Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 110680399

GI number: 110680399

Start: 3083810

End: 3085210

Strand: Direct

Name: gltX

Synonym: RD1_3218

Alternate gene names: 110680399

Gene position: 3083810-3085210 (Clockwise)

Preceding gene: 110680397

Following gene: 110680400

Centisome position: 74.61

GC content: 59.74

Gene sequence:

>1401_bases
ATGACTGCGCCCGTTGTTACTCGTTTCGCGCCCTCCCCGACTGGGTTTTTACACATTGGCGGGGCGCGCACGGCTCTGTT
CAACTGGCTCTATGCGCGCGGCAGAGGGGGTAAATTCCTGCTGCGCATCGAGGACACCGACCGCGCGCGCTCAACGCCCG
AGGCGACACAGGCCATTCTGGATGGGATGGCATGGCTCGGGCTGGATCACGACGGCGATATCGTCAGCCAGTTCGACAAC
GCCGCGCGTCATGCCGCCGTGGCCATGGAGCTGCTGGCAGCGGGCAAGGCCTATAAGTGCTTTGCCACGCAAGAAGAGAT
CAGCGCCTTTCGCGAGGCGGCCCGCGCAGACGGGCGTAGTACGCTGTATCGCTCGCCCTGGCGGGATGCGGCACAGGAAG
CGCATCCCGATGCCCCCTTCGTGATCCGCATAAAGGCACCGCAGGAGGGTGAAACCATCATCCGTGATCAGGTGCAGGGG
GATGTTACGATCCGCAACGACCAGCTGGACGACATGGTGCTGTTGCGCTCCGATGGCACGCCCGTCTACATGCTGGCCGT
TGTCGTCGATGATCACGACATGGGCGTGACGCATGTGATCCGGGGCGATGATCACCTTAACAATGCCGCACGCCAGATGA
TGATTTACAACGCCCTCGGCTGGGATGTGCCGGTCTGGGCGCATATCCCGCTGATCCACGGCCCGGATGGCAAGAAACTC
TCCAAACGGCACGGCGCTCTTGGCGCGCAGGAGTATCAGGTCATGGGATACCCTGCGGCGGGCATGCGCAATTATCTGGC
GCGCCTTGGGTGGAGTCATGGGGATGATGAGTTTTTCACCGATGCGCAGGCCAGAGAATGGTTTGATCTGGATGGAATCG
GAAAAAGCCCCGCACGATTTGACACCAAGAAGCTCGAGAACCTCTGCGGGCAGCATATCGCGGCCTCGCAAGATGCTGCA
TTGCGGCAGGAAGCGGAGGCATTCCGCGCTGTTTCAGGCCAGCCGGCGCTGACCGCGTCACAATCACACATGCTTGGAAA
AGCCATGTACTGCATCAAAGAACGCGCGAAGACCTTCCCGGAACTCATTGACAAGGCTCACTTTGCGCTGACACAGCGGC
CAGTCACGCCGGATGCGAAAGCGGCGAAGTCGCTGGATAATGTATCCCGTGGTATACTGAAGGAATTGACGCCGCAGTTG
CAAAATGCTAGCTGGGAGCGAGAAAATCTAGAGGCGATATTGAACGCGTTTGCCCACAGCAAGGACACCAAGTTTGGTAA
GCTGGCCGGCCCTTTGCGCGCTGCCCTGGCCGGGAGATCTGTCACGCCTTCTGTATTTGATATGATGCTGGTTTTGGGTC
CGGAAGAGACCTGCGCACGGCTCAACGATGCTGCTGTTTGA

Upstream 100 bases:

>100_bases
TGATGGCTGCAAGCAGCACCGTCTTGCCCCCGTTCACCGCACTGGATAGACACGGGTTTAGAAATACGCTCCCCATGGTT
ACTGAAAGGTAAACGCAAGC

Downstream 100 bases:

>100_bases
CTGCACCACAGGTGCGGTTTCAGCGGTGGATAGAATGGCCTGATCCAAATGCTTCAGGCACCGGGGCGCGACCTGCGACC
TGCAATGATGAATGGAAGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTAPVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATQAILDGMAWLGLDHDGDIVSQFDN
AARHAAVAMELLAAGKAYKCFATQEEISAFREAARADGRSTLYRSPWRDAAQEAHPDAPFVIRIKAPQEGETIIRDQVQG
DVTIRNDQLDDMVLLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYNALGWDVPVWAHIPLIHGPDGKKL
SKRHGALGAQEYQVMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARFDTKKLENLCGQHIAASQDAA
LRQEAEAFRAVSGQPALTASQSHMLGKAMYCIKERAKTFPELIDKAHFALTQRPVTPDAKAAKSLDNVSRGILKELTPQL
QNASWERENLEAILNAFAHSKDTKFGKLAGPLRAALAGRSVTPSVFDMMLVLGPEETCARLNDAAV

Sequences:

>Translated_466_residues
MTAPVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATQAILDGMAWLGLDHDGDIVSQFDN
AARHAAVAMELLAAGKAYKCFATQEEISAFREAARADGRSTLYRSPWRDAAQEAHPDAPFVIRIKAPQEGETIIRDQVQG
DVTIRNDQLDDMVLLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYNALGWDVPVWAHIPLIHGPDGKKL
SKRHGALGAQEYQVMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARFDTKKLENLCGQHIAASQDAA
LRQEAEAFRAVSGQPALTASQSHMLGKAMYCIKERAKTFPELIDKAHFALTQRPVTPDAKAAKSLDNVSRGILKELTPQL
QNASWERENLEAILNAFAHSKDTKFGKLAGPLRAALAGRSVTPSVFDMMLVLGPEETCARLNDAAV
>Mature_465_residues
TAPVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATQAILDGMAWLGLDHDGDIVSQFDNA
ARHAAVAMELLAAGKAYKCFATQEEISAFREAARADGRSTLYRSPWRDAAQEAHPDAPFVIRIKAPQEGETIIRDQVQGD
VTIRNDQLDDMVLLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYNALGWDVPVWAHIPLIHGPDGKKLS
KRHGALGAQEYQVMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARFDTKKLENLCGQHIAASQDAAL
RQEAEAFRAVSGQPALTASQSHMLGKAMYCIKERAKTFPELIDKAHFALTQRPVTPDAKAAKSLDNVSRGILKELTPQLQ
NASWERENLEAILNAFAHSKDTKFGKLAGPLRAALAGRSVTPSVFDMMLVLGPEETCARLNDAAV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=28.4274193548387, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI4826960, Length=293, Percent_Identity=26.962457337884, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788743, Length=462, Percent_Identity=40.6926406926407, Blast_Score=344, Evalue=7e-96,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=37.1428571428571, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1786895, Length=206, Percent_Identity=27.6699029126214, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=29.6907216494845, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17543332, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=322, Percent_Identity=36.6459627329193, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=184, Percent_Identity=31.5217391304348, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=30.5676855895196, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=31.6430020283976, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_ROSDO (Q163X3)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683406.1
- ProteinModelPortal:   Q163X3
- SMR:   Q163X3
- STRING:   Q163X3
- GeneID:   4198568
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3218
- NMPDR:   fig|375451.6.peg.2988
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   DAQAREW
- PhylomeDB:   Q163X3
- ProtClustDB:   PRK01406
- BioCyc:   RDEN375451:RD1_3218-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51167; Mature: 51035

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATQAIL
CCCCCEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
DGMAWLGLDHDGDIVSQFDNAARHAAVAMELLAAGKAYKCFATQEEISAFREAARADGRS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCH
TLYRSPWRDAAQEAHPDAPFVIRIKAPQEGETIIRDQVQGDVTIRNDQLDDMVLLRSDGT
HHCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCEEEECCCCCCEEEEECCCC
PVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYNALGWDVPVWAHIPLIHGPDGKKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCHH
SKRHGALGAQEYQVMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARF
HHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCC
DTKKLENLCGQHIAASQDAALRQEAEAFRAVSGQPALTASQSHMLGKAMYCIKERAKTFP
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ELIDKAHFALTQRPVTPDAKAAKSLDNVSRGILKELTPQLQNASWERENLEAILNAFAHS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
KDTKFGKLAGPLRAALAGRSVTPSVFDMMLVLGPEETCARLNDAAV
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TAPVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATQAIL
CCCCEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
DGMAWLGLDHDGDIVSQFDNAARHAAVAMELLAAGKAYKCFATQEEISAFREAARADGRS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCH
TLYRSPWRDAAQEAHPDAPFVIRIKAPQEGETIIRDQVQGDVTIRNDQLDDMVLLRSDGT
HHCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCEEEECCCCCCEEEEECCCC
PVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYNALGWDVPVWAHIPLIHGPDGKKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCHH
SKRHGALGAQEYQVMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARF
HHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCC
DTKKLENLCGQHIAASQDAALRQEAEAFRAVSGQPALTASQSHMLGKAMYCIKERAKTFP
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ELIDKAHFALTQRPVTPDAKAAKSLDNVSRGILKELTPQLQNASWERENLEAILNAFAHS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
KDTKFGKLAGPLRAALAGRSVTPSVFDMMLVLGPEETCARLNDAAV
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA