Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 109946653

GI number: 109946653

Start: 17333

End: 18514

Strand: Reverse

Name: trpB [H]

Synonym: Hac_0014

Alternate gene names: 109946653

Gene position: 18514-17333 (Counterclockwise)

Preceding gene: 162139481

Following gene: 109946652

Centisome position: 1.19

GC content: 43.06

Gene sequence:

>1182_bases
ATGAATAAAAAAGCGTATTTTGGGGAGTTTGGGGGGAGTTTTGTTTCAGAATTGTTAGTGCCTGCGTTAAGAGAGTTAGA
ACAAGCGTTTGATGCTTGTTTAAAAGATGATAAATTCCAACAAGAGTATTTCCATCTTTTAAAGGATTTTGTGGGCCGCC
CTAGCCCTTTAACTTTGTGTCAAAATATCATTTCTAACCCTAAAGTTAGGCTTTATCTGAAACGAGAGGATTTAATCCAT
GGCGGAGCGCATAAGACGAACCAAGCGTTAGGGCAAGCCCTTTTGGCTAAAAAAATGGGTAAAACCAGGATCATTGCTGA
AACAGGCGCTGGGCAGCATGGCGTTGCAACGGCTATCGCTTGTGCATTGTTAAATTTAAAATGCGTGATTTTTATGGGAG
GCAAAGACATCAAGCGCCAAGAAATGAACGTTTTCAGAATGCGTTTGTTGGGCGCTGAAATCAGAGAAGTCAATTCAGGG
AGCGCGACACTTAAAGACGCTGTGAATGAGACCTTAAGAGATTGGGCGAGCAGCTACAAAGACACGCACTATTTGCTGGG
CACAGCCGCTGGGCCGCACCCTTACCCTACAATGGTCAAAACCTTTCAAAAAATGATAGGCGATGAGGCCAAACAACAAA
TTCTAGAAAAAGAAAACCGCTTGCCTGATTATGTTATCGCATGCGTTGGAGGGGGGTCTAACGCCATAGGGATATTCAGC
GCGTTTTTAAACGATAAAGAGGTCAAACTCATAGGCGTAGAGCCGGCAGGCTTGGGGCTAGAAACTAATAAGCATGGAGC
AACTTTGAATAAGGGGCGAGTGGGGATTTTGCATGGGAATAAAACCTATCTTTTGCAAGATGATGAAGGCCAAATTATAG
AAAGTCATAGCATAAGCGCTGGGCTTGATTATCCAGGAGTAGGGCCAGAGCACAGCTTTTTAAAAGAAAGCGGGCGTGCG
ATTTATGAAAGTGCAAGCGATATTGAAGCGCTAGAAGCCTTTAGTTTATTATGCCAAAAAGAAGGCATTATCCCAGCCTT
AGAAAGCTCACACGCCTTAGCGTATGCTTTAAAACTCGCTCAAAAATGCACACAAGAAAGCATTATTGTAGTGAATCTGA
GCGGCAGGGGGGATAAAGATTTAAATACCGTTTATAGCGCTTTAAAAGGGGATTTAAAATGA

Upstream 100 bases:

>100_bases
GCGTTGGGTCTGGATTTTAATTCAGGTTTGGAAATAAGCCCTGGTATTAAAAATAAGGATAAAATCAAGCGGTTAGCCCG
AATTTTAAGAGAGTATTAAA

Downstream 100 bases:

>100_bases
GGTATAAAACCATGTTTGAAACTTTGAAAAAACAAGAAAAAATGGCGTTTATCCCGTTTGTAACCTTGGGCGATCCTGAT
TATGAACTGAGTTTTGAAAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDDKFQQEYFHLLKDFVGRPSPLTLCQNIISNPKVRLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLNLKCVIFMGGKDIKRQEMNVFRMRLLGAEIREVNSG
SATLKDAVNETLRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMIGDEAKQQILEKENRLPDYVIACVGGGSNAIGIFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSFLKESGRA
IYESASDIEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCTQESIIVVNLSGRGDKDLNTVYSALKGDLK

Sequences:

>Translated_393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDDKFQQEYFHLLKDFVGRPSPLTLCQNIISNPKVRLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLNLKCVIFMGGKDIKRQEMNVFRMRLLGAEIREVNSG
SATLKDAVNETLRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMIGDEAKQQILEKENRLPDYVIACVGGGSNAIGIFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSFLKESGRA
IYESASDIEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCTQESIIVVNLSGRGDKDLNTVYSALKGDLK
>Mature_393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDDKFQQEYFHLLKDFVGRPSPLTLCQNIISNPKVRLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLNLKCVIFMGGKDIKRQEMNVFRMRLLGAEIREVNSG
SATLKDAVNETLRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMIGDEAKQQILEKENRLPDYVIACVGGGSNAIGIFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSFLKESGRA
IYESASDIEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCTQESIIVVNLSGRGDKDLNTVYSALKGDLK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=65.7142857142857, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=53.125, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42824; Mature: 42824

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDDKFQQEYFHLLKDFVGRPSPLTLC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
QNIISNPKVRLYLKREDLIHGGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIA
HHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
CALLNLKCVIFMGGKDIKRQEMNVFRMRLLGAEIREVNSGSATLKDAVNETLRDWASSYK
HHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
DTHYLLGTAAGPHPYPTMVKTFQKMIGDEAKQQILEKENRLPDYVIACVGGGSNAIGIFS
CCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISA
HHCCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCEEEEEECCCCCEEECCCCCC
GLDYPGVGPEHSFLKESGRAIYESASDIEALEAFSLLCQKEGIIPALESSHALAYALKLA
CCCCCCCCCCHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QKCTQESIIVVNLSGRGDKDLNTVYSALKGDLK
HHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDDKFQQEYFHLLKDFVGRPSPLTLC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
QNIISNPKVRLYLKREDLIHGGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIA
HHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
CALLNLKCVIFMGGKDIKRQEMNVFRMRLLGAEIREVNSGSATLKDAVNETLRDWASSYK
HHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
DTHYLLGTAAGPHPYPTMVKTFQKMIGDEAKQQILEKENRLPDYVIACVGGGSNAIGIFS
CCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISA
HHCCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCEEEEEECCCCCEEECCCCCC
GLDYPGVGPEHSFLKESGRAIYESASDIEALEAFSLLCQKEGIIPALESSHALAYALKLA
CCCCCCCCCCHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QKCTQESIIVVNLSGRGDKDLNTVYSALKGDLK
HHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]