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The map label for this gene is trpA [H]

Identifier: 109946652

GI number: 109946652

Start: 16548

End: 17321

Strand: Reverse

Name: trpA [H]

Synonym: Hac_0013

Alternate gene names: 109946652

Gene position: 17321-16548 (Counterclockwise)

Preceding gene: 109946653

Following gene: 109946645

Centisome position: 1.11

GC content: 37.21

Gene sequence:

>774_bases
ATGTTTGAAACTTTGAAAAAACAAGAAAAAATGGCGTTTATCCCGTTTGTAACCTTGGGCGATCCTGATTATGAACTGAG
TTTTGAAATCATTAAAACTTTAATTGCTAGCGGTGTGAGCGCTTTAGAGTTAGGTTTTGCTTTCTCAGATCCTGTAGCTG
ATGGCATCACTATACAAGCGAGCCATTTAAGGGCGTTAAAACACGCTTCTATGGCTAAAAATTTCCAACTTTTAAAAAAA
ATTAGAGATTATGATTCTCATATTCCCATAGGGCTTTTAGCGTATGCGAATTTAATTTTTTCTTATGGCGTTGATAGTTT
TTACGCTCAAGCTAAAGAATGCGGTACAGATAGCGTTTTAATAGCGGATATGCCCCTGATAGAAAAAGAATTAGTCATCA
AATCCGCTCAAAAACACCAAATTAAACAAATCTTTATCGCTAGCCCTAATGCGAGCGTTAAGGATTTAGAACAAGTAGCC
CTAAATTCGCAAGGCTATATTTACACTTTAGCCAGGAGTGGGGTTACTGGGGCAAGTCATGCTTTAGAAAATGATGCGAG
CGCTGTTATTAAAACCTTAAAAACTTTTAGCCCTATCCCTGCATTACTGGGCTTTGGAATCTCTAAAAAAGAACACATCA
AAAACGCTAAAGGCATGGGGGCTGATGGCGTGATTTGCGGCTCAGCGTTAGTCAAAATCATAGAAGAAAATTTGAATAAT
GAAAACGCCATGCTAGAAAAAATTAAGGGGTTTATAGAAGAAATGGTTTTTTGA

Upstream 100 bases:

>100_bases
AAGAAAGCATTATTGTAGTGAATCTGAGCGGCAGGGGGGATAAAGATTTAAATACCGTTTATAGCGCTTTAAAAGGGGAT
TTAAAATGAGGTATAAAACC

Downstream 100 bases:

>100_bases
AAGGCTTTTTAAAGCTTTTATCGCACCAAAAGGTTAACAATCATCAAAGTTTTTTACCCCATGCGCGTTATGGCGATTGT
TAGTGCAGAAGATACTGCCC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MFETLKKQEKMAFIPFVTLGDPDYELSFEIIKTLIASGVSALELGFAFSDPVADGITIQASHLRALKHASMAKNFQLLKK
IRDYDSHIPIGLLAYANLIFSYGVDSFYAQAKECGTDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVA
LNSQGYIYTLARSGVTGASHALENDASAVIKTLKTFSPIPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIEENLNN
ENAMLEKIKGFIEEMVF

Sequences:

>Translated_257_residues
MFETLKKQEKMAFIPFVTLGDPDYELSFEIIKTLIASGVSALELGFAFSDPVADGITIQASHLRALKHASMAKNFQLLKK
IRDYDSHIPIGLLAYANLIFSYGVDSFYAQAKECGTDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVA
LNSQGYIYTLARSGVTGASHALENDASAVIKTLKTFSPIPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIEENLNN
ENAMLEKIKGFIEEMVF
>Mature_257_residues
MFETLKKQEKMAFIPFVTLGDPDYELSFEIIKTLIASGVSALELGFAFSDPVADGITIQASHLRALKHASMAKNFQLLKK
IRDYDSHIPIGLLAYANLIFSYGVDSFYAQAKECGTDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVA
LNSQGYIYTLARSGVTGASHALENDASAVIKTLKTFSPIPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIEENLNN
ENAMLEKIKGFIEEMVF

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=256, Percent_Identity=46.875, Blast_Score=219, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=30.3418803418803, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28017; Mature: 28017

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETLKKQEKMAFIPFVTLGDPDYELSFEIIKTLIASGVSALELGFAFSDPVADGITIQA
CCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEH
SHLRALKHASMAKNFQLLKKIRDYDSHIPIGLLAYANLIFSYGVDSFYAQAKECGTDSVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEE
IADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVALNSQGYIYTLARSGVTGASH
EECCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHH
ALENDASAVIKTLKTFSPIPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIEENLNN
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
ENAMLEKIKGFIEEMVF
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFETLKKQEKMAFIPFVTLGDPDYELSFEIIKTLIASGVSALELGFAFSDPVADGITIQA
CCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEH
SHLRALKHASMAKNFQLLKKIRDYDSHIPIGLLAYANLIFSYGVDSFYAQAKECGTDSVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEE
IADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVALNSQGYIYTLARSGVTGASH
EECCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHH
ALENDASAVIKTLKTFSPIPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIEENLNN
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
ENAMLEKIKGFIEEMVF
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA