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The map label for this gene is cysI [H]

Identifier: 110680165

GI number: 110680165

Start: 2843155

End: 2844819

Strand: Direct

Name: cysI [H]

Synonym: RD1_2966

Alternate gene names: 110680165

Gene position: 2843155-2844819 (Clockwise)

Preceding gene: 110680164

Following gene: 110680166

Centisome position: 68.79

GC content: 60.84

Gene sequence:

>1665_bases
ATGTATAAGTACACCGATTTCGACGCGGCTTTCATCAAGGAGCGCAACGCCCAGTTCCGCAGCCAGGTCGAGCGGCGCAT
TGACGGCTCGCTTACCGAAGATGAATTCAAGCCCCTGCGCCTGATGAACGGGCTGTATCTGCAACTGCATGCTTACATGC
TGCGGGTCGCCATTCCCTATGGGACGCTGAACGGGCGTCAAATGGACAAGCTGGCCGATATCGCCGATCGCTGGGACAAG
GGCTATGGCCATTTCACCACGCGGCAGAACATCCAGTTCAACTGGCCTGAATTGCGGGACATCCCTGATATGCTGGACGC
GCTCGCGGAGGTGCAGATGCATGCGATCCAGACGTCAGGCAACACCATTCGCAACGTGACTGCGGATCATTTCGCCGGGG
CCGCCGCCGATGAGATCGCCGATCCGCGCCCGGTGGCGGAGCTGATCCGGCAATGGTCCACCGACCATCCGGAGTTCCAG
TTCCTGCCCCGCAAGTTCAAAGTCGCCGTGACCGGATCGCCCAATGACCGCGCCGTGACCAAGGCACATGACATCGGGTT
GCGCATGCTGGAACGCGATGGCGTGGCCGGGTTCGAGGTTATCGTGGGCGGTGGCCTCGGGCGCACCCCCATGATCGGCA
AGGTGCTCAGGGATTTCCTGCCGCGCGAAGACCTGCTGCCCTATCTCGAAGCCATCGTGAGCGTCTACAACCTGCTGGGA
CGGCGTGACAACAAGTACAAGGCCCGCATCAAGATCACCGTGCATGAAAACGGCATCGACGATTTCCGCGCGCGGGTAGA
GGAACGCTTTGCGCTGATCCGTCCGCAGTTCGGCGGCATTGATCAACAGCTTCTGGCCGGCATCGAGGCGGATTTCGCAG
CCCCCGATTTCAAAACAAAGCCAGTGGCGGATTTCGAGGCTGCCTATACCAATGATCCGGTCTTCCGGTCCTGGGCCGAC
ACCAACCTGACGGACCACCGCGCCCCGGGCTATGCGATTGTTTCGATCAGCCTCAAGGCGCATGCCGCCACCCCCGGCGA
TGCCACCTCAGCGCAGATGCGCGTGATGGCAGACCTTGCGCGCGACTACGGGCATGATGAATTGCGCATCAGCCACGAAC
AAAACGTCATTCTGCCGCATGTGCATAAATCCGACCTGCCCGCGCTGCATGCACGGCTAAAAGAGGCGCAGTTGGCCACG
GCGAACATCGGGCTGATTTCGGACATCATCGCCTGCCCCGGGATGGATTACTGCGCGCTGGCCACGGCCCGTTCGATCCC
CATAGCGCAAGAGATCGCGACCCGGTTTGACGACCTCAAGCTTGAGCATGACATTGGGCCGCTCAAGATCAAGATATCGG
GCTGCATCAATGCCTGCGGTCATCACCACGTCGGCCACATCGGCATTCTGGGCCTTGATCGCGCAGGGGTCGAAAACTAT
CAGATCACGCTGGGGGGCGACGGATCAGAGGACGCGACCATCGGTGAGCGGGCGGGTCCGGGTTTTTCTGCCGACGAAAT
CATCCCAGCGGTGGAACGGATCGTGATGGCCTATCTTGAATTGCGCGACGCACCCGAAGAAACGTTCCTCGAAACCTATC
GCCGCATCGGTCTGGCCCCCTTCAAGGCGGCCCTTTATCCGGAGGTGCAGGCCGATGCCGCTTGA

Upstream 100 bases:

>100_bases
GACACCGCCGGTGGCGTGCCAAAACCCACCCATTTTCGCGAAGACTTCCGCGCGCGCGGCCCGTCCAACTACGCCCATGG
CAAACAAGAGGACCTGAGCA

Downstream 100 bases:

>100_bases
TGAGGCGCAGCCTGTGGACCAGAGCGGCAGGCGCACAGATGAGTTGCAGGCGCTGGTGCAGGAGCTGAACACGCGCCTGC
GCCATCATGGGGCGGTGGAT

Product: sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MYKYTDFDAAFIKERNAQFRSQVERRIDGSLTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNGRQMDKLADIADRWDK
GYGHFTTRQNIQFNWPELRDIPDMLDALAEVQMHAIQTSGNTIRNVTADHFAGAAADEIADPRPVAELIRQWSTDHPEFQ
FLPRKFKVAVTGSPNDRAVTKAHDIGLRMLERDGVAGFEVIVGGGLGRTPMIGKVLRDFLPREDLLPYLEAIVSVYNLLG
RRDNKYKARIKITVHENGIDDFRARVEERFALIRPQFGGIDQQLLAGIEADFAAPDFKTKPVADFEAAYTNDPVFRSWAD
TNLTDHRAPGYAIVSISLKAHAATPGDATSAQMRVMADLARDYGHDELRISHEQNVILPHVHKSDLPALHARLKEAQLAT
ANIGLISDIIACPGMDYCALATARSIPIAQEIATRFDDLKLEHDIGPLKIKISGCINACGHHHVGHIGILGLDRAGVENY
QITLGGDGSEDATIGERAGPGFSADEIIPAVERIVMAYLELRDAPEETFLETYRRIGLAPFKAALYPEVQADAA

Sequences:

>Translated_554_residues
MYKYTDFDAAFIKERNAQFRSQVERRIDGSLTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNGRQMDKLADIADRWDK
GYGHFTTRQNIQFNWPELRDIPDMLDALAEVQMHAIQTSGNTIRNVTADHFAGAAADEIADPRPVAELIRQWSTDHPEFQ
FLPRKFKVAVTGSPNDRAVTKAHDIGLRMLERDGVAGFEVIVGGGLGRTPMIGKVLRDFLPREDLLPYLEAIVSVYNLLG
RRDNKYKARIKITVHENGIDDFRARVEERFALIRPQFGGIDQQLLAGIEADFAAPDFKTKPVADFEAAYTNDPVFRSWAD
TNLTDHRAPGYAIVSISLKAHAATPGDATSAQMRVMADLARDYGHDELRISHEQNVILPHVHKSDLPALHARLKEAQLAT
ANIGLISDIIACPGMDYCALATARSIPIAQEIATRFDDLKLEHDIGPLKIKISGCINACGHHHVGHIGILGLDRAGVENY
QITLGGDGSEDATIGERAGPGFSADEIIPAVERIVMAYLELRDAPEETFLETYRRIGLAPFKAALYPEVQADAA
>Mature_554_residues
MYKYTDFDAAFIKERNAQFRSQVERRIDGSLTEDEFKPLRLMNGLYLQLHAYMLRVAIPYGTLNGRQMDKLADIADRWDK
GYGHFTTRQNIQFNWPELRDIPDMLDALAEVQMHAIQTSGNTIRNVTADHFAGAAADEIADPRPVAELIRQWSTDHPEFQ
FLPRKFKVAVTGSPNDRAVTKAHDIGLRMLERDGVAGFEVIVGGGLGRTPMIGKVLRDFLPREDLLPYLEAIVSVYNLLG
RRDNKYKARIKITVHENGIDDFRARVEERFALIRPQFGGIDQQLLAGIEADFAAPDFKTKPVADFEAAYTNDPVFRSWAD
TNLTDHRAPGYAIVSISLKAHAATPGDATSAQMRVMADLARDYGHDELRISHEQNVILPHVHKSDLPALHARLKEAQLAT
ANIGLISDIIACPGMDYCALATARSIPIAQEIATRFDDLKLEHDIGPLKIKISGCINACGHHHVGHIGILGLDRAGVENY
QITLGGDGSEDATIGERAGPGFSADEIIPAVERIVMAYLELRDAPEETFLETYRRIGLAPFKAALYPEVQADAA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=513, Percent_Identity=23.7816764132554, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322597, Length=583, Percent_Identity=22.4699828473413, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 61557; Mature: 61557

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKYTDFDAAFIKERNAQFRSQVERRIDGSLTEDEFKPLRLMNGLYLQLHAYMLRVAIPY
CCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTLNGRQMDKLADIADRWDKGYGHFTTRQNIQFNWPELRDIPDMLDALAEVQMHAIQTSG
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHHCCC
NTIRNVTADHFAGAAADEIADPRPVAELIRQWSTDHPEFQFLPRKFKVAVTGSPNDRAVT
CEEECCCHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCH
KAHDIGLRMLERDGVAGFEVIVGGGLGRTPMIGKVLRDFLPREDLLPYLEAIVSVYNLLG
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RRDNKYKARIKITVHENGIDDFRARVEERFALIRPQFGGIDQQLLAGIEADFAAPDFKTK
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCC
PVADFEAAYTNDPVFRSWADTNLTDHRAPGYAIVSISLKAHAATPGDATSAQMRVMADLA
CCCCCHHHCCCCCCEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHH
RDYGHDELRISHEQNVILPHVHKSDLPALHARLKEAQLATANIGLISDIIACPGMDYCAL
HHCCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
ATARSIPIAQEIATRFDDLKLEHDIGPLKIKISGCINACGHHHVGHIGILGLDRAGVENY
HHHCCCCHHHHHHHHHHCCEEECCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCE
QITLGGDGSEDATIGERAGPGFSADEIIPAVERIVMAYLELRDAPEETFLETYRRIGLAP
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCH
FKAALYPEVQADAA
HHHHHCCCCCCCCC
>Mature Secondary Structure
MYKYTDFDAAFIKERNAQFRSQVERRIDGSLTEDEFKPLRLMNGLYLQLHAYMLRVAIPY
CCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTLNGRQMDKLADIADRWDKGYGHFTTRQNIQFNWPELRDIPDMLDALAEVQMHAIQTSG
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHHCCC
NTIRNVTADHFAGAAADEIADPRPVAELIRQWSTDHPEFQFLPRKFKVAVTGSPNDRAVT
CEEECCCHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCH
KAHDIGLRMLERDGVAGFEVIVGGGLGRTPMIGKVLRDFLPREDLLPYLEAIVSVYNLLG
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RRDNKYKARIKITVHENGIDDFRARVEERFALIRPQFGGIDQQLLAGIEADFAAPDFKTK
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCC
PVADFEAAYTNDPVFRSWADTNLTDHRAPGYAIVSISLKAHAATPGDATSAQMRVMADLA
CCCCCHHHCCCCCCEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHH
RDYGHDELRISHEQNVILPHVHKSDLPALHARLKEAQLATANIGLISDIIACPGMDYCAL
HHCCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
ATARSIPIAQEIATRFDDLKLEHDIGPLKIKISGCINACGHHHVGHIGILGLDRAGVENY
HHHCCCCHHHHHHHHHHCCEEECCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCE
QITLGGDGSEDATIGERAGPGFSADEIIPAVERIVMAYLELRDAPEETFLETYRRIGLAP
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCH
FKAALYPEVQADAA
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]