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The map label for this gene is pdxJ

Identifier: 109946666

GI number: 109946666

Start: 34902

End: 35690

Strand: Direct

Name: pdxJ

Synonym: Hac_0031

Alternate gene names: 109946666

Gene position: 34902-35690 (Clockwise)

Preceding gene: 109946663

Following gene: 109946667

Centisome position: 2.25

GC content: 37.01

Gene sequence:

>789_bases
ATGCGTTTTGGATTGAATATTGATCATATTGTTACTTTAAGAGAAGTGAGGAAAACTTACGAGCCTGAGATTTTAGAAGC
CCTATTCATCGCTAAAAACACCCATAAAGTGGATTTAATCACTATCCATTTGAGAGAAGACAAACGACACATTCAAAATG
AAGATGTTTTGAGACTGCTTGAGATTAGCCCTTTACCTATTAATATTGAATGCTCTATTAATGCTCATATCACTGATTTT
TTATGCTCCTTAAAGAATAAGCCAAGTAAGGTTACCATCGTGCCTGAAAATAGAAACGAAGTTACGACAGAGGGGGGGTT
GGATTGTTCTCTAAAGGGTTTAGGGGAAACTATTAAGGCGTATCAAAACAAAGGCATTGAAGTGTCTTTATTCATTGATC
CTTTAGAAGACGCTTTGCATTTTGCAAGAGAGCATCAAGTCAAACAAGTGGAGTTTCATACCGGCGTGTATGCGAATTTG
CACAACGCCTTGTATTCTAACGCTAACAATCAAATCCATGCCATTAGTGCGCTCAAAGAAAAAAGCCCTAAAGAGTTGAA
AGAAGAATTACACAACGCCTTTTTGCAATTAAGGAAGATGAGTAAGGAAGCGTTTTTTATGGGTATTATGGTGTGTGCAG
GGCATGGGTTAAATTACTCCAATGTGAAAGAATTATTAAAAATCCCTTCTTTAAGAGAGCTTAATATCGGTCATAGCGTG
ATTTCAAAAGCGGTTTTAGTGGGCTTAGAAAAAGCGATTTTAGAAATGGCGCAACTCATTAAACGCTAA

Upstream 100 bases:

>100_bases
GTTATTATAGCAAAAGATAGCTTGATGATAAAATCTTATACATAGGGAGGGTTTTTGTGTTACAATTTCAAAAATCATTA
TTACAAAATAAAGGATAGCC

Downstream 100 bases:

>100_bases
AATGGTTAAAAAGAAAATTGCGATCAGTTGTGGGGATATTCAAGGTATAGGCTTAGAATTGATTTTAAAAAGCCATAAGG
AAGTGAGCGTGCTTTGTGAG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRFGLNIDHIVTLREVRKTYEPEILEALFIAKNTHKVDLITIHLREDKRHIQNEDVLRLLEISPLPINIECSINAHITDF
LCSLKNKPSKVTIVPENRNEVTTEGGLDCSLKGLGETIKAYQNKGIEVSLFIDPLEDALHFAREHQVKQVEFHTGVYANL
HNALYSNANNQIHAISALKEKSPKELKEELHNAFLQLRKMSKEAFFMGIMVCAGHGLNYSNVKELLKIPSLRELNIGHSV
ISKAVLVGLEKAILEMAQLIKR

Sequences:

>Translated_262_residues
MRFGLNIDHIVTLREVRKTYEPEILEALFIAKNTHKVDLITIHLREDKRHIQNEDVLRLLEISPLPINIECSINAHITDF
LCSLKNKPSKVTIVPENRNEVTTEGGLDCSLKGLGETIKAYQNKGIEVSLFIDPLEDALHFAREHQVKQVEFHTGVYANL
HNALYSNANNQIHAISALKEKSPKELKEELHNAFLQLRKMSKEAFFMGIMVCAGHGLNYSNVKELLKIPSLRELNIGHSV
ISKAVLVGLEKAILEMAQLIKR
>Mature_262_residues
MRFGLNIDHIVTLREVRKTYEPEILEALFIAKNTHKVDLITIHLREDKRHIQNEDVLRLLEISPLPINIECSINAHITDF
LCSLKNKPSKVTIVPENRNEVTTEGGLDCSLKGLGETIKAYQNKGIEVSLFIDPLEDALHFAREHQVKQVEFHTGVYANL
HNALYSNANNQIHAISALKEKSPKELKEELHNAFLQLRKMSKEAFFMGIMVCAGHGLNYSNVKELLKIPSLRELNIGHSV
ISKAVLVGLEKAILEMAQLIKR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=260, Percent_Identity=38.0769230769231, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_HELAH (Q17ZN1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_663894.1
- ProteinModelPortal:   Q17ZN1
- SMR:   Q17ZN1
- STRING:   Q17ZN1
- GeneID:   4176743
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0031
- NMPDR:   fig|382638.8.peg.31
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q17ZN1
- ProtClustDB:   PRK05265
- BioCyc:   HACI382638:HAC_0031-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 29697; Mature: 29697

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 215-215 BINDING 6-6 BINDING 17-17 BINDING 45-45 BINDING 50-50 BINDING 102-102 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFGLNIDHIVTLREVRKTYEPEILEALFIAKNTHKVDLITIHLREDKRHIQNEDVLRLL
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCHHHCCCHHHHHHH
EISPLPINIECSINAHITDFLCSLKNKPSKVTIVPENRNEVTTEGGLDCSLKGLGETIKA
CCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHH
YQNKGIEVSLFIDPLEDALHFAREHQVKQVEFHTGVYANLHNALYSNANNQIHAISALKE
HHCCCCEEEEEECCHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHCCCCCCEEHHHHHHH
KSPKELKEELHNAFLQLRKMSKEAFFMGIMVCAGHGLNYSNVKELLKIPSLRELNIGHSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHH
ISKAVLVGLEKAILEMAQLIKR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFGLNIDHIVTLREVRKTYEPEILEALFIAKNTHKVDLITIHLREDKRHIQNEDVLRLL
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCHHHCCCHHHHHHH
EISPLPINIECSINAHITDFLCSLKNKPSKVTIVPENRNEVTTEGGLDCSLKGLGETIKA
CCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHH
YQNKGIEVSLFIDPLEDALHFAREHQVKQVEFHTGVYANLHNALYSNANNQIHAISALKE
HHCCCCEEEEEECCHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHCCCCCCEEHHHHHHH
KSPKELKEELHNAFLQLRKMSKEAFFMGIMVCAGHGLNYSNVKELLKIPSLRELNIGHSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHH
ISKAVLVGLEKAILEMAQLIKR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA