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The map label for this gene is rlmN [H]

Identifier: 109946741

GI number: 109946741

Start: 106863

End: 107924

Strand: Direct

Name: rlmN [H]

Synonym: Hac_0116

Alternate gene names: 109946741

Gene position: 106863-107924 (Clockwise)

Preceding gene: 109946740

Following gene: 109946745

Centisome position: 6.88

GC content: 35.59

Gene sequence:

>1062_bases
ATGAAACCTAGCGTTTATGATTTCACTTTAAAGGAATTGAGCCAACTTTTAAAGCCAAGCTTTAGGGCTAAACAACTTTA
TTTGTGGCTTTATGCAAAGTATAAAACGAGCTTTAAAGATATGCAAAATAATTTTTCAAAAGATTTTATCGCTTCTTTGG
AGCAAGAATTTGTTTTGCGCACGATAGAAATCACGCATGTGAGCCATAGCGTTGATGGCTCTAAAAAATACCTTTTTAAA
TCTTTAAAAGATAATCATACTTTTGAAGCGGTGTTTTTGAAAATGAAAGATAAAAAGATTGATGAAGAGACTAACGCCAT
TTTAGAGGGAGAAAAATACACCGTGTGCGTGTCTTGTCAAATAGGCTGTCAAGTGGGTTGCTCGTTTTGTTTCACTCAAA
AAGGCGGTTTTGTAAGGAATTTAAAAGCGAGTGAAATTATCCAACAAGCTTTACTCATTAAAGAGGACAACAACCTCCCC
ATTGAAAAAGCGCTCAATATTGTTTTTATGGGAATGGGCGAGCCTTTGAATAATTTAGATGAGGTGTGTAAAGCGATTGA
GATTTTTAATACCGGCATGCAAATTTCCCCTAAAAGGATCACCATTTCCACTAGCGGCGTAGCGGATAAGATCCCTATTT
TAGCGGAAAAAAACTTGGGCGTGCAATTAGCCATATCCTTACACGCCGTAGATGATAAAACGCGCTCGTCTTTAATACCC
TTGAATAAAAAATATAATATTGAATGCGTTTTGAATGAAGTGAAAAAATGGCCTTTAGAACAACGAAAAAGGGTGATGTT
TGAATACCTTTTGATTAAAAACTTAAACGATAGCCTAAATTGCGCTAAAAAACTTTTAAAACTTTTAAACGGCATTAAAT
CCAAAGTGAATTTGATCTTATTCAATCCGCATGAAGGCTCTAAGTTTGAACGCCCTAGTTTAGAGAGCGCTAGAATGTTT
GCGGATTTTTTAAACTCTAAAGGCTTATTATGCACCATTAGAGAGTCTAAAGCTTTAGATATTGAAGCGGCTTGTGGGCA
ATTGAGAGAGAAAAAACTCTAG

Upstream 100 bases:

>100_bases
TAGAGCGTCATAAGATCCAGCTTTTAGTGTGCGTGGATTCTCACAATAAAGTTTTAGGGGTCTTGCACTTGCACCAACTT
TTAGAATTAGGGCTTAAAGC

Downstream 100 bases:

>100_bases
CAGAAAATTTAAATTTTTTGGTGGCACTTTTCAATATTTATGAATAGTCTTCTATGACAGAGCATGTGAAATAACCCATA
AAAAGCGTGAGCAGCCATAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKPSVYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIASLEQEFVLRTIEITHVSHSVDGSKKYLFK
SLKDNHTFEAVFLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCSFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADKIPILAEKNLGVQLAISLHAVDDKTRSSLIP
LNKKYNIECVLNEVKKWPLEQRKRVMFEYLLIKNLNDSLNCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKL

Sequences:

>Translated_353_residues
MKPSVYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIASLEQEFVLRTIEITHVSHSVDGSKKYLFK
SLKDNHTFEAVFLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCSFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADKIPILAEKNLGVQLAISLHAVDDKTRSSLIP
LNKKYNIECVLNEVKKWPLEQRKRVMFEYLLIKNLNDSLNCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKL
>Mature_353_residues
MKPSVYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIASLEQEFVLRTIEITHVSHSVDGSKKYLFK
SLKDNHTFEAVFLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCSFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADKIPILAEKNLGVQLAISLHAVDDKTRSSLIP
LNKKYNIECVLNEVKKWPLEQRKRVMFEYLLIKNLNDSLNCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=36.6279069767442, Blast_Score=197, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40195; Mature: 40195

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSVYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIASLEQEFVLR
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHE
TIEITHVSHSVDGSKKYLFKSLKDNHTFEAVFLKMKDKKIDEETNAILEGEKYTVCVSCQ
EEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHEECCCEEEEEEEEE
IGCQVGCSFCFTQKGGFVRNLKASEIIQQALLIKEDNNLPIEKALNIVFMGMGEPLNNLD
ECCCCCCEEEEECCCCCEECCCHHHHHHHHHHEECCCCCCHHHCCEEEEEECCCCCHHHH
EVCKAIEIFNTGMQISPKRITISTSGVADKIPILAEKNLGVQLAISLHAVDDKTRSSLIP
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEECCCCHHCCCCC
LNKKYNIECVLNEVKKWPLEQRKRVMFEYLLIKNLNDSLNCAKKLLKLLNGIKSKVNLIL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
FNPHEGSKFERPSLESARMFADFLNSKGLLCTIRESKALDIEAACGQLREKKL
ECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSVYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIASLEQEFVLR
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHE
TIEITHVSHSVDGSKKYLFKSLKDNHTFEAVFLKMKDKKIDEETNAILEGEKYTVCVSCQ
EEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHEECCCEEEEEEEEE
IGCQVGCSFCFTQKGGFVRNLKASEIIQQALLIKEDNNLPIEKALNIVFMGMGEPLNNLD
ECCCCCCEEEEECCCCCEECCCHHHHHHHHHHEECCCCCCHHHCCEEEEEECCCCCHHHH
EVCKAIEIFNTGMQISPKRITISTSGVADKIPILAEKNLGVQLAISLHAVDDKTRSSLIP
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEECCCCHHCCCCC
LNKKYNIECVLNEVKKWPLEQRKRVMFEYLLIKNLNDSLNCAKKLLKLLNGIKSKVNLIL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
FNPHEGSKFERPSLESARMFADFLNSKGLLCTIRESKALDIEAACGQLREKKL
ECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA