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The map label for this gene is pyrC

Identifier: 110680416

GI number: 110680416

Start: 3104588

End: 3105622

Strand: Direct

Name: pyrC

Synonym: RD1_3235

Alternate gene names: 110680416

Gene position: 3104588-3105622 (Clockwise)

Preceding gene: 110680413

Following gene: 110680417

Centisome position: 75.12

GC content: 61.16

Gene sequence:

>1035_bases
ATGACAAAAACGCTGACCATCCGCCGCCCCGACGACTGGCATCTGCACCTGCGCGATGGCGCGATGCTGCGCGCCGTCCT
GCCCGAAACCACCGCGCATTTCGCCCGCGCGATCATCATGCCAAACCTTGTCCCGCCCGTGGTCACCTTTGCGCAGGCGC
AAGACTATCACGCGCGCATTCTCGCGGCATTGCCGGAAGGTGCAGAGTTTACGCCGCTGATGACGCTTTATCTGACGGAA
GACACCGATCCGGATGATGTGGCCGCCGCGCACGCCAACGGGTTGGTCCATGCGGTCAAGCTTTATCCTGCGGGGGCCAC
GACGAATTCAGCCAGCGGTGTGGCGAATTTTGACAATATCCGCGCCGTGCTGGAGCGTATGGCAGAGATCGGACTGCCGC
TTTGTGTTCATGGCGAGGTTACCGATGATGACATCGACATCTTTGACCGCGAGGCTGTCTTCATCGACCGCGTTCTGGAC
CCGATCCGGCAGGCGACACCGGGGCTGCGGGTGGTCATGGAACACATCACGACGCGCAACGCGGTGGATTATGCCACGGC
ACAGGACGACACGCTCGGCGCGACGATCACGACCCACCATCTGGTGATCAACCGCAACCATATCCTCGCCGGGGGGATCA
AACCTCACTACTATTGCCTGCCCGTGGCCAAACGCGAAGAACACAGGCTGGCGTTGCGCGCTGCGGCAACTTCGGGGGAT
GCGCGGTTTTTCCTCGGCACCGATTCCGCGCCGCATACGGACGCCAACAAGCTGCTGCCCTGTGGCTGCGCCGGGTGCTT
TACCGCGACCAACACCATGTCCATCCTCGCGCATGTTTTCGAGGAAGAACAGGCACTGGAGCGACTGGAAGGATTTGCAT
CCCTGCACGGGCCTGCGTTCTACCGTCTGCCCGCCAACGAAGCACGCCTGACCCTGACAAAAACACCCGCCACCTTCCCG
AGCCATATCGAAACAGAGGATGGGCCAGTGACCGTATTTGACCCCGGATTTGTTCCGAATTGGACGGTGGTGTAA

Upstream 100 bases:

>100_bases
ACCATCGGGCCATCTTGAGACGCCGGGAAAATCAGCCACGCCAAGACGAAACAAACGCCAGCCTCTGGTGCCGCGCGCGT
GCCTATGCTAGCGCAGAAAC

Downstream 100 bases:

>100_bases
TCGTCTGCGCGAAAGCGCCCTGCTCAGCCATCCAAACACGAAGGACAGCGACATGATCCCCTCCGCCTACCCCGATGCGA
AAGAAATGGCACGCCTCACG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTKTLTIRRPDDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARILAALPEGAEFTPLMTLYLTE
DTDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNIRAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLD
PIRQATPGLRVVMEHITTRNAVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGD
ARFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAFYRLPANEARLTLTKTPATFP
SHIETEDGPVTVFDPGFVPNWTVV

Sequences:

>Translated_344_residues
MTKTLTIRRPDDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARILAALPEGAEFTPLMTLYLTE
DTDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNIRAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLD
PIRQATPGLRVVMEHITTRNAVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGD
ARFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAFYRLPANEARLTLTKTPATFP
SHIETEDGPVTVFDPGFVPNWTVV
>Mature_343_residues
TKTLTIRRPDDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARILAALPEGAEFTPLMTLYLTED
TDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNIRAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLDP
IRQATPGLRVVMEHITTRNAVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGDA
RFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAFYRLPANEARLTLTKTPATFPS
HIETEDGPVTVFDPGFVPNWTVV

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=342, Percent_Identity=52.046783625731, Blast_Score=361, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323452, Length=355, Percent_Identity=34.9295774647887, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_ROSDO (Q163V6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683423.1
- ProteinModelPortal:   Q163V6
- SMR:   Q163V6
- STRING:   Q163V6
- GeneID:   4198546
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3235
- NMPDR:   fig|375451.6.peg.3005
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q163V6
- ProtClustDB:   PRK05451
- BioCyc:   RDEN375451:RD1_3235-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37565; Mature: 37434

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLTIRRPDDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARI
CCCEEEEECCCCCEEEEECCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHEEE
LAALPEGAEFTPLMTLYLTEDTDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNI
EEECCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHH
RAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLDPIRQATPGLRVVMEHITTRN
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGD
CCCCCCCCCCCCCEEEEEEEEEEECCEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCC
ARFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAF
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YRLPANEARLTLTKTPATFPSHIETEDGPVTVFDPGFVPNWTVV
EEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEC
>Mature Secondary Structure 
TKTLTIRRPDDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARI
CCEEEEECCCCCEEEEECCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHEEE
LAALPEGAEFTPLMTLYLTEDTDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNI
EEECCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHH
RAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLDPIRQATPGLRVVMEHITTRN
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGD
CCCCCCCCCCCCCEEEEEEEEEEECCEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCC
ARFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAF
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YRLPANEARLTLTKTPATFPSHIETEDGPVTVFDPGFVPNWTVV
EEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA