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The map label for this gene is thiDE [H]

Identifier: 110680768

GI number: 110680768

Start: 3474579

End: 3475223

Strand: Direct

Name: thiDE [H]

Synonym: RD1_3611

Alternate gene names: 110680768

Gene position: 3474579-3475223 (Clockwise)

Preceding gene: 110680767

Following gene: 110680769

Centisome position: 84.07

GC content: 63.57

Gene sequence:

>645_bases
ATGACGGGGCTGCTCAAAGACCGTTTGCGGCTTTATCTCGTGACGGACCCGCAGCTTTGCGCCGAGGCCGGTGTTATGAA
CACGGTTCAGCGTGCCGTGGCCGGAGGGGTGACGATGGTGCAGTTGCGCGACAAACACGCAAAGACCCCAGAGCGCATTG
AGCTTGCCATCGCGCTCAAGAATGCCCTGCAGAGCAGCGGCGTGCCACTGGTCATCAACGATGATGTCGTCGCCGCCGTG
GCCACAGATGTGGACGGTGCGCATATCGGTCAGGGCGACATTACCCCCGCTGAGGCACGCGCGATGCTTGGCCCCGGCAG
AATTCTGGGCCTGTCCTGCGAAACCGCGCAAACCGTGCGCGATGCAGACCCCACGCTGGTGGACTACCTCGGGCTGGGGC
CCGTTTTTGGGACGGCAACGAAAGATGACCACGCGCAGCCCATCGGTTTTGACGGGCTGGCGGGTCTGGTCGCGCTGTCG
CCCCTGCCCACTGTCGCGATCGGGGGTCTGAAACACGACCATACCAACGCCGTCGCGGCCAGCGGTGCCGATGGCATGGC
CGTCGTGTCCGCCATCTGTGGTCAGCCCGATCCACAGGCGGCGGCTGGCGCTTTTCATAGTTTCAAACCGGAGCATAAAG
CATGA

Upstream 100 bases:

>100_bases
GCACGGCTGCACAGCGCGCCGACGGGCCGGGCAGTTTCCAGATGCATTTTCTGGACGCGCTTGCCAATACGCAACCTTTT
GAACTGGCGCAGGTCATAAG

Downstream 100 bases:

>100_bases
TACCCAACGTCCTCAGTATCGCCGGGTCTGACCCTTCCGGAGGCGCAGGCATTCAGGCCGACATCAAGGCCATTTCCGCC
AATGGCGCCTTTGCCATGAA

Product: bifunctional ThiED protein

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTGLLKDRLRLYLVTDPQLCAEAGVMNTVQRAVAGGVTMVQLRDKHAKTPERIELAIALKNALQSSGVPLVINDDVVAAV
ATDVDGAHIGQGDITPAEARAMLGPGRILGLSCETAQTVRDADPTLVDYLGLGPVFGTATKDDHAQPIGFDGLAGLVALS
PLPTVAIGGLKHDHTNAVAASGADGMAVVSAICGQPDPQAAAGAFHSFKPEHKA

Sequences:

>Translated_214_residues
MTGLLKDRLRLYLVTDPQLCAEAGVMNTVQRAVAGGVTMVQLRDKHAKTPERIELAIALKNALQSSGVPLVINDDVVAAV
ATDVDGAHIGQGDITPAEARAMLGPGRILGLSCETAQTVRDADPTLVDYLGLGPVFGTATKDDHAQPIGFDGLAGLVALS
PLPTVAIGGLKHDHTNAVAASGADGMAVVSAICGQPDPQAAAGAFHSFKPEHKA
>Mature_213_residues
TGLLKDRLRLYLVTDPQLCAEAGVMNTVQRAVAGGVTMVQLRDKHAKTPERIELAIALKNALQSSGVPLVINDDVVAAVA
TDVDGAHIGQGDITPAEARAMLGPGRILGLSCETAQTVRDADPTLVDYLGLGPVFGTATKDDHAQPIGFDGLAGLVALSP
LPTVAIGGLKHDHTNAVAASGADGMAVVSAICGQPDPQAAAGAFHSFKPEHKA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=178, Percent_Identity=33.7078651685393, Blast_Score=71, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=210, Percent_Identity=36.1904761904762, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21910; Mature: 21779

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLLKDRLRLYLVTDPQLCAEAGVMNTVQRAVAGGVTMVQLRDKHAKTPERIELAIALK
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
NALQSSGVPLVINDDVVAAVATDVDGAHIGQGDITPAEARAMLGPGRILGLSCETAQTVR
HHHHHCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECHHHHHHH
DADPTLVDYLGLGPVFGTATKDDHAQPIGFDGLAGLVALSPLPTVAIGGLKHDHTNAVAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEC
SGADGMAVVSAICGQPDPQAAAGAFHSFKPEHKA
CCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGLLKDRLRLYLVTDPQLCAEAGVMNTVQRAVAGGVTMVQLRDKHAKTPERIELAIALK
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
NALQSSGVPLVINDDVVAAVATDVDGAHIGQGDITPAEARAMLGPGRILGLSCETAQTVR
HHHHHCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECHHHHHHH
DADPTLVDYLGLGPVFGTATKDDHAQPIGFDGLAGLVALSPLPTVAIGGLKHDHTNAVAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEC
SGADGMAVVSAICGQPDPQAAAGAFHSFKPEHKA
CCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA