTGGGGGGATTTTCACGC
TCTTAATCAACGCTTTCACT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQYLHAHLLSVVIFFPMLSAVLAFFMSAQASRAYAIVIALIELLLVLLLWHGFDTQVTGMQFEEIKELVYQIGVNYHVGI
DGITLFLLLLNAIVVLLSVIYVKEHRKDFAICFLLLEGILMGVFSSLNMIFFYAFWEISLLPVLYLIGRFGRNNKIYSGM
KFFLYTFLASLCMLLGILYIGYYYASNYGMMSFDILDWYQLNFSSEVKTWLFLAFLIGIAVKIPLFPLHTWLPYAYSNAP
TLGSVMLSALLSKMGTYTLLRFLLPLFPELSEIYLTPIAVLALCMIIYGGFLAYTQKDLKTLIAYSSFSHMGVVVLGIFA
FNVEGISGAVFMMFAHGIVAMGLFLLAGILEDRASSLEIARFGSIAKNAPIFAAFFMIVLMANMGMPLSIGFVGEFLSLL
GFFATYPLLAIIAGTSIILSAIYMLTSYKNVFFGNLKTGNNQISVFEDLNAREVGVLSVILALILILGIYPKILLKPIEQ
GSKQLLEVIETRSLPFLGSLDTNIKEVSYVNR

Sequences:

>Translated_512_residues
MQYLHAHLLSVVIFFPMLSAVLAFFMSAQASRAYAIVIALIELLLVLLLWHGFDTQVTGMQFEEIKELVYQIGVNYHVGI
DGITLFLLLLNAIVVLLSVIYVKEHRKDFAICFLLLEGILMGVFSSLNMIFFYAFWEISLLPVLYLIGRFGRNNKIYSGM
KFFLYTFLASLCMLLGILYIGYYYASNYGMMSFDILDWYQLNFSSEVKTWLFLAFLIGIAVKIPLFPLHTWLPYAYSNAP
TLGSVMLSALLSKMGTYTLLRFLLPLFPELSEIYLTPIAVLALCMIIYGGFLAYTQKDLKTLIAYSSFSHMGVVVLGIFA
FNVEGISGAVFMMFAHGIVAMGLFLLAGILEDRASSLEIARFGSIAKNAPIFAAFFMIVLMANMGMPLSIGFVGEFLSLL
GFFATYPLLAIIAGTSIILSAIYMLTSYKNVFFGNLKTGNNQISVFEDLNAREVGVLSVILALILILGIYPKILLKPIEQ
GSKQLLEVIETRSLPFLGSLDTNIKEVSYVNR
>Mature_512_residues
MQYLHAHLLSVVIFFPMLSAVLAFFMSAQASRAYAIVIALIELLLVLLLWHGFDTQVTGMQFEEIKELVYQIGVNYHVGI
DGITLFLLLLNAIVVLLSVIYVKEHRKDFAICFLLLEGILMGVFSSLNMIFFYAFWEISLLPVLYLIGRFGRNNKIYSGM
KFFLYTFLASLCMLLGILYIGYYYASNYGMMSFDILDWYQLNFSSEVKTWLFLAFLIGIAVKIPLFPLHTWLPYAYSNAP
TLGSVMLSALLSKMGTYTLLRFLLPLFPELSEIYLTPIAVLALCMIIYGGFLAYTQKDLKTLIAYSSFSHMGVVVLGIFA
FNVEGISGAVFMMFAHGIVAMGLFLLAGILEDRASSLEIARFGSIAKNAPIFAAFFMIVLMANMGMPLSIGFVGEFLSLL
GFFATYPLLAIIAGTSIILSAIYMLTSYKNVFFGNLKTGNNQISVFEDLNAREVGVLSVILALILILGIYPKILLKPIEQ
GSKQLLEVIETRSLPFLGSLDTNIKEVSYVNR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=372, Percent_Identity=30.9139784946237, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1788613, Length=466, Percent_Identity=34.3347639484979, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1788827, Length=327, Percent_Identity=25.6880733944954, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788831, Length=392, Percent_Identity=25.765306122449, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=391, Percent_Identity=24.8081841432225, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI2367154, Length=414, Percent_Identity=25.1207729468599, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57210; Mature: 57210

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]