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The map label for this gene is eno [H]

Identifier: 110680329

GI number: 110680329

Start: 3017362

End: 3018636

Strand: Direct

Name: eno [H]

Synonym: RD1_3142

Alternate gene names: 110680329

Gene position: 3017362-3018636 (Clockwise)

Preceding gene: 110680328

Following gene: 110680330

Centisome position: 73.0

GC content: 59.14

Gene sequence:

>1275_bases
ATGAGCACGATCATCGACATTCACGCCCGCGAAATTCTGGACAGCCGTGGCAATCCAACGGTCGAAGTTGATGTGATCCT
CGAAGATGGCACGATGGGGCGCGCCGCCGTGCCCTCCGGTGCCTCGACAGGCGCCTACGAGGCGGTGGAACGCCGCGACG
GGGACAAGACCCGCTATATGGGCAAGGGCGTGCTGGAGGCGTGCGCCGCTGTCAACGGTGAGATCGCGGATGCGCTGGTG
GGTCTGGACGCCACTGAGCAGGTCGACATTGATGCAAGCATGATCGAACTGGACGGCACGGCGAACAAATCCCGCCTTGG
CGCGAACGCCATTCTGGGCGTGTCACTGGCCGCCGCAAAGGCTGCTGCGGATTACTGCACGCAGCCCCTCTACCGCTATG
TCGGGGGTACATCGGCGCGTGTCCTGCCCGTTCCCATGATGAACATCATCAACGGTGGCGAGCATGCGGACAACCCGATC
GACATTCAGGAATTCATGATCATGCCGGTGGCCGCCGACAACATCCGTGACGCCGTGCGCATGGGATCAGAAGTCTTCCA
TACACTGAAAAAAGAGCTCTCCGCCGCGGGCCTCTCCACGGGCATCGGTGATGAAGGTGGCTTTGCACCCAACATCAACT
CCACCCGCGACGCACTTGATTTCATTTTGAAATCCATCGAAAAGGCAGGGTACAAACCGGGGGAGGAGATTTACCTCGCT
CTGGATTGCGCGGCGACAGAATATTTCAAGAATGGCAAATACGAGTTGTCAGGTGAAGGCAAATCGCTGACGTCTGAGGA
AAACGTGGCCTATCTGGCGGCATTGGTCGCGGATTACCCGATCATCTCCATCGAGGATGCGATGTCCGAAGATGACTGGG
ACGGCTGGAAGGCGCTGACGGACGCCTTGGGCCACAAGGTTCAACTGGTCGGGGATGATCTGTTCGTCACCAACCCTGAG
CGTCTCGCCACAGGCATCGAACGTGGATCGGCCAACTCGATGCTGGTCAAGGTCAACCAGATCGGCACCCTGACCGAAAC
GCTCAAGGCCGTGGATATGGCCCATCGCGCCGGCTTCACCAATGTGATGTCGCACCGCTCCGGAGAGACCGAAGACGCCA
CAATCGCGGATCTTGCGGTCGCCACGAACTGCGGTCAGATCAAGACCGGCTCGCTTGCCCGCTCTGACAGGCTGGCAAAA
TACAACCAGTTGATCCGGATCGAGGAAGCCTTGGGCGAAACAGCGGAATATGCCGGTCGTTCAATCTTGCGATGA

Upstream 100 bases:

>100_bases
TGGCGCGAACGCCGGGTCCGGGGCGGGGCGCACTAGGCCTTTCCATCCTTGAGCCGTCGGGCTATCACCTCGGCAAATTC
ATATGCAGGAGCCCACACTC

Downstream 100 bases:

>100_bases
GCCCGGTGATCACGATCAGGCCCGCGCGAACGTCCGATGAACTTGACGATGTTCGGCGGCTCTGCTGGGAGTATCGCGAC
TATCTGCTGGGTTTTGACGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSTIIDIHAREILDSRGNPTVEVDVILEDGTMGRAAVPSGASTGAYEAVERRDGDKTRYMGKGVLEACAAVNGEIADALV
GLDATEQVDIDASMIELDGTANKSRLGANAILGVSLAAAKAAADYCTQPLYRYVGGTSARVLPVPMMNIINGGEHADNPI
DIQEFMIMPVAADNIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNINSTRDALDFILKSIEKAGYKPGEEIYLA
LDCAATEYFKNGKYELSGEGKSLTSEENVAYLAALVADYPIISIEDAMSEDDWDGWKALTDALGHKVQLVGDDLFVTNPE
RLATGIERGSANSMLVKVNQIGTLTETLKAVDMAHRAGFTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEALGETAEYAGRSILR

Sequences:

>Translated_424_residues
MSTIIDIHAREILDSRGNPTVEVDVILEDGTMGRAAVPSGASTGAYEAVERRDGDKTRYMGKGVLEACAAVNGEIADALV
GLDATEQVDIDASMIELDGTANKSRLGANAILGVSLAAAKAAADYCTQPLYRYVGGTSARVLPVPMMNIINGGEHADNPI
DIQEFMIMPVAADNIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNINSTRDALDFILKSIEKAGYKPGEEIYLA
LDCAATEYFKNGKYELSGEGKSLTSEENVAYLAALVADYPIISIEDAMSEDDWDGWKALTDALGHKVQLVGDDLFVTNPE
RLATGIERGSANSMLVKVNQIGTLTETLKAVDMAHRAGFTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEALGETAEYAGRSILR
>Mature_423_residues
STIIDIHAREILDSRGNPTVEVDVILEDGTMGRAAVPSGASTGAYEAVERRDGDKTRYMGKGVLEACAAVNGEIADALVG
LDATEQVDIDASMIELDGTANKSRLGANAILGVSLAAAKAAADYCTQPLYRYVGGTSARVLPVPMMNIINGGEHADNPID
IQEFMIMPVAADNIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNINSTRDALDFILKSIEKAGYKPGEEIYLAL
DCAATEYFKNGKYELSGEGKSLTSEENVAYLAALVADYPIISIEDAMSEDDWDGWKALTDALGHKVQLVGDDLFVTNPER
LATGIERGSANSMLVKVNQIGTLTETLKAVDMAHRAGFTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEALGETAEYAGRSILR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=51.8518518518518, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=52.0833333333333, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=50.4672897196262, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=50.4672897196262, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=46.0093896713615, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=24.7058823529412, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=24.7058823529412, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=24.7058823529412, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=62.7039627039627, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.4722222222222, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.4722222222222, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=49.2227979274611, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=51.2761020881671, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=50.3448275862069, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=50.1149425287356, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=50.1149425287356, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=50.8120649651972, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=49.8850574712644, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=49.8850574712644, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=49.8850574712644, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=49.8850574712644, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=49.8850574712644, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=49.8850574712644, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45147; Mature: 45016

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIDIHAREILDSRGNPTVEVDVILEDGTMGRAAVPSGASTGAYEAVERRDGDKTRYM
CCCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEACAAVNGEIADALVGLDATEQVDIDASMIELDGTANKSRLGANAILGVSLAAAK
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHH
AAADYCTQPLYRYVGGTSARVLPVPMMNIINGGEHADNPIDIQEFMIMPVAADNIRDAVR
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEEECCHHHHHHHHH
MGSEVFHTLKKELSAAGLSTGIGDEGGFAPNINSTRDALDFILKSIEKAGYKPGEEIYLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAATEYFKNGKYELSGEGKSLTSEENVAYLAALVADYPIISIEDAMSEDDWDGWKALT
EEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHH
DALGHKVQLVGDDLFVTNPERLATGIERGSANSMLVKVNQIGTLTETLKAVDMAHRAGFT
HHHCCEEEEECCCEEEECHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHH
NVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEALGETAEYAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SILR
HHCC
>Mature Secondary Structure 
STIIDIHAREILDSRGNPTVEVDVILEDGTMGRAAVPSGASTGAYEAVERRDGDKTRYM
CCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEACAAVNGEIADALVGLDATEQVDIDASMIELDGTANKSRLGANAILGVSLAAAK
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHH
AAADYCTQPLYRYVGGTSARVLPVPMMNIINGGEHADNPIDIQEFMIMPVAADNIRDAVR
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEEECCHHHHHHHHH
MGSEVFHTLKKELSAAGLSTGIGDEGGFAPNINSTRDALDFILKSIEKAGYKPGEEIYLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAATEYFKNGKYELSGEGKSLTSEENVAYLAALVADYPIISIEDAMSEDDWDGWKALT
EEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHH
DALGHKVQLVGDDLFVTNPERLATGIERGSANSMLVKVNQIGTLTETLKAVDMAHRAGFT
HHHCCEEEEECCCEEEECHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHH
NVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEALGETAEYAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SILR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA