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The map label for this gene is xth [H]

Identifier: 110680357

GI number: 110680357

Start: 3042283

End: 3043065

Strand: Direct

Name: xth [H]

Synonym: RD1_3171

Alternate gene names: 110680357

Gene position: 3042283-3043065 (Clockwise)

Preceding gene: 110680354

Following gene: 110680360

Centisome position: 73.61

GC content: 60.41

Gene sequence:

>783_bases
ATGAAAATTGCAACATTCAACATCAACGGCATCAAGGCACGCATCGACGCCCTGCCCGCCTGGTTGAAAGAAGCCCAGCC
CGACGTGGCCCTCTTGCAGGAGATCAAATCCGTCGACGAGGCCTTTCCACGCACGGTGTTCGAGGACATGGGCTACAATC
TGGAAACCCATGGTCAGAAGAGTTTCAACGGGGTTGCGATCCTGTCCAAACTGCCGCTGGAGGATGTCACACGGGGCCTG
CCCGGCGATGAAACTGATGAACAGGCGCGCTACATTGAGGCGACAGTCATGGGGGATACGGCGCCGGTGCGGGTCTGCGG
TTTGTATCTGCCCAACGGCAACCCGGCACCCGGACCGAAATACGATTACAAACTGGACTGGATGGCGCGGCTATATGACC
GTGCCAAAGCGCTATTGGCCGATGAGACCACCTTTGTGATGGCGGGGGATTACAACATCATCCCGCAAGCCGAAGATGCG
GCCAAACCGGAGAGCTGGCGCGAGGATGCGCTGTTTCGTCTGGAAAGCCGGACGGCGTTTCGCAGGCTGCTCAACCTCGG
GCTGACCGAAGCCTTCCGCGCAAGGACGCAGGGCCCGGGTCACTACAGTTTCTGGGACTATCAGGCGGGGGCGTGGAACC
GCAACAACGGCATCCGCATCGACCATTTGCTGCTGAGCCCGCAAGCGGCGGACATGCTGGTGGATTGCCAGATCGACAAG
GAGGTGCGCGGACGGGACAAGCCTTCGGACCACGTGCCCGTTTGGGTCGATCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GAGAATCACACGCCCGTAGCGACGGGCACAGCGCCACAACGCCCTTGCCCCGGGACGTCACATCAGGGATATCAGAGGCG
TTCAGCACGCGGGGGCCTTC

Downstream 100 bases:

>100_bases
TCAATTGTAAAGACGCGGCATCCCAAGGCTGTCGTGTATTTGATTGACGGTGTCCGGCTTCATGCTCAGACCCTGCGCCA
GGGCATGGAGGTACTGCCCC

Product: exodeoxyribonuclease III, putative

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKIATFNINGIKARIDALPAWLKEAQPDVALLQEIKSVDEAFPRTVFEDMGYNLETHGQKSFNGVAILSKLPLEDVTRGL
PGDETDEQARYIEATVMGDTAPVRVCGLYLPNGNPAPGPKYDYKLDWMARLYDRAKALLADETTFVMAGDYNIIPQAEDA
AKPESWREDALFRLESRTAFRRLLNLGLTEAFRARTQGPGHYSFWDYQAGAWNRNNGIRIDHLLLSPQAADMLVDCQIDK
EVRGRDKPSDHVPVWVDLAA

Sequences:

>Translated_260_residues
MKIATFNINGIKARIDALPAWLKEAQPDVALLQEIKSVDEAFPRTVFEDMGYNLETHGQKSFNGVAILSKLPLEDVTRGL
PGDETDEQARYIEATVMGDTAPVRVCGLYLPNGNPAPGPKYDYKLDWMARLYDRAKALLADETTFVMAGDYNIIPQAEDA
AKPESWREDALFRLESRTAFRRLLNLGLTEAFRARTQGPGHYSFWDYQAGAWNRNNGIRIDHLLLSPQAADMLVDCQIDK
EVRGRDKPSDHVPVWVDLAA
>Mature_260_residues
MKIATFNINGIKARIDALPAWLKEAQPDVALLQEIKSVDEAFPRTVFEDMGYNLETHGQKSFNGVAILSKLPLEDVTRGL
PGDETDEQARYIEATVMGDTAPVRVCGLYLPNGNPAPGPKYDYKLDWMARLYDRAKALLADETTFVMAGDYNIIPQAEDA
AKPESWREDALFRLESRTAFRRLLNLGLTEAFRARTQGPGHYSFWDYQAGAWNRNNGIRIDHLLLSPQAADMLVDCQIDK
EVRGRDKPSDHVPVWVDLAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=28.1954887218045, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=28.1954887218045, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=28.1954887218045, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=37.6425855513308, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=26.9961977186312, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=29.1512915129151, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=29.1512915129151, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29195; Mature: 29195

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNINGIKARIDALPAWLKEAQPDVALLQEIKSVDEAFPRTVFEDMGYNLETHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
SFNGVAILSKLPLEDVTRGLPGDETDEQARYIEATVMGDTAPVRVCGLYLPNGNPAPGPK
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEECCCCCCCCCCC
YDYKLDWMARLYDRAKALLADETTFVMAGDYNIIPQAEDAAKPESWREDALFRLESRTAF
CCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RRLLNLGLTEAFRARTQGPGHYSFWDYQAGAWNRNNGIRIDHLLLSPQAADMLVDCQIDK
HHHHHCCHHHHHHHHCCCCCCCCEECCCCCCEECCCCEEEEEEEECCCHHCEEEEEEECH
EVRGRDKPSDHVPVWVDLAA
HHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIATFNINGIKARIDALPAWLKEAQPDVALLQEIKSVDEAFPRTVFEDMGYNLETHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
SFNGVAILSKLPLEDVTRGLPGDETDEQARYIEATVMGDTAPVRVCGLYLPNGNPAPGPK
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEECCCCCCCCCCC
YDYKLDWMARLYDRAKALLADETTFVMAGDYNIIPQAEDAAKPESWREDALFRLESRTAF
CCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RRLLNLGLTEAFRARTQGPGHYSFWDYQAGAWNRNNGIRIDHLLLSPQAADMLVDCQIDK
HHHHHCCHHHHHHHHCCCCCCCCEECCCCCCEECCCCEEEEEEEECCCHHCEEEEEEECH
EVRGRDKPSDHVPVWVDLAA
HHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]