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The map label for this gene is gltA [H]

Identifier: 110680400

GI number: 110680400

Start: 3085317

End: 3086612

Strand: Direct

Name: gltA [H]

Synonym: RD1_3219

Alternate gene names: 110680400

Gene position: 3085317-3086612 (Clockwise)

Preceding gene: 110680399

Following gene: 110680401

Centisome position: 74.65

GC content: 57.48

Gene sequence:

>1296_bases
ATGGCCGAGAGCACAAAATCAGCAACACTGATCATCGACGGCAAGGAAATCGAACTGCCCATCTACAGCCCGACCGCGGG
GCCCGATGTGATCGACATTGGCAAGCTTTACGCACGGGCGGGCGTGTTCACATATGATCCGGGGTTCACTTCGACCGCAA
GTTGCGACAGCACGATCACCTTCATTGACGGCGGCAAGGGCGAATTGTTGCACCGGGGCTATCCGATTGATCAGCTGGCG
GGCAAATCGCACTATCTCGAAGTCTGCTATTTGCTTCTGTACGGCGAACTGCCGTCTGCCGCGGAACTGGAGAAATTCGA
AGGGCTGATCACGCATCACACGATGCTGCATGAGCAGATGCAGAACTTCTTTCGCGGGTTCCGGCGCGACGCGCATCCAA
TGGCCATCATGGTCGGCGTGGTCGGCGCGATGTCCGCTTTCTATCATGACAGCACCGATATTTCGGACGCGGACCAGCGC
GAGATCGCCAGTCACCGCCTGATCGCCAAAATGCCGACAATCGCCGCATGGGCCTATAAATATTCCATCGGGCAACCCTT
CATCTATCCGCGCAACGATCTGGATTATGCATCGAACTTCCTGCGCATGTGTTTTGCCGTGCCTGCGGAAGACTACGAAG
TGAACCCGATTCTGAGCCGTGCGATGGACCGCATCTTTACGCTGCATGCGGATCATGAGCAGAACGCTTCGACCTCGACC
GTGCGGCTGGCCTCTTCCTCCGGCGCCAACCCCTTTGCCTGTATCGCCGCCGGGATTGCCTGCCTTTGGGGGCCTGCCCA
TGGCGGTGCCAACCAAGCCTGTCTGGAGATGCTGAAAGAGATTGGCACGCCCGACCGCATCCCGGAATTCATCGCGCGCG
CCAAGGACAAGAATGATCCCTATCGCCTGATGGGTTTTGGCCACCGGGTCTATAAAAACCACGACCCGCGCGCCACGGTG
ATGAAGCAAAGCGCGGATGAGGTCCTCGAACTCCTCGGGGTGGATAACAATCCGATCCTGCAGGTCGCAAAAGAGCTCGA
AGCGGCGGCACTGGCTGACCCATATTTTGCGGACAAGAAGCTCTTCCCGAATGTTGATTTCTATTCGGGCATCATTTTGG
AAGCGATGGGCTTTCCGACGTCCATGTTCACGCCGATCTTTGCCGTGGCGCGGACAGTAGGGTGGATTTCTCAGTGGAAA
GAGCAGCTTAGCGACCCTCAGCACAAGATTGGGCGGCCACGGCAGCTTTACCTTGGCGAAGTGAACCGCGATTACTCGGA
CATCGAAACCCGCTGA

Upstream 100 bases:

>100_bases
CACAGGTGCGGTTTCAGCGGTGGATAGAATGGCCTGATCCAAATGCTTCAGGCACCGGGGCGCGACCTGCGACCTGCAAT
GATGAATGGAAGGACCACCT

Downstream 100 bases:

>100_bases
CGCCAGCGATATTGGAAGCATGAGACGGGCTGGGGCGTTTTGCGTTCCGGCCCGTTTTCCATTGTTCTACACCCGAATCG
ACGCCTGCCCCGACCGCTAA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAESTKSATLIIDGKEIELPIYSPTAGPDVIDIGKLYARAGVFTYDPGFTSTASCDSTITFIDGGKGELLHRGYPIDQLA
GKSHYLEVCYLLLYGELPSAAELEKFEGLITHHTMLHEQMQNFFRGFRRDAHPMAIMVGVVGAMSAFYHDSTDISDADQR
EIASHRLIAKMPTIAAWAYKYSIGQPFIYPRNDLDYASNFLRMCFAVPAEDYEVNPILSRAMDRIFTLHADHEQNASTST
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTPDRIPEFIARAKDKNDPYRLMGFGHRVYKNHDPRATV
MKQSADEVLELLGVDNNPILQVAKELEAAALADPYFADKKLFPNVDFYSGIILEAMGFPTSMFTPIFAVARTVGWISQWK
EQLSDPQHKIGRPRQLYLGEVNRDYSDIETR

Sequences:

>Translated_431_residues
MAESTKSATLIIDGKEIELPIYSPTAGPDVIDIGKLYARAGVFTYDPGFTSTASCDSTITFIDGGKGELLHRGYPIDQLA
GKSHYLEVCYLLLYGELPSAAELEKFEGLITHHTMLHEQMQNFFRGFRRDAHPMAIMVGVVGAMSAFYHDSTDISDADQR
EIASHRLIAKMPTIAAWAYKYSIGQPFIYPRNDLDYASNFLRMCFAVPAEDYEVNPILSRAMDRIFTLHADHEQNASTST
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTPDRIPEFIARAKDKNDPYRLMGFGHRVYKNHDPRATV
MKQSADEVLELLGVDNNPILQVAKELEAAALADPYFADKKLFPNVDFYSGIILEAMGFPTSMFTPIFAVARTVGWISQWK
EQLSDPQHKIGRPRQLYLGEVNRDYSDIETR
>Mature_430_residues
AESTKSATLIIDGKEIELPIYSPTAGPDVIDIGKLYARAGVFTYDPGFTSTASCDSTITFIDGGKGELLHRGYPIDQLAG
KSHYLEVCYLLLYGELPSAAELEKFEGLITHHTMLHEQMQNFFRGFRRDAHPMAIMVGVVGAMSAFYHDSTDISDADQRE
IASHRLIAKMPTIAAWAYKYSIGQPFIYPRNDLDYASNFLRMCFAVPAEDYEVNPILSRAMDRIFTLHADHEQNASTSTV
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTPDRIPEFIARAKDKNDPYRLMGFGHRVYKNHDPRATVM
KQSADEVLELLGVDNNPILQVAKELEAAALADPYFADKKLFPNVDFYSGIILEAMGFPTSMFTPIFAVARTVGWISQWKE
QLSDPQHKIGRPRQLYLGEVNRDYSDIETR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=356, Percent_Identity=25.8426966292135, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=65.3488372093023, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=31.8059299191375, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17555174, Length=357, Percent_Identity=26.6106442577031, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=332, Percent_Identity=28.0120481927711, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=334, Percent_Identity=27.8443113772455, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=373, Percent_Identity=25.4691689008043, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640126, Length=356, Percent_Identity=28.0898876404494, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24640124, Length=356, Percent_Identity=28.0898876404494, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356863, Length=368, Percent_Identity=26.9021739130435, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47908; Mature: 47777

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESTKSATLIIDGKEIELPIYSPTAGPDVIDIGKLYARAGVFTYDPGFTSTASCDSTIT
CCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
FIDGGKGELLHRGYPIDQLAGKSHYLEVCYLLLYGELPSAAELEKFEGLITHHTMLHEQM
EEECCCCCCEECCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QNFFRGFRRDAHPMAIMVGVVGAMSAFYHDSTDISDADQREIASHRLIAKMPTIAAWAYK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YSIGQPFIYPRNDLDYASNFLRMCFAVPAEDYEVNPILSRAMDRIFTLHADHEQNASTST
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCE
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTPDRIPEFIARAKDKNDP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
YRLMGFGHRVYKNHDPRATVMKQSADEVLELLGVDNNPILQVAKELEAAALADPYFADKK
EEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LFPNVDFYSGIILEAMGFPTSMFTPIFAVARTVGWISQWKEQLSDPQHKIGRPRQLYLGE
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEC
VNRDYSDIETR
CCCCCHHCCCC
>Mature Secondary Structure 
AESTKSATLIIDGKEIELPIYSPTAGPDVIDIGKLYARAGVFTYDPGFTSTASCDSTIT
CCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
FIDGGKGELLHRGYPIDQLAGKSHYLEVCYLLLYGELPSAAELEKFEGLITHHTMLHEQM
EEECCCCCCEECCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QNFFRGFRRDAHPMAIMVGVVGAMSAFYHDSTDISDADQREIASHRLIAKMPTIAAWAYK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YSIGQPFIYPRNDLDYASNFLRMCFAVPAEDYEVNPILSRAMDRIFTLHADHEQNASTST
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCE
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTPDRIPEFIARAKDKNDP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
YRLMGFGHRVYKNHDPRATVMKQSADEVLELLGVDNNPILQVAKELEAAALADPYFADKK
EEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LFPNVDFYSGIILEAMGFPTSMFTPIFAVARTVGWISQWKEQLSDPQHKIGRPRQLYLGE
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEC
VNRDYSDIETR
CCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]