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The map label for this gene is pyrE

Identifier: 110680417

GI number: 110680417

Start: 3105675

End: 3106352

Strand: Direct

Name: pyrE

Synonym: RD1_3236

Alternate gene names: 110680417

Gene position: 3105675-3106352 (Clockwise)

Preceding gene: 110680416

Following gene: 110680418

Centisome position: 75.14

GC content: 56.78

Gene sequence:

>678_bases
ATGATCCCCTCCGCCTACCCCGATGCGAAAGAAATGGCACGCCTCACGGCCCGCATGCTGCTTGAAATCCACGCGGTACA
TTTCAACGCAAAAGACCCCTTTACGCTCTCCTCCGGTCTGCCCAGCCCGACCTATATTGATTGTCGCAAACTCATCAGCT
TTCCGCGCATCCGCGCCACCTTGATGGATTTCCTGACCGTCACCGTCATGCGCGATGTGGGTTTTGAGGCCTTTGACAAC
ATCGCGGGCGGGGAAACCGCAGGCATTCCCTTTGCTGCATTGGTGGCCGAACGCATGGCCCTGCCCATGACCTATGTGCG
CAAAAAACCCAAAGGCTATGGCCGCAATGCGCGTATCGAAGGCGCGATGGGCGAGGGCGAACGCGTGCTGCTGGTTGAGG
ATCTGACCACCGATGGCGGCTCGAAGCTGTCGTTCGTGGATGCGATCCGCGAGACGGGTGCGACCTGCGGGCATACGGCC
GTGATTTTCTACTATGATATCTTTCCCGAAACCACGAAAACACTGGGCGATCACGGCGTCGCATTGCACTCACTTTGCAC
CTGGTGGGACGTGCTCGCCGAAGCCAAACAGCAAGGCGTTTTCGATGAGGCAACACTCAGCGAAGTGGAGAAATTTCTGC
ACAATCCGCGTAAGTGGCAAGAAGCAAACAAGAAATAA

Upstream 100 bases:

>100_bases
GTGACCGTATTTGACCCCGGATTTGTTCCGAATTGGACGGTGGTGTAATCGTCTGCGCGAAAGCGCCCTGCTCAGCCATC
CAAACACGAAGGACAGCGAC

Downstream 100 bases:

>100_bases
TTTCTTTCCACACCGGGCGCAGTTCAGCACGCGGGGAATCGCGGCACGCACCAAGATGTTGCGCCAAATTTGCACCGTGA
TACTATATGCACGTATACGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MIPSAYPDAKEMARLTARMLLEIHAVHFNAKDPFTLSSGLPSPTYIDCRKLISFPRIRATLMDFLTVTVMRDVGFEAFDN
IAGGETAGIPFAALVAERMALPMTYVRKKPKGYGRNARIEGAMGEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVALHSLCTWWDVLAEAKQQGVFDEATLSEVEKFLHNPRKWQEANKK

Sequences:

>Translated_225_residues
MIPSAYPDAKEMARLTARMLLEIHAVHFNAKDPFTLSSGLPSPTYIDCRKLISFPRIRATLMDFLTVTVMRDVGFEAFDN
IAGGETAGIPFAALVAERMALPMTYVRKKPKGYGRNARIEGAMGEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVALHSLCTWWDVLAEAKQQGVFDEATLSEVEKFLHNPRKWQEANKK
>Mature_225_residues
MIPSAYPDAKEMARLTARMLLEIHAVHFNAKDPFTLSSGLPSPTYIDCRKLISFPRIRATLMDFLTVTVMRDVGFEAFDN
IAGGETAGIPFAALVAERMALPMTYVRKKPKGYGRNARIEGAMGEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTA
VIFYYDIFPETTKTLGDHGVALHSLCTWWDVLAEAKQQGVFDEATLSEVEKFLHNPRKWQEANKK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=195, Percent_Identity=32.8205128205128, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508631, Length=160, Percent_Identity=33.125, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=188, Percent_Identity=29.2553191489362, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17933654, Length=200, Percent_Identity=29.5, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_ROSDO (Q163V5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683424.1
- ProteinModelPortal:   Q163V5
- SMR:   Q163V5
- STRING:   Q163V5
- GeneID:   4198547
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3236
- NMPDR:   fig|375451.6.peg.3006
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   PRK00455
- BioCyc:   RDEN375451:RD1_3236-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 24961; Mature: 24961

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 107-107 BINDING 108-108 BINDING 111-111 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSAYPDAKEMARLTARMLLEIHAVHFNAKDPFTLSSGLPSPTYIDCRKLISFPRIRAT
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCCCCCHHHHHHHHCCHHHHHH
LMDFLTVTVMRDVGFEAFDNIAGGETAGIPFAALVAERMALPMTYVRKKPKGYGRNARIE
HHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEE
GAMGEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTAVIFYYDIFPETTKTLGDHGV
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCH
ALHSLCTWWDVLAEAKQQGVFDEATLSEVEKFLHNPRKWQEANKK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure
MIPSAYPDAKEMARLTARMLLEIHAVHFNAKDPFTLSSGLPSPTYIDCRKLISFPRIRAT
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCCCCCHHHHHHHHCCHHHHHH
LMDFLTVTVMRDVGFEAFDNIAGGETAGIPFAALVAERMALPMTYVRKKPKGYGRNARIE
HHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEE
GAMGEGERVLLVEDLTTDGGSKLSFVDAIRETGATCGHTAVIFYYDIFPETTKTLGDHGV
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCH
ALHSLCTWWDVLAEAKQQGVFDEATLSEVEKFLHNPRKWQEANKK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA