Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 110680397

GI number: 110680397

Start: 3080979

End: 3081680

Strand: Direct

Name: lexA

Synonym: RD1_3216

Alternate gene names: 110680397

Gene position: 3080979-3081680 (Clockwise)

Preceding gene: 110680396

Following gene: 110680399

Centisome position: 74.54

GC content: 60.11

Gene sequence:

>702_bases
ATGCTCACCAAGAAGCAGTTGGATTTGCTGGAATTCATTCATAAACGCGTGCAGCGGGACGGCGTCCCCCCAAGTTTTGA
CGAGATGAAAGAAGCGCTGGATCTGCGGTCCAAATCCGGCATTCACCGGCTGATCACGGCGCTTGAGGAACGCGGCTTTA
TCCGCCGCCTTGCCCACCGCGCCCGTGCGATCGAGATTGTGAAACTGCCCGAAACGCTTGGCGGTGCCGCGCGCGGCGGA
TTTACGCCGCGCGTGATTGAAGGCGACAAACCCGACGCACCATTGCCCGCCGGTGCGCAGGCCGTCAGCAGTGCGGATGC
GGTTGCGCTGCCCCTGGTAGGTCGTATCGCGGCAGGTCTGCCGATCGAGGCGATCAACCAGAATTCTGCCAGCGTGGCGG
TGCCGGGGCAGATGCTGTCTGGCAAGGGCGATCACTATGCACTTGAAGTCAAAGGCGATTCCATGATCGACGCGGGGATC
AACGACGGCGACGTGGTGGTGATCCGCGAAACCTCTGTCGCCGACAATGGCGATATTGTCGTGGCCCTCATCGAAGATCA
CGAAGCGACGCTGAAACGCTATATGCGCAAGGGCAGCTCCATCGCGCTGGAGGCGGCCAACCCGGCCTATGAAACGCGGG
TATTTACCGAAGACAAGGTCAAGGTGCAGGGCAAATTGGTAGGACTGATCCGCACCTATTGA

Upstream 100 bases:

>100_bases
CGGGACTCTGGTCGATTACATTGATCTGTAAAGGTTTTGTTGACACAAAACAGGAACATGAATAGAACAAAAGCACATCA
GGCCTCGGAGGACCAAAAAG

Downstream 100 bases:

>100_bases
CTGCGGTCTTTTGGGGCGCGCCATCTGGTCCAGAGGCGCGTGCCCGTCAAATCCTTGACCGAGACAAGTCGAAGTCCGGC
CTTGTCATGATGAATGGCGA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLTKKQLDLLEFIHKRVQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKLPETLGGAARGG
FTPRVIEGDKPDAPLPAGAQAVSSADAVALPLVGRIAAGLPIEAINQNSASVAVPGQMLSGKGDHYALEVKGDSMIDAGI
NDGDVVVIRETSVADNGDIVVALIEDHEATLKRYMRKGSSIALEAANPAYETRVFTEDKVKVQGKLVGLIRTY

Sequences:

>Translated_233_residues
MLTKKQLDLLEFIHKRVQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKLPETLGGAARGG
FTPRVIEGDKPDAPLPAGAQAVSSADAVALPLVGRIAAGLPIEAINQNSASVAVPGQMLSGKGDHYALEVKGDSMIDAGI
NDGDVVVIRETSVADNGDIVVALIEDHEATLKRYMRKGSSIALEAANPAYETRVFTEDKVKVQGKLVGLIRTY
>Mature_233_residues
MLTKKQLDLLEFIHKRVQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKLPETLGGAARGG
FTPRVIEGDKPDAPLPAGAQAVSSADAVALPLVGRIAAGLPIEAINQNSASVAVPGQMLSGKGDHYALEVKGDSMIDAGI
NDGDVVVIRETSVADNGDIVVALIEDHEATLKRYMRKGSSIALEAANPAYETRVFTEDKVKVQGKLVGLIRTY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=233, Percent_Identity=28.3261802575107, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_ROSDO (Q163X5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683404.1
- ProteinModelPortal:   Q163X5
- SMR:   Q163X5
- STRING:   Q163X5
- MEROPS:   S24.001
- GeneID:   4198566
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3216
- NMPDR:   fig|375451.6.peg.2986
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q163X5
- ProtClustDB:   PRK00215
- BioCyc:   RDEN375451:RD1_3216-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25052; Mature: 25052

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: ACT_SITE 154-154 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKKQLDLLEFIHKRVQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEIVKLPETLGGAARGGFTPRVIEGDKPDAPLPAGAQAVSSADAVALPLVGRIAAGL
HHEEEEEECCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCC
PIEAINQNSASVAVPGQMLSGKGDHYALEVKGDSMIDAGINDGDVVVIRETSVADNGDIV
CHHHCCCCCCEEEECCHHHCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEE
VALIEDHEATLKRYMRKGSSIALEAANPAYETRVFTEDKVKVQGKLVGLIRTY
EEEECCCHHHHHHHHHCCCCEEEEECCCCHHEEEECCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MLTKKQLDLLEFIHKRVQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEIVKLPETLGGAARGGFTPRVIEGDKPDAPLPAGAQAVSSADAVALPLVGRIAAGL
HHEEEEEECCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCC
PIEAINQNSASVAVPGQMLSGKGDHYALEVKGDSMIDAGINDGDVVVIRETSVADNGDIV
CHHHCCCCCCEEEECCHHHCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEE
VALIEDHEATLKRYMRKGSSIALEAANPAYETRVFTEDKVKVQGKLVGLIRTY
EEEECCCHHHHHHHHHCCCCEEEEECCCCHHEEEECCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA