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The map label for this gene is prfC [H]

Identifier: 110680442

GI number: 110680442

Start: 3126388

End: 3127995

Strand: Direct

Name: prfC [H]

Synonym: RD1_3262

Alternate gene names: 110680442

Gene position: 3126388-3127995 (Clockwise)

Preceding gene: 110680437

Following gene: 110680443

Centisome position: 75.64

GC content: 57.4

Gene sequence:

>1608_bases
ATGCTCGATACACCAAAAAACCGCGCGGATTTACCGCCCGAAATCGCCCGGCGGCGCACCTTTGCGATCATAAGCCACCC
TGATGCCGGCAAGACCACATTGACCGAAAAATTCCTGCTCTTTGGCGGGGCCATCCAGATGGCCGGTCAGGTGCGCGCCA
AAGGCGAAGCACGCCGCACGCGCTCCGACTTCATGGCGATGGAAAAGGACCGCGGCATCTCTGTCTCCGCCTCCGCCATG
TCATTCGACTTCAAGGAGTTCCGGTTCAACCTCGTCGATACGCCCGGCCACAGCGATTTTTCCGAGGATACCTACCGTAC
ACTGACGGCCGTGGATGCAGCGGTGATGGTGATTGACGGGGCGAAAGGCGTGGAGAGCCAGACGCAGAAACTCTTTGAAG
TCTGCCGTATGCGCGACCTGCCCATCTTGACATTTTGCAACAAAATGGACCGCGAAAGCCGGGATGTTTTCGAGATTATT
GACGAAATTCAAGAAAACCTCGCCATTGACGTGACCCCCGCCAGTTGGCCCATCGGGGTGGGGCGTGACTTCATCGGATG
TTACGATATTCTGCGCGACCGCCTTGAATTGATGGACCGCGCCGACCGGAACCGCGTGGCCGAAAGTGTCGAGATCAATG
GCTTGGATGACCCCAAACTGATCGAACTGGTCCCGGGTGAACTGCTTGAAAAACTGCGCGAAGATCTTGAGATGGCGCGC
GAACTTTTGCCGCCCCTCGACATTGCCGCGATGCACGAAGGGTCCCTGACACCGATCTGGTTCGGCTCGGCCATCAATTC
ATTTGGCGTCAAGGAATTGATGGATGGCATCGCCCAATATGGCCCGCAACCACAGATCCAATCCGCCGAGCCACGCCAGA
TTCTGCCCGAGGAAAAGAAAGTATCCGGCTTTGTATTCAAGGTGCAGGCCAACATGGATCCAAAGCACCGCGACCGCGTT
GCATTCGTGCGCATCGCATCAGGCCATTTCAATCGGGGCATGAAACTGACCCACGTGCGTCTGAAAAAGCCGATGGCGAT
TTCCAACCCCGTCATGTTTCTCGCGTCTGACCGCGAGTTGGCCGAAGAGGCATGGGCAGGCGATATCATCGGCATCCCGA
ACCATGGCCAGTTGCGCATCGGCGATACGTTGACCGAAGGCGAAGCGATCCGGGTCACGGGCATCCCCTCCTTTGCGCCG
GAACTTTTGCAAACCGCACGCGCCGGTGATCCACTCAAGGCGAAACATCTGGAAAAAGCGCTGATGCAATTCGCCGAAGA
GGGTGCTGCCAAGGTCTTCAAACCCGCCATCGGATCGGGATTTGTGGTGGGCGTCGTCGGACAGTTGCAATTTGAAGTGT
TGGCCAGCCGCATTGAACTGGAATACGGCCTGCCTGTGCGGTTCGAGGCGTCGCAATTCACCTCGGCGCGCTGGGTCAGT
GGCGACAAACAGGCGGTGGACAAATTCACCGAAAGCAACAAGCAGCATATCGCGACAGATCACGACGGCGATACGGTATA
CCTCACCAGACTGCAGTGGGACATCGACCGGGTGGAGCGGGACCATCCACAGGTGAAGCTAACCGCGACCAAGGAAATGA
TGGTTTAG

Upstream 100 bases:

>100_bases
GCCTTTGCGTAGCATCAAAAACGCATGCTGCACCAGCAGACAAGCGACATATACCGCTGCTCTCTTGCTTTGCCTCATTG
CAGCGCCTATAGCCGCAGGC

Downstream 100 bases:

>100_bases
GCGATTTCGCGCCAATCTGGCGGGCAAACATCTACCCACGCGGGAACTTTCGCAAGATGTGAAGCCAAAGCGCATCGCCA
AGCGCGCAAAGCCGACCTGT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MLDTPKNRADLPPEIARRRTFAIISHPDAGKTTLTEKFLLFGGAIQMAGQVRAKGEARRTRSDFMAMEKDRGISVSASAM
SFDFKEFRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFEVCRMRDLPILTFCNKMDRESRDVFEII
DEIQENLAIDVTPASWPIGVGRDFIGCYDILRDRLELMDRADRNRVAESVEINGLDDPKLIELVPGELLEKLREDLEMAR
ELLPPLDIAAMHEGSLTPIWFGSAINSFGVKELMDGIAQYGPQPQIQSAEPRQILPEEKKVSGFVFKVQANMDPKHRDRV
AFVRIASGHFNRGMKLTHVRLKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDTLTEGEAIRVTGIPSFAP
ELLQTARAGDPLKAKHLEKALMQFAEEGAAKVFKPAIGSGFVVGVVGQLQFEVLASRIELEYGLPVRFEASQFTSARWVS
GDKQAVDKFTESNKQHIATDHDGDTVYLTRLQWDIDRVERDHPQVKLTATKEMMV

Sequences:

>Translated_535_residues
MLDTPKNRADLPPEIARRRTFAIISHPDAGKTTLTEKFLLFGGAIQMAGQVRAKGEARRTRSDFMAMEKDRGISVSASAM
SFDFKEFRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFEVCRMRDLPILTFCNKMDRESRDVFEII
DEIQENLAIDVTPASWPIGVGRDFIGCYDILRDRLELMDRADRNRVAESVEINGLDDPKLIELVPGELLEKLREDLEMAR
ELLPPLDIAAMHEGSLTPIWFGSAINSFGVKELMDGIAQYGPQPQIQSAEPRQILPEEKKVSGFVFKVQANMDPKHRDRV
AFVRIASGHFNRGMKLTHVRLKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDTLTEGEAIRVTGIPSFAP
ELLQTARAGDPLKAKHLEKALMQFAEEGAAKVFKPAIGSGFVVGVVGQLQFEVLASRIELEYGLPVRFEASQFTSARWVS
GDKQAVDKFTESNKQHIATDHDGDTVYLTRLQWDIDRVERDHPQVKLTATKEMMV
>Mature_535_residues
MLDTPKNRADLPPEIARRRTFAIISHPDAGKTTLTEKFLLFGGAIQMAGQVRAKGEARRTRSDFMAMEKDRGISVSASAM
SFDFKEFRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFEVCRMRDLPILTFCNKMDRESRDVFEII
DEIQENLAIDVTPASWPIGVGRDFIGCYDILRDRLELMDRADRNRVAESVEINGLDDPKLIELVPGELLEKLREDLEMAR
ELLPPLDIAAMHEGSLTPIWFGSAINSFGVKELMDGIAQYGPQPQIQSAEPRQILPEEKKVSGFVFKVQANMDPKHRDRV
AFVRIASGHFNRGMKLTHVRLKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDTLTEGEAIRVTGIPSFAP
ELLQTARAGDPLKAKHLEKALMQFAEEGAAKVFKPAIGSGFVVGVVGQLQFEVLASRIELEYGLPVRFEASQFTSARWVS
GDKQAVDKFTESNKQHIATDHDGDTVYLTRLQWDIDRVERDHPQVKLTATKEMMV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=536, Percent_Identity=27.0522388059701, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=26.9709543568465, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=28.8265306122449, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI25306287, Length=511, Percent_Identity=26.614481409002, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI157426893, Length=151, Percent_Identity=31.1258278145695, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=157, Percent_Identity=30.5732484076433, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI4503483, Length=153, Percent_Identity=34.640522875817, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI310110807, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI310123363, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=46.5384615384615, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789738, Length=484, Percent_Identity=26.6528925619835, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788922, Length=174, Percent_Identity=33.9080459770115, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=31.25, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=26.219512195122, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=37.5690607734807, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=30.6666666666667, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=186, Percent_Identity=28.494623655914, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=33.8235294117647, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=33.8235294117647, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=489, Percent_Identity=27.8118609406953, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322359, Length=364, Percent_Identity=27.4725274725275, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=168, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221458488, Length=498, Percent_Identity=28.3132530120482, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=26.9547325102881, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI78706572, Length=187, Percent_Identity=32.0855614973262, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585711, Length=179, Percent_Identity=31.2849162011173, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=179, Percent_Identity=31.2849162011173, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585709, Length=179, Percent_Identity=31.2849162011173, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=32.6388888888889, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59796; Mature: 59796

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTPKNRADLPPEIARRRTFAIISHPDAGKTTLTEKFLLFGGAIQMAGQVRAKGEARRT
CCCCCCCCCCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCHHHCCCHHHHH
RSDFMAMEKDRGISVSASAMSFDFKEFRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDG
HHHHHHHHCCCCCEEEHHHHHCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEC
AKGVESQTQKLFEVCRMRDLPILTFCNKMDRESRDVFEIIDEIQENLAIDVTPASWPIGV
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GRDFIGCYDILRDRLELMDRADRNRVAESVEINGLDDPKLIELVPGELLEKLREDLEMAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHEECCCHHHHHHHHHHHHHHH
ELLPPLDIAAMHEGSLTPIWFGSAINSFGVKELMDGIAQYGPQPQIQSAEPRQILPEEKK
HHCCCCCEEECCCCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
VSGFVFKVQANMDPKHRDRVAFVRIASGHFNRGMKLTHVRLKKPMAISNPVMFLASDREL
CCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEECCHHH
AEEAWAGDIIGIPNHGQLRIGDTLTEGEAIRVTGIPSFAPELLQTARAGDPLKAKHLEKA
HHHHCCCCEEECCCCCCEEECCEECCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
LMQFAEEGAAKVFKPAIGSGFVVGVVGQLQFEVLASRIELEYGLPVRFEASQFTSARWVS
HHHHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHEEECCCEEEEHHHCCCCCCCC
GDKQAVDKFTESNKQHIATDHDGDTVYLTRLQWDIDRVERDHPQVKLTATKEMMV
CCHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECHHHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure
MLDTPKNRADLPPEIARRRTFAIISHPDAGKTTLTEKFLLFGGAIQMAGQVRAKGEARRT
CCCCCCCCCCCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCHHHCCCHHHHH
RSDFMAMEKDRGISVSASAMSFDFKEFRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDG
HHHHHHHHCCCCCEEEHHHHHCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEC
AKGVESQTQKLFEVCRMRDLPILTFCNKMDRESRDVFEIIDEIQENLAIDVTPASWPIGV
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GRDFIGCYDILRDRLELMDRADRNRVAESVEINGLDDPKLIELVPGELLEKLREDLEMAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHEECCCHHHHHHHHHHHHHHH
ELLPPLDIAAMHEGSLTPIWFGSAINSFGVKELMDGIAQYGPQPQIQSAEPRQILPEEKK
HHCCCCCEEECCCCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
VSGFVFKVQANMDPKHRDRVAFVRIASGHFNRGMKLTHVRLKKPMAISNPVMFLASDREL
CCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEECCHHH
AEEAWAGDIIGIPNHGQLRIGDTLTEGEAIRVTGIPSFAPELLQTARAGDPLKAKHLEKA
HHHHCCCCEEECCCCCCEEECCEECCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHH
LMQFAEEGAAKVFKPAIGSGFVVGVVGQLQFEVLASRIELEYGLPVRFEASQFTSARWVS
HHHHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHEEECCCEEEEHHHCCCCCCCC
GDKQAVDKFTESNKQHIATDHDGDTVYLTRLQWDIDRVERDHPQVKLTATKEMMV
CCHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECHHHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA