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The map label for this gene is queA

Identifier: 110680196

GI number: 110680196

Start: 2875541

End: 2876596

Strand: Reverse

Name: queA

Synonym: RD1_2998

Alternate gene names: 110680196

Gene position: 2876596-2875541 (Counterclockwise)

Preceding gene: 110680197

Following gene: 110680195

Centisome position: 69.6

GC content: 61.17

Gene sequence:

>1056_bases
ATGAAGCTCAGTGATTTTGATTTCGACCTGCCCGAAGACCTGATTGCGACCCGCCCCGCCGTGCCGCGCACCTCTGCGCG
CCTGCTTGTGGCGAAAGGGCGGCAGATCAGCGATTCGATCGTTTCCGACCTGCCAAACTGGCTGTCTCCGGGCGACCGGC
TGGTGTTGAATGACACGCGGGTGATCCCGGCGCGACTGACCGGTGCGCGGCACCGCGACACCGCACAGGGACCGATGCAG
GCCCGCATTGAGGTCACATTGCTTGAACCGGATGCGCAGGGCAATTGGTCCGCACTGATCAAGCCGCTCAAGAAGGTGAA
AATCGCAGAGGAGATCATCTTTTCCGACCGGTTGCGCGCGACCCTTCGCGCAGTCAAAGACGGGCAGGGTGTGTTGCAGT
TCAACCTTGAAGGCGATGATTTCGACGCAGCGCTCAACGCCGCCGGTGCCATGCCGCTGCCACCCTATATCGCCACACGT
CGTCCGGCGGATGAGCGGGACAAATCGGATTACCAGACCGTATTCGCCCAAAACAGTGGGGCCGTGGCTGCGCCGACGGC
CTCGCTGCATTTTGACAAAGCGCTTCTGTCCGAACTGGCGGCGCGCGGGGTGCTGTTCAGCTATGTGACCCTGCACGTGG
GGGCGGGCACATTCCTGCCGGTGAAGGTCGAGGATGTGACAACCCACAAGATGCACGCGGAATGGGGGCAGGTGAGTGCT
GCGGCTGCGGCAGAGATCAACGCGACGAAACAGGCCGGCAAACGGGTGATCCCGGTGGGCACCACGGCGCTGCGTTTGAT
TGAAACCGCCGGGCGCGAGGGTCAGATCAAGGCCTGGGAAGGCGATACCGATATCTTCATCTACCCCGGCTTCCGCTTCA
ACGTCGCGGATGCCCTGATGACGAATTTCCATCTGCCCAAATCCACCCTCATGATGCTGGTCTCTGCGCTGATGGGATAT
GACGAGGTGCGTGCGATCTACGATCACGCGGTCGCCGAAAAATACCGGTTTTTTTCCTACGGCGACGCGTCACTTTTAAT
CCCGCAGCGCGACTGA

Upstream 100 bases:

>100_bases
CGCGCCAAGGCACCGCTGGAGGCGGGCGAACCCGTACCGGTGCCTGAACCGCGCCGCACCCCGGTTGTGACGCGGGGCGA
AGACCGCTAAACGGGCGCAC

Downstream 100 bases:

>100_bases
CGTCACATTACGACGCAATAGAGTCGTAGCTGAGCGGTTTTCGCAGGATGCGATACTGCAAGACGCCGCCAAGAGAGTTT
TTGCCGCGCGCTTTGGAGTC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKLSDFDFDLPEDLIATRPAVPRTSARLLVAKGRQISDSIVSDLPNWLSPGDRLVLNDTRVIPARLTGARHRDTAQGPMQ
ARIEVTLLEPDAQGNWSALIKPLKKVKIAEEIIFSDRLRATLRAVKDGQGVLQFNLEGDDFDAALNAAGAMPLPPYIATR
RPADERDKSDYQTVFAQNSGAVAAPTASLHFDKALLSELAARGVLFSYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVSA
AAAAEINATKQAGKRVIPVGTTALRLIETAGREGQIKAWEGDTDIFIYPGFRFNVADALMTNFHLPKSTLMMLVSALMGY
DEVRAIYDHAVAEKYRFFSYGDASLLIPQRD

Sequences:

>Translated_351_residues
MKLSDFDFDLPEDLIATRPAVPRTSARLLVAKGRQISDSIVSDLPNWLSPGDRLVLNDTRVIPARLTGARHRDTAQGPMQ
ARIEVTLLEPDAQGNWSALIKPLKKVKIAEEIIFSDRLRATLRAVKDGQGVLQFNLEGDDFDAALNAAGAMPLPPYIATR
RPADERDKSDYQTVFAQNSGAVAAPTASLHFDKALLSELAARGVLFSYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVSA
AAAAEINATKQAGKRVIPVGTTALRLIETAGREGQIKAWEGDTDIFIYPGFRFNVADALMTNFHLPKSTLMMLVSALMGY
DEVRAIYDHAVAEKYRFFSYGDASLLIPQRD
>Mature_351_residues
MKLSDFDFDLPEDLIATRPAVPRTSARLLVAKGRQISDSIVSDLPNWLSPGDRLVLNDTRVIPARLTGARHRDTAQGPMQ
ARIEVTLLEPDAQGNWSALIKPLKKVKIAEEIIFSDRLRATLRAVKDGQGVLQFNLEGDDFDAALNAAGAMPLPPYIATR
RPADERDKSDYQTVFAQNSGAVAAPTASLHFDKALLSELAARGVLFSYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVSA
AAAAEINATKQAGKRVIPVGTTALRLIETAGREGQIKAWEGDTDIFIYPGFRFNVADALMTNFHLPKSTLMMLVSALMGY
DEVRAIYDHAVAEKYRFFSYGDASLLIPQRD

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=45.8923512747875, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ROSDO (Q164S6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683203.1
- ProteinModelPortal:   Q164S6
- SMR:   Q164S6
- STRING:   Q164S6
- GeneID:   4195217
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2998
- NMPDR:   fig|375451.6.peg.2789
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   KARIHEA
- PhylomeDB:   Q164S6
- ProtClustDB:   PRK00147
- BioCyc:   RDEN375451:RD1_2998-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38444; Mature: 38444

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDFDFDLPEDLIATRPAVPRTSARLLVAKGRQISDSIVSDLPNWLSPGDRLVLNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCHHHHCCCCEEEEECCE
VIPARLTGARHRDTAQGPMQARIEVTLLEPDAQGNWSALIKPLKKVKIAEEIIFSDRLRA
EEEHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAVKDGQGVLQFNLEGDDFDAALNAAGAMPLPPYIATRRPADERDKSDYQTVFAQNSG
HHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCHHHEEEECCCC
AVAAPTASLHFDKALLSELAARGVLFSYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVSA
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHCCHHH
AAAAEINATKQAGKRVIPVGTTALRLIETAGREGQIKAWEGDTDIFIYPGFRFNVADALM
HHHHHHHHHHHHCCEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEECHHHHHH
TNFHLPKSTLMMLVSALMGYDEVRAIYDHAVAEKYRFFSYGDASLLIPQRD
HHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCC
>Mature Secondary Structure
MKLSDFDFDLPEDLIATRPAVPRTSARLLVAKGRQISDSIVSDLPNWLSPGDRLVLNDTR
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCHHHHCCCCEEEEECCE
VIPARLTGARHRDTAQGPMQARIEVTLLEPDAQGNWSALIKPLKKVKIAEEIIFSDRLRA
EEEHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAVKDGQGVLQFNLEGDDFDAALNAAGAMPLPPYIATRRPADERDKSDYQTVFAQNSG
HHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCHHHEEEECCCC
AVAAPTASLHFDKALLSELAARGVLFSYVTLHVGAGTFLPVKVEDVTTHKMHAEWGQVSA
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHCCHHH
AAAAEINATKQAGKRVIPVGTTALRLIETAGREGQIKAWEGDTDIFIYPGFRFNVADALM
HHHHHHHHHHHHCCEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEECHHHHHH
TNFHLPKSTLMMLVSALMGYDEVRAIYDHAVAEKYRFFSYGDASLLIPQRD
HHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA