BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

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The map label for this gene is hadH [H]

Identifier: 110680334

GI number: 110680334

Start: 3022790

End: 3023545

Strand: Direct

Name: hadH [H]

Synonym: RD1_3147

Alternate gene names: 110680334

Gene position: 3022790-3023545 (Clockwise)

Preceding gene: 110680333

Following gene: 110680335

Centisome position: 73.14

GC content: 63.36

Gene sequence:

>756_bases
ATGAAACTCGCTACAACCAATGCGGTGATTACCGGTGCCGCCTCTGGCCTTGGCGAAGCGACGGCACGCCACTTTGCACA
GCACGGCGCACAGGTGACCCTGCTGGACCGCGATGCGGACCGGGGACAGGCCGTCGCGCGTGAGATCGGTATGCATTTTG
TTGAAACGGATGTGACAGATGAGGCTTCGGTCAAGGCCGCCATCGCCACCGCCAAGGAACAGATGAACGGCATCACGGCA
GCGGTGAACTGTGCCGGGATCGTCTATGGAATCAAGACCGTGGGCCGGGATGGCCCCCACCCGCTTGCGCCGTTTTCGCG
CACGCTCGATGTGAACCTTGTCGGGACCTTCAACGTGGCTCGGCTGGCCGCTGAGGCGATGGCCGAAAACGACCCTGAAC
TGGATGGCGCACGCGGTGTCATCATCAACACCGCCTCGATTGCCGCATTTGACGGGCAGAAGGGTCAGGCTGCCTATGCC
GCGTCCAAGGGCGGCGTGGTGGGGATGTGTTTGCCGATGGCGCGCGATCTGGCGCAGGTTGGCGTGCGTGTCATGACAAT
TGCCCCGGGTATCTTCATGACGCCGATGCTGGCCGGGCTCAGCGACGAGATCCAGGCCGAGCTTGCTGCGGATGTGCCAT
GCCCCCGCAGGCTGGGCGAGCCCGCAGAATTCGCGCAACTGGCGGGTTTTATCGTCGAGGCCGGGTATCTGAACGGCGAA
GTCATCCGCATTGATGGCGCGCTGCGGATGCGCTGA

Upstream 100 bases:

>100_bases
ATGATCGACGCCGCCGCGTTACAGTCACCGATCAAGCTGAGTGCTGGCAAAAAGCGTCACGCGATGGTGCAACTGGGCTA
AGGACGAGGAACCCGCAGCA

Downstream 100 bases:

>100_bases
TGCCGCTGCAAAACCGCGTGCAACCCACAGGAGAGATCATCGCTCACCCGGCGCGGGGTCAATTCATGGGCAATCGCGGC
GTTTTACATGACTCAGACCA

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLATTNAVITGAASGLGEATARHFAQHGAQVTLLDRDADRGQAVAREIGMHFVETDVTDEASVKAAIATAKEQMNGITA
AVNCAGIVYGIKTVGRDGPHPLAPFSRTLDVNLVGTFNVARLAAEAMAENDPELDGARGVIINTASIAAFDGQKGQAAYA
ASKGGVVGMCLPMARDLAQVGVRVMTIAPGIFMTPMLAGLSDEIQAELAADVPCPRRLGEPAEFAQLAGFIVEAGYLNGE
VIRIDGALRMR

Sequences:

>Translated_251_residues
MKLATTNAVITGAASGLGEATARHFAQHGAQVTLLDRDADRGQAVAREIGMHFVETDVTDEASVKAAIATAKEQMNGITA
AVNCAGIVYGIKTVGRDGPHPLAPFSRTLDVNLVGTFNVARLAAEAMAENDPELDGARGVIINTASIAAFDGQKGQAAYA
ASKGGVVGMCLPMARDLAQVGVRVMTIAPGIFMTPMLAGLSDEIQAELAADVPCPRRLGEPAEFAQLAGFIVEAGYLNGE
VIRIDGALRMR
>Mature_251_residues
MKLATTNAVITGAASGLGEATARHFAQHGAQVTLLDRDADRGQAVAREIGMHFVETDVTDEASVKAAIATAKEQMNGITA
AVNCAGIVYGIKTVGRDGPHPLAPFSRTLDVNLVGTFNVARLAAEAMAENDPELDGARGVIINTASIAAFDGQKGQAAYA
ASKGGVVGMCLPMARDLAQVGVRVMTIAPGIFMTPMLAGLSDEIQAELAADVPCPRRLGEPAEFAQLAGFIVEAGYLNGE
VIRIDGALRMR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=248, Percent_Identity=55.241935483871, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI83715985, Length=248, Percent_Identity=52.0161290322581, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI40254992, Length=249, Percent_Identity=29.3172690763052, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI15277342, Length=266, Percent_Identity=30.4511278195489, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI59889578, Length=246, Percent_Identity=30.8943089430894, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI31542939, Length=205, Percent_Identity=27.3170731707317, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=28.90625, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87082100, Length=266, Percent_Identity=33.8345864661654, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=30.4, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=30.6201550387597, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=27.4809160305344, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082160, Length=260, Percent_Identity=27.6923076923077, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=30.0395256916996, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789057, Length=263, Percent_Identity=28.1368821292776, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787820, Length=185, Percent_Identity=28.1081081081081, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=24.3027888446215, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=24.7011952191235, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786812, Length=197, Percent_Identity=31.9796954314721, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=245, Percent_Identity=48.9795918367347, Blast_Score=230, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=30.1204819277108, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=29.9212598425197, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72000259, Length=271, Percent_Identity=26.1992619926199, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=27.0588235294118, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=27.0992366412214, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=26.0377358490566, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=26.615969581749, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=271, Percent_Identity=27.3062730627306, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=267, Percent_Identity=26.9662921348315, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=273, Percent_Identity=26.3736263736264, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=260, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=260, Percent_Identity=25.7692307692308, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=27.9850746268657, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=27.2388059701493, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=31.9796954314721, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17737361, Length=247, Percent_Identity=53.8461538461538, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24639444, Length=256, Percent_Identity=35.9375, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=28.957528957529, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355319, Length=262, Percent_Identity=28.2442748091603, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007234, Length=203, Percent_Identity=31.5270935960591, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665243, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221331218, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28571387, Length=212, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24665247, Length=212, Percent_Identity=25.9433962264151, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221331216, Length=212, Percent_Identity=25.9433962264151, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26017; Mature: 26017

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATTNAVITGAASGLGEATARHFAQHGAQVTLLDRDADRGQAVAREIGMHFVETDVTD
CCEECCCEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHEECCCCC
EASVKAAIATAKEQMNGITAAVNCAGIVYGIKTVGRDGPHPLAPFSRTLDVNLVGTFNVA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECHHHH
RLAAEAMAENDPELDGARGVIINTASIAAFDGQKGQAAYAASKGGVVGMCLPMARDLAQV
HHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHH
GVRVMTIAPGIFMTPMLAGLSDEIQAELAADVPCPRRLGEPAEFAQLAGFIVEAGYLNGE
CEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCE
VIRIDGALRMR
EEEECCEEECC
>Mature Secondary Structure
MKLATTNAVITGAASGLGEATARHFAQHGAQVTLLDRDADRGQAVAREIGMHFVETDVTD
CCEECCCEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHEECCCCC
EASVKAAIATAKEQMNGITAAVNCAGIVYGIKTVGRDGPHPLAPFSRTLDVNLVGTFNVA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECHHHH
RLAAEAMAENDPELDGARGVIINTASIAAFDGQKGQAAYAASKGGVVGMCLPMARDLAQV
HHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHH
GVRVMTIAPGIFMTPMLAGLSDEIQAELAADVPCPRRLGEPAEFAQLAGFIVEAGYLNGE
CEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCE
VIRIDGALRMR
EEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]