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The map label for this gene is queF

Identifier: 109946663

GI number: 109946663

Start: 27193

End: 27639

Strand: Direct

Name: queF

Synonym: Hac_0024

Alternate gene names: 109946663

Gene position: 27193-27639 (Clockwise)

Preceding gene: 109946651

Following gene: 109946666

Centisome position: 1.75

GC content: 35.79

Gene sequence:

>447_bases
ATGAGTTCTGAATTAAACCTTAAATTATTAGGCACTAAAACGCCCTATATTTTTGAATACAATAAAGATTTGTTGGAAGC
TTTCCCTAACCCAAACCCCAATTTAGACCCCTTAATCACTTTAGAATGCAAGGAATTTACAAGCCTTTGCCCTATCACTT
CCCAACCGGATTTTGGCGTTGTTTATATCCGCTATATCCCTAAAGATAAAATGGTAGAAAGCAAGTCTTTAAAACTCTAT
TTATTCAGTTATAGAAACCATGGGAGTTTTCATGAAAGTTGTATCAATACGATTTTACTGGATTTAGTGGGATTGTTAGA
GCCAAAGTATTTGGAAGTGTATGGGGATTTTGTCTCTAGGGGAGGGATTGCGATCAAACCCTTTGTGAATTATGCGATCA
AAGAATACCAAGACTTTAAAGAAAAACGCCTTTTGGGTGCGAAATAA

Upstream 100 bases:

>100_bases
TCATCCAATAAAGCCGTAATAATCTCTATGCGTTTGTTCATTGAGTTTCCTAAAAAATGGGTTAAAATAGCCTTATTATA
ACTTAAATCAAGGAATATTC

Downstream 100 bases:

>100_bases
TATGCTGTTAAATATGCATTAACTTTTTGGCTAAAATGAGTTGTTAAAGAATTAAGGAGCGTGAAACAATGAAATGCATT
TTTAAAAATTTGCTTTCTCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSSELNLKLLGTKTPYIFEYNKDLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVVYIRYIPKDKMVESKSLKLY
LFSYRNHGSFHESCINTILLDLVGLLEPKYLEVYGDFVSRGGIAIKPFVNYAIKEYQDFKEKRLLGAK

Sequences:

>Translated_148_residues
MSSELNLKLLGTKTPYIFEYNKDLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVVYIRYIPKDKMVESKSLKLY
LFSYRNHGSFHESCINTILLDLVGLLEPKYLEVYGDFVSRGGIAIKPFVNYAIKEYQDFKEKRLLGAK
>Mature_147_residues
SSELNLKLLGTKTPYIFEYNKDLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGVVYIRYIPKDKMVESKSLKLYL
FSYRNHGSFHESCINTILLDLVGLLEPKYLEVYGDFVSRGGIAIKPFVNYAIKEYQDFKEKRLLGAK

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=89, Percent_Identity=38.2022471910112, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_HELAH (Q17ZN4)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_663891.1
- ProteinModelPortal:   Q17ZN4
- SMR:   Q17ZN4
- STRING:   Q17ZN4
- GeneID:   4176709
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0024
- NMPDR:   fig|382638.8.peg.24
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   CINTILL
- ProtClustDB:   PRK13258
- BioCyc:   HACI382638:HAC_0024-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17026; Mature: 16895

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELNLKLLGTKTPYIFEYNKDLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGV
CCCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCE
VYIRYIPKDKMVESKSLKLYLFSYRNHGSFHESCINTILLDLVGLLEPKYLEVYGDFVSR
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GGIAIKPFVNYAIKEYQDFKEKRLLGAK
CCEEEHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSELNLKLLGTKTPYIFEYNKDLLEAFPNPNPNLDPLITLECKEFTSLCPITSQPDFGV
CCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCE
VYIRYIPKDKMVESKSLKLYLFSYRNHGSFHESCINTILLDLVGLLEPKYLEVYGDFVSR
EEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GGIAIKPFVNYAIKEYQDFKEKRLLGAK
CCEEEHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA