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The map label for this gene is thiM

Identifier: 110680767

GI number: 110680767

Start: 3473809

End: 3474582

Strand: Direct

Name: thiM

Synonym: RD1_3610

Alternate gene names: 110680767

Gene position: 3473809-3474582 (Clockwise)

Preceding gene: 110680763

Following gene: 110680768

Centisome position: 84.05

GC content: 61.76

Gene sequence:

>774_bases
ATGGCTCCACACGACCTGCTGACGGCTTTGCGCGACCAGAACCCTTTGGTGCACTGCATAACGAATTACGTGGCGATGAA
CATCGCCGCGAACGTGGTCCTGGCAGCGGGCGCATCGCCTGCCATGGTGCACGCCCCCGAAGAAATTGCCGAATTTACGC
CGATTTGCGGTGCGCTGACGATCAACATCGGAACGATTTCCACCCCCTGGCAGGCCAGCATGATGGCGGCGGCAGCAACA
GCGAGCGCGCAAAACATCCCATGGGTTCTGGACCCTGTTGCACATTTCATTTCCGTATATCGAAGAGAAGCGGCCCAGCA
ACTGCTTGCGCAGCGCCCCACGATCCTGCGCGGCAATGCCTCCGAGATACTGGCGCTGACCGGGGAAACGGGGGCCGGTA
AAGGTGCGGACAGCGGGGACAGTGTAGACGCAGCACAAGGCGCGGCGAAAAAGCTGGCGGCACAGTTCGGCACAGTCGTC
GCAATCACCGGACCCGTTGATTATCTGACCGACGGCACGCGGGAAGCGCAGGTTTCCGGCGGCTCGTCCCTGATGCCGCA
GGTCACGGCTTTGGGCTGTTCACAGACGGCGCTGATGGGCGCATATGCGGCCACAGGCCCGGCCTTTGACGCAGCGCTGG
CCGCACTTGCGCATTTCAAGGTAGCAGGCAGCACGGCTGCACAGCGCGCCGACGGGCCGGGCAGTTTCCAGATGCATTTT
CTGGACGCGCTTGCCAATACGCAACCTTTTGAACTGGCGCAGGTCATAAGATGA

Upstream 100 bases:

>100_bases
CCTTTGAACCTGAACCAGATAATGCTGGCGGAGGGAGGTCACATGAGCATTTATGCTACATGGATCACGCCTCTTTTCGC
CCGCGAAACGAGGAAAACAG

Downstream 100 bases:

>100_bases
CGGGGCTGCTCAAAGACCGTTTGCGGCTTTATCTCGTGACGGACCCGCAGCTTTGCGCCGAGGCCGGTGTTATGAACACG
GTTCAGCGTGCCGTGGCCGG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAPHDLLTALRDQNPLVHCITNYVAMNIAANVVLAAGASPAMVHAPEEIAEFTPICGALTINIGTISTPWQASMMAAAAT
ASAQNIPWVLDPVAHFISVYRREAAQQLLAQRPTILRGNASEILALTGETGAGKGADSGDSVDAAQGAAKKLAAQFGTVV
AITGPVDYLTDGTREAQVSGGSSLMPQVTALGCSQTALMGAYAATGPAFDAALAALAHFKVAGSTAAQRADGPGSFQMHF
LDALANTQPFELAQVIR

Sequences:

>Translated_257_residues
MAPHDLLTALRDQNPLVHCITNYVAMNIAANVVLAAGASPAMVHAPEEIAEFTPICGALTINIGTISTPWQASMMAAAAT
ASAQNIPWVLDPVAHFISVYRREAAQQLLAQRPTILRGNASEILALTGETGAGKGADSGDSVDAAQGAAKKLAAQFGTVV
AITGPVDYLTDGTREAQVSGGSSLMPQVTALGCSQTALMGAYAATGPAFDAALAALAHFKVAGSTAAQRADGPGSFQMHF
LDALANTQPFELAQVIR
>Mature_256_residues
APHDLLTALRDQNPLVHCITNYVAMNIAANVVLAAGASPAMVHAPEEIAEFTPICGALTINIGTISTPWQASMMAAAATA
SAQNIPWVLDPVAHFISVYRREAAQQLLAQRPTILRGNASEILALTGETGAGKGADSGDSVDAAQGAAKKLAAQFGTVVA
ITGPVDYLTDGTREAQVSGGSSLMPQVTALGCSQTALMGAYAATGPAFDAALAALAHFKVAGSTAAQRADGPGSFQMHFL
DALANTQPFELAQVIR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=239, Percent_Identity=43.9330543933054, Blast_Score=186, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325042, Length=272, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_ROSDO (Q162K5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683774.1
- ProteinModelPortal:   Q162K5
- SMR:   Q162K5
- STRING:   Q162K5
- GeneID:   4195087
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3610
- NMPDR:   fig|375451.6.peg.3358
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   Q162K5
- BioCyc:   RDEN375451:RD1_3610-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 26310; Mature: 26179

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 117-117 BINDING 163-163 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPHDLLTALRDQNPLVHCITNYVAMNIAANVVLAAGASPAMVHAPEEIAEFTPICGALT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEECHHHHHHHCCCCEEEE
INIGTISTPWQASMMAAAATASAQNIPWVLDPVAHFISVYRREAAQQLLAQRPTILRGNA
EEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
SEILALTGETGAGKGADSGDSVDAAQGAAKKLAAQFGTVVAITGPVDYLTDGTREAQVSG
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCEEECCC
GSSLMPQVTALGCSQTALMGAYAATGPAFDAALAALAHFKVAGSTAAQRADGPGSFQMHF
CCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
LDALANTQPFELAQVIR
HHHHCCCCCHHHHHHCC
>Mature Secondary Structure 
APHDLLTALRDQNPLVHCITNYVAMNIAANVVLAAGASPAMVHAPEEIAEFTPICGALT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEECHHHHHHHCCCCEEEE
INIGTISTPWQASMMAAAATASAQNIPWVLDPVAHFISVYRREAAQQLLAQRPTILRGNA
EEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
SEILALTGETGAGKGADSGDSVDAAQGAAKKLAAQFGTVVAITGPVDYLTDGTREAQVSG
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCEEECCC
GSSLMPQVTALGCSQTALMGAYAATGPAFDAALAALAHFKVAGSTAAQRADGPGSFQMHF
CCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
LDALANTQPFELAQVIR
HHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA