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The map label for this gene is tyrS

Identifier: 110680333

GI number: 110680333

Start: 3021520

End: 3022770

Strand: Direct

Name: tyrS

Synonym: RD1_3146

Alternate gene names: 110680333

Gene position: 3021520-3022770 (Clockwise)

Preceding gene: 110680330

Following gene: 110680334

Centisome position: 73.11

GC content: 56.35

Gene sequence:

>1251_bases
ATGACCTACATCCCGAAATCCGATTTCATGGCAGTGATGATGGCGCGCGGCTTCATGGCCGATTGCACCGATTATCAAGG
GCTGGATGAAGCCCTGCTGAAGGGTGTTCAACCTGCCTACATCGGTTTTGATGCGACGGCCAAATCCCTGCATGTGGGAT
CGCTGATCCAGATCATGATGCTGCGCTGGTTCCAGAAAACCGGGCACAAACCGCTGACGCTCATGGGGGGTGGGACCACC
AAAGTGGGCGATCCGAGTTTTCGCGCGGACGAACGCCCGCTGTTGGGGCCAGAGGCGATTGACGACAACATCGCCGGAAT
CAAACAGGTGTTCTCCGCCTATATTGATTATTCGGATGCGCCCACCGGGGCCTTGATGCTGAACAATGCCGAATGGCTGG
ACAATCTCAACTACCTCGACTTCCTGCGCGACATCGGGCGGCATTTCTCGATCAACCGGATGCTCAGTTTTGAGAGCGTT
AAATCGCGGCTCGACCGGGAACAATCGCTGTCGTTTCTGGAATTCAACTACATGATCCTGCAGGCCTATGATTTCATGGA
ACTGTATCGCCGCTACGGTTGCATCCTGCAACTTGGCGGATCGGATCAATGGGGCAATATCGTCAACGGTATTGATCTGA
CCCGCCGTGTCATTGATGGCGAGGTCTATGGGCTGACTTCGCCCCTGCTGACGACATCGGACGGCAAGAAGATGGGTAAA
TCCCAATCCGGCGCGGTTTGGCTGAATGCCGATATGTTCTCCCCTTATGAATTCTGGCAGTTCTGGCGCAATACGACGGA
TGCGGATGTCGGGCGGTTTCTGAAACTCTATACCGAGTTGCCCTTGGATGAATGTGAGCGTCTGGGCGCGCTCGCAGGCT
CCGAGATCAATGCCGCCAAGATCACGCTGGCCAACGAAGTCACAACGCTTTTGCACGGCGCAGAGGCGGCGGCGACGGCT
GCACAGACCGCGCGTGACGTCTTTGAAAAAGGCGGGGTTGGGGATGACCTGCCCACCCTGACGCTAAGTGCCGAAGACGT
CGCACAGGGCATTTCCATCGTTCAGCTGATCGTCAAATCGGGTCTGGCCAATTCGGGCAAGGAAGCCAAGCGTCTAATCG
CGGAGAATGGTGCCAAGATGGATGATGCACCCTTGACCAACGCGGGGTTGATGATCGACGCCGCCGCGTTACAGTCACCG
ATCAAGCTGAGTGCTGGCAAAAAGCGTCACGCGATGGTGCAACTGGGCTAA

Upstream 100 bases:

>100_bases
AGTGATCGGTCCCGTTTTTGCGATGGCGCTTTCCAAAGCCTTTTCCTTTGTCTGCACAGCACCTATAGAGTGGGCGCATT
GCAAGCAGAGAGCAAGACCA

Downstream 100 bases:

>100_bases
GGACGAGGAACCCGCAGCAATGAAACTCGCTACAACCAATGCGGTGATTACCGGTGCCGCCTCTGGCCTTGGCGAAGCGA
CGGCACGCCACTTTGCACAG

Product: tyrosyl-tRNA synthetase, putative

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTYIPKSDFMAVMMARGFMADCTDYQGLDEALLKGVQPAYIGFDATAKSLHVGSLIQIMMLRWFQKTGHKPLTLMGGGTT
KVGDPSFRADERPLLGPEAIDDNIAGIKQVFSAYIDYSDAPTGALMLNNAEWLDNLNYLDFLRDIGRHFSINRMLSFESV
KSRLDREQSLSFLEFNYMILQAYDFMELYRRYGCILQLGGSDQWGNIVNGIDLTRRVIDGEVYGLTSPLLTTSDGKKMGK
SQSGAVWLNADMFSPYEFWQFWRNTTDADVGRFLKLYTELPLDECERLGALAGSEINAAKITLANEVTTLLHGAEAAATA
AQTARDVFEKGGVGDDLPTLTLSAEDVAQGISIVQLIVKSGLANSGKEAKRLIAENGAKMDDAPLTNAGLMIDAAALQSP
IKLSAGKKRHAMVQLG

Sequences:

>Translated_416_residues
MTYIPKSDFMAVMMARGFMADCTDYQGLDEALLKGVQPAYIGFDATAKSLHVGSLIQIMMLRWFQKTGHKPLTLMGGGTT
KVGDPSFRADERPLLGPEAIDDNIAGIKQVFSAYIDYSDAPTGALMLNNAEWLDNLNYLDFLRDIGRHFSINRMLSFESV
KSRLDREQSLSFLEFNYMILQAYDFMELYRRYGCILQLGGSDQWGNIVNGIDLTRRVIDGEVYGLTSPLLTTSDGKKMGK
SQSGAVWLNADMFSPYEFWQFWRNTTDADVGRFLKLYTELPLDECERLGALAGSEINAAKITLANEVTTLLHGAEAAATA
AQTARDVFEKGGVGDDLPTLTLSAEDVAQGISIVQLIVKSGLANSGKEAKRLIAENGAKMDDAPLTNAGLMIDAAALQSP
IKLSAGKKRHAMVQLG
>Mature_415_residues
TYIPKSDFMAVMMARGFMADCTDYQGLDEALLKGVQPAYIGFDATAKSLHVGSLIQIMMLRWFQKTGHKPLTLMGGGTTK
VGDPSFRADERPLLGPEAIDDNIAGIKQVFSAYIDYSDAPTGALMLNNAEWLDNLNYLDFLRDIGRHFSINRMLSFESVK
SRLDREQSLSFLEFNYMILQAYDFMELYRRYGCILQLGGSDQWGNIVNGIDLTRRVIDGEVYGLTSPLLTTSDGKKMGKS
QSGAVWLNADMFSPYEFWQFWRNTTDADVGRFLKLYTELPLDECERLGALAGSEINAAKITLANEVTTLLHGAEAAATAA
QTARDVFEKGGVGDDLPTLTLSAEDVAQGISIVQLIVKSGLANSGKEAKRLIAENGAKMDDAPLTNAGLMIDAAALQSPI
KLSAGKKRHAMVQLG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=406, Percent_Identity=34.9753694581281, Blast_Score=218, Evalue=8e-57,
Organism=Escherichia coli, GI1787925, Length=388, Percent_Identity=42.0103092783505, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25145802, Length=317, Percent_Identity=32.807570977918, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25145805, Length=241, Percent_Identity=31.9502074688797, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=308, Percent_Identity=38.6363636363636, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20130383, Length=351, Percent_Identity=40.7407407407407, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_ROSDO (Q164D9)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683340.1
- ProteinModelPortal:   Q164D9
- SMR:   Q164D9
- STRING:   Q164D9
- GeneID:   4198652
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3146
- NMPDR:   fig|375451.6.peg.2921
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q164D9
- ProtClustDB:   PRK05912
- BioCyc:   RDEN375451:RD1_3146-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45591; Mature: 45459

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 40-40 BINDING 177-177 BINDING 181-181 BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYIPKSDFMAVMMARGFMADCTDYQGLDEALLKGVQPAYIGFDATAKSLHVGSLIQIMM
CCCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHH
LRWFQKTGHKPLTLMGGGTTKVGDPSFRADERPLLGPEAIDDNIAGIKQVFSAYIDYSDA
HHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCC
PTGALMLNNAEWLDNLNYLDFLRDIGRHFSINRMLSFESVKSRLDREQSLSFLEFNYMIL
CCCEEEECCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHH
QAYDFMELYRRYGCILQLGGSDQWGNIVNGIDLTRRVIDGEVYGLTSPLLTTSDGKKMGK
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHEECCCCCEECCCCHHHCC
SQSGAVWLNADMFSPYEFWQFWRNTTDADVGRFLKLYTELPLDECERLGALAGSEINAAK
CCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
ITLANEVTTLLHGAEAAATAAQTARDVFEKGGVGDDLPTLTLSAEDVAQGISIVQLIVKS
EEHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
GLANSGKEAKRLIAENGAKMDDAPLTNAGLMIDAAALQSPIKLSAGKKRHAMVQLG
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
TYIPKSDFMAVMMARGFMADCTDYQGLDEALLKGVQPAYIGFDATAKSLHVGSLIQIMM
CCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHH
LRWFQKTGHKPLTLMGGGTTKVGDPSFRADERPLLGPEAIDDNIAGIKQVFSAYIDYSDA
HHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCC
PTGALMLNNAEWLDNLNYLDFLRDIGRHFSINRMLSFESVKSRLDREQSLSFLEFNYMIL
CCCEEEECCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHH
QAYDFMELYRRYGCILQLGGSDQWGNIVNGIDLTRRVIDGEVYGLTSPLLTTSDGKKMGK
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHEECCCCCEECCCCHHHCC
SQSGAVWLNADMFSPYEFWQFWRNTTDADVGRFLKLYTELPLDECERLGALAGSEINAAK
CCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
ITLANEVTTLLHGAEAAATAAQTARDVFEKGGVGDDLPTLTLSAEDVAQGISIVQLIVKS
EEHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
GLANSGKEAKRLIAENGAKMDDAPLTNAGLMIDAAALQSPIKLSAGKKRHAMVQLG
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA