Click here to switch to the map view.

The map label for this gene is glmU

Identifier: 110680484

GI number: 110680484

Start: 3164940

End: 3166292

Strand: Direct

Name: glmU

Synonym: RD1_3304

Alternate gene names: 110680484

Gene position: 3164940-3166292 (Clockwise)

Preceding gene: 110680480

Following gene: 110680485

Centisome position: 76.58

GC content: 60.68

Gene sequence:

>1353_bases
ATGAAAACAGCATTGGTCATCCTTGCGGCGGGCAAAGGCACGCGTATGAATTCCGACCTGCCCAAGGTGCTGCATCCGCT
GGCGGGTGCCCCCCTGCTTATTCATGCGATGCAATCGGGGGCCAGCCTTGGCCCAAGCCGAACGGTCATCGTCGCTGGTC
ACGGCGCGGAACTGGTGCAAAAAGCGGCGCTGTCGCATGACGCCTCCGCCATCATCGTTCAGCAAACCGAACAACTCGGC
ACCGGACATGCCGCGAAACAGGCGCAGGACGCGCTCAAAGGGTTTGACGGTACTGTGGTTGTGCTTTTTGGGGACACGCC
TTTTGTCAGCCCCGATACCCTGTCCGCCATTTGCGACGCCCAACGCTCGGCCGACGTCGTGGTTCTCGGGTTTGAAGCCG
CGGATCCCGCGCGCTATGGGCGACTGGTGATGGATGGCGCGCAGCTTGATCGCATCGTGGAGTATAAAGACGCCACAGAG
GCAGAGCGCGCGATCACCCTGTGCAACAGTGGCGTTGTCGCCTGCAATGGAACGCGTCTGTTTGAATTGCTCGAAGCGGT
GGACAATGACAATGCCGCGGGCGAATTCTACCTGCCCGATATCGTGGGCGTGGCGCGGGCACGGGGGCTGACAGCGGCCG
TTGTGACGTGTGACGAAAGCGAGACGCTGGGGATCAATTCACGCACAGAGCTGTCAGCGGCAGAGGCCGCGTTTCAAGAG
CGCGCCCGCACAAACGCCTTTGAAAACGGCGTCACCCTGCCCGCGCCCGGAACCGTTCATTTCGCCTTCGACACGGTCGT
TGGCCGCGACACGCTTATTGAACCAAACGTCGTTTTTGGCCCCGGTGTGACCATTGAAAGCGGCGCAACCATCCGCGCCT
TCTCGCACCTCGAAGGGTGCCATGTGGCGCGCGGCAGTGTCGTTGGCCCTTACGCGCGCCTGCGCCCCGGCGCGGAACTG
AGCGAGAACGTGCGCGTGGGCAACTTTGTGGAGGTCAAGAACGCCCGCATCGGAACCGGCACGAAAATCAATCACCTCAG
CTACATCGGTGATGCGACCTTGGGCGAATATACAAACGTGGGCGCCGGCACGATCACCTGTAACTATGACGGGGTCTTGA
AACACCATACCGAGATCGGAAACCATGTCTTTATCGGGTCAAACACCATGCTGGTTGCCCCTGTGCAGATCGGTGATCAC
GCCATGACGGGCAGCGGGTCGGTGATCACCTCCGATGTCGAGCCCGAAGCGCTGGCCCTTTCGCGCGCCCCGCAAATCGA
AAAACCCGGCATGGCGACAAAAATCATTAACTTGCTGAGGGCCAAAAAGGCCAAGCAACAGAGAGGCAGCTGA

Upstream 100 bases:

>100_bases
CAGGATTATTGCAACCCGCAACCTTCTTTGATCCACCGTGGCTTGCGCCGCGCGATTTAGCGGATAAACAGACCGGCGAC
AGAACACAAAGAGAATTCGC

Downstream 100 bases:

>100_bases
CCATGTGTGGTATTGTTGGCGTTCTGAGCAAACATGAAGCAGCCCCCATCCTCGTTGACGCGCTCAAGAGGCTGGAATAT
CGCGGCTACGACAGCGCAGG

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKTALVILAAGKGTRMNSDLPKVLHPLAGAPLLIHAMQSGASLGPSRTVIVAGHGAELVQKAALSHDASAIIVQQTEQLG
TGHAAKQAQDALKGFDGTVVVLFGDTPFVSPDTLSAICDAQRSADVVVLGFEAADPARYGRLVMDGAQLDRIVEYKDATE
AERAITLCNSGVVACNGTRLFELLEAVDNDNAAGEFYLPDIVGVARARGLTAAVVTCDESETLGINSRTELSAAEAAFQE
RARTNAFENGVTLPAPGTVHFAFDTVVGRDTLIEPNVVFGPGVTIESGATIRAFSHLEGCHVARGSVVGPYARLRPGAEL
SENVRVGNFVEVKNARIGTGTKINHLSYIGDATLGEYTNVGAGTITCNYDGVLKHHTEIGNHVFIGSNTMLVAPVQIGDH
AMTGSGSVITSDVEPEALALSRAPQIEKPGMATKIINLLRAKKAKQQRGS

Sequences:

>Translated_450_residues
MKTALVILAAGKGTRMNSDLPKVLHPLAGAPLLIHAMQSGASLGPSRTVIVAGHGAELVQKAALSHDASAIIVQQTEQLG
TGHAAKQAQDALKGFDGTVVVLFGDTPFVSPDTLSAICDAQRSADVVVLGFEAADPARYGRLVMDGAQLDRIVEYKDATE
AERAITLCNSGVVACNGTRLFELLEAVDNDNAAGEFYLPDIVGVARARGLTAAVVTCDESETLGINSRTELSAAEAAFQE
RARTNAFENGVTLPAPGTVHFAFDTVVGRDTLIEPNVVFGPGVTIESGATIRAFSHLEGCHVARGSVVGPYARLRPGAEL
SENVRVGNFVEVKNARIGTGTKINHLSYIGDATLGEYTNVGAGTITCNYDGVLKHHTEIGNHVFIGSNTMLVAPVQIGDH
AMTGSGSVITSDVEPEALALSRAPQIEKPGMATKIINLLRAKKAKQQRGS
>Mature_450_residues
MKTALVILAAGKGTRMNSDLPKVLHPLAGAPLLIHAMQSGASLGPSRTVIVAGHGAELVQKAALSHDASAIIVQQTEQLG
TGHAAKQAQDALKGFDGTVVVLFGDTPFVSPDTLSAICDAQRSADVVVLGFEAADPARYGRLVMDGAQLDRIVEYKDATE
AERAITLCNSGVVACNGTRLFELLEAVDNDNAAGEFYLPDIVGVARARGLTAAVVTCDESETLGINSRTELSAAEAAFQE
RARTNAFENGVTLPAPGTVHFAFDTVVGRDTLIEPNVVFGPGVTIESGATIRAFSHLEGCHVARGSVVGPYARLRPGAEL
SENVRVGNFVEVKNARIGTGTKINHLSYIGDATLGEYTNVGAGTITCNYDGVLKHHTEIGNHVFIGSNTMLVAPVQIGDH
AMTGSGSVITSDVEPEALALSRAPQIEKPGMATKIINLLRAKKAKQQRGS

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=446, Percent_Identity=40.5829596412556, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_ROSDO (Q163N8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683491.1
- ProteinModelPortal:   Q163N8
- SMR:   Q163N8
- STRING:   Q163N8
- GeneID:   4195373
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3304
- NMPDR:   fig|375451.6.peg.3071
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   MERTCLA
- PhylomeDB:   Q163N8
- ProtClustDB:   PRK14353
- BioCyc:   RDEN375451:RD1_3304-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR001228
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF01128 IspD; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 47064; Mature: 47064

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 345-345 BINDING 75-75 BINDING 140-140 BINDING 154-154 BINDING 169-169 BINDING 369-369 BINDING 387-387 BINDING 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALVILAAGKGTRMNSDLPKVLHPLAGAPLLIHAMQSGASLGPSRTVIVAGHGAELVQ
CCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEECCCHHHHH
KAALSHDASAIIVQQTEQLGTGHAAKQAQDALKGFDGTVVVLFGDTPFVSPDTLSAICDA
HHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
QRSADVVVLGFEAADPARYGRLVMDGAQLDRIVEYKDATEAERAITLCNSGVVACNGTRL
CCCCCEEEEEECCCCHHHHCEEEECCHHHHHHHHHCCCCHHHHHHEEECCCEEEECCHHH
FELLEAVDNDNAAGEFYLPDIVGVARARGLTAAVVTCDESETLGINSRTELSAAEAAFQE
HHHHHHHCCCCCCCCEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
RARTNAFENGVTLPAPGTVHFAFDTVVGRDTLIEPNVVFGPGVTIESGATIRAFSHLEGC
HHHHHHHHCCEECCCCCEEEEEEEEEECCCCEECCCEEECCCEEEECCCEEEEHHHCCCC
HVARGSVVGPYARLRPGAELSENVRVGNFVEVKNARIGTGTKINHLSYIGDATLGEYTNV
EECCCCEECCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEECEEEECCCCCCCCCCCC
GAGTITCNYDGVLKHHTEIGNHVFIGSNTMLVAPVQIGDHAMTGSGSVITSDVEPEALAL
CCCEEEECCCCHHHHHHHCCCEEEECCCEEEEEEEEECCEEECCCCCEEECCCCCHHHHH
SRAPQIEKPGMATKIINLLRAKKAKQQRGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTALVILAAGKGTRMNSDLPKVLHPLAGAPLLIHAMQSGASLGPSRTVIVAGHGAELVQ
CCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEECCCHHHHH
KAALSHDASAIIVQQTEQLGTGHAAKQAQDALKGFDGTVVVLFGDTPFVSPDTLSAICDA
HHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
QRSADVVVLGFEAADPARYGRLVMDGAQLDRIVEYKDATEAERAITLCNSGVVACNGTRL
CCCCCEEEEEECCCCHHHHCEEEECCHHHHHHHHHCCCCHHHHHHEEECCCEEEECCHHH
FELLEAVDNDNAAGEFYLPDIVGVARARGLTAAVVTCDESETLGINSRTELSAAEAAFQE
HHHHHHHCCCCCCCCEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
RARTNAFENGVTLPAPGTVHFAFDTVVGRDTLIEPNVVFGPGVTIESGATIRAFSHLEGC
HHHHHHHHCCEECCCCCEEEEEEEEEECCCCEECCCEEECCCEEEECCCEEEEHHHCCCC
HVARGSVVGPYARLRPGAELSENVRVGNFVEVKNARIGTGTKINHLSYIGDATLGEYTNV
EECCCCEECCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEECEEEECCCCCCCCCCCC
GAGTITCNYDGVLKHHTEIGNHVFIGSNTMLVAPVQIGDHAMTGSGSVITSDVEPEALAL
CCCEEEECCCCHHHHHHHCCCEEEECCCEEEEEEEEECCEEECCCCCEEECCCCCHHHHH
SRAPQIEKPGMATKIINLLRAKKAKQQRGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA