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The map label for this gene is glpK

Identifier: 110680087

GI number: 110680087

Start: 2759871

End: 2761358

Strand: Reverse

Name: glpK

Synonym: RD1_2883

Alternate gene names: 110680087

Gene position: 2761358-2759871 (Counterclockwise)

Preceding gene: 110680088

Following gene: 110680086

Centisome position: 66.81

GC content: 60.15

Gene sequence:

>1488_bases
ATGACCCATATCTTGGCCATTGATCAGGGAACCACGTCGTCACGCGCGATCATATTTGATGCGTCGATGAACATCACCGC
ATCCGCGCAGGAGGAGTTCGCGCAACACTATCCCGACAGCGGCTGGGTTGAGCATGATCCCGGCGATTTATGGGCAACCA
CCGCAGGGACCTGCCGCGCGGCCATCGAAAAAGCAGGCTTGAAGCCTGAGGACATCGCCGCCATCGGGATCACAAATCAA
CGCGAAACCGTGGTGGTCTGGGATAAAACATCAGGTCAGCCGGTTTACAACGCGATCGTTTGGCAGGACCGGCGCACGGC
GGCCTATTGCAAGACGCTGCGCGATGAAGGTCATGACAAGATGATCACCGCGCGCACTGGGCTTCTGGCGGATCCGTATT
TCTCGGCGACCAAGCTGAAGTGGATATTGGACAATGTGGAAGGTGCAAGGGACCGCGCAACGCGCGGTGAATTGCTCTTT
GGCACGGTTGATACCTTTCTGATCTGGAAACTGACCGGCGGGGCGGCCCATGTCACGGATGCCACGAATGCCGCGCGCAC
GTCCCTTTATGACATCCACAAAGGGCGCTGGAGCCAGACGATCTGCGCGCTGTTTGATATTCCGCAACAGATGTTGCCGG
AGGTAAAAGACTGCGCGGCGGATTTTGGCGTGACGCGCTCTGATCTGTTTGGCCGCCCCGTGCCAATCCTTGGGGTGGCG
GGCGATCAGCAGGCGGCGACAATCGGGCAGGCGTGTTTTGAACCGGGCATGTTGAAATCGACCTATGGCACGGGGTGTTT
CGCCTTGCTGAATACAGGCGACACGGCAGTGGCTTCGAGCAATCGGTTGCTGACCACGATCGCCTATCAGTTTGATGGCA
AACCGACCTATGCGCTTGAGGGGTCGATTTTCGTCGCCGGGGCTGTGGTGCAATGGCTGCGTGATGGGCTGCAAACCATC
CGCAAGGCCAGCGAAACCCAAGCGATGGCAGAACGCGCGGACCCGAACCAGAATGTGGTGCTGGTGCCTGCATTTGTGGG
CTTGGGCGCGCCCTATTGGGATGCCGAGTGTCGGGGCGCTGTGTTCGGGCTGACGCGAAATTCAGGGCCGGACGAATTTG
CCAGGGCCGCGCTGGAGAGCGTCGGATACCAAACCCGCGATCTGTTGGATGCGATGACAGCGGATTGGCAGGGCAGTGAC
GTGCGTGCAACGCTGCGGGTGGATGGGGGCATGTCAGCCTCGGACTGGGCGATGCAGTTCCTGTCTGACATTATCGATGC
GCCGGTGGATCGCCCCAAAGTGCTTGAAACAACCGCCTTGGGGGCGGCGTGGCTGGCGGGGCAACGTGCGGGCCTATATC
CTGATCAGGCCGGATTTGCCGCAAGCTGGGCTCTTGAAAAGACCTTTGCGCCGCAGATGGACGCGGCTCTGCGCGACAGG
AAATACGCAGCCTGGAAGCGGGCGGTGGATGCCACCCTGCGCTTTTGA

Upstream 100 bases:

>100_bases
CGCATGAGTCCGCAGGAAGTTTCTGTTCTGGATGACTTTATGCAGGCACACAGGCTAAAAGAAGGCTCTGACGCTGCACA
ATAACTTGGGGGAGGGGTGC

Downstream 100 bases:

>100_bases
GAACGGCATGGGGTCAGCCACATTCACTTTTCAAACCGCGGGCCGGGTCACTTTTGGGCGCGGTGCGCGCGTATCAGCGG
CGCAGGCGGTTGCGGCGTTC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTHILAIDQGTTSSRAIIFDASMNITASAQEEFAQHYPDSGWVEHDPGDLWATTAGTCRAAIEKAGLKPEDIAAIGITNQ
RETVVVWDKTSGQPVYNAIVWQDRRTAAYCKTLRDEGHDKMITARTGLLADPYFSATKLKWILDNVEGARDRATRGELLF
GTVDTFLIWKLTGGAAHVTDATNAARTSLYDIHKGRWSQTICALFDIPQQMLPEVKDCAADFGVTRSDLFGRPVPILGVA
GDQQAATIGQACFEPGMLKSTYGTGCFALLNTGDTAVASSNRLLTTIAYQFDGKPTYALEGSIFVAGAVVQWLRDGLQTI
RKASETQAMAERADPNQNVVLVPAFVGLGAPYWDAECRGAVFGLTRNSGPDEFARAALESVGYQTRDLLDAMTADWQGSD
VRATLRVDGGMSASDWAMQFLSDIIDAPVDRPKVLETTALGAAWLAGQRAGLYPDQAGFAASWALEKTFAPQMDAALRDR
KYAAWKRAVDATLRF

Sequences:

>Translated_495_residues
MTHILAIDQGTTSSRAIIFDASMNITASAQEEFAQHYPDSGWVEHDPGDLWATTAGTCRAAIEKAGLKPEDIAAIGITNQ
RETVVVWDKTSGQPVYNAIVWQDRRTAAYCKTLRDEGHDKMITARTGLLADPYFSATKLKWILDNVEGARDRATRGELLF
GTVDTFLIWKLTGGAAHVTDATNAARTSLYDIHKGRWSQTICALFDIPQQMLPEVKDCAADFGVTRSDLFGRPVPILGVA
GDQQAATIGQACFEPGMLKSTYGTGCFALLNTGDTAVASSNRLLTTIAYQFDGKPTYALEGSIFVAGAVVQWLRDGLQTI
RKASETQAMAERADPNQNVVLVPAFVGLGAPYWDAECRGAVFGLTRNSGPDEFARAALESVGYQTRDLLDAMTADWQGSD
VRATLRVDGGMSASDWAMQFLSDIIDAPVDRPKVLETTALGAAWLAGQRAGLYPDQAGFAASWALEKTFAPQMDAALRDR
KYAAWKRAVDATLRF
>Mature_494_residues
THILAIDQGTTSSRAIIFDASMNITASAQEEFAQHYPDSGWVEHDPGDLWATTAGTCRAAIEKAGLKPEDIAAIGITNQR
ETVVVWDKTSGQPVYNAIVWQDRRTAAYCKTLRDEGHDKMITARTGLLADPYFSATKLKWILDNVEGARDRATRGELLFG
TVDTFLIWKLTGGAAHVTDATNAARTSLYDIHKGRWSQTICALFDIPQQMLPEVKDCAADFGVTRSDLFGRPVPILGVAG
DQQAATIGQACFEPGMLKSTYGTGCFALLNTGDTAVASSNRLLTTIAYQFDGKPTYALEGSIFVAGAVVQWLRDGLQTIR
KASETQAMAERADPNQNVVLVPAFVGLGAPYWDAECRGAVFGLTRNSGPDEFARAALESVGYQTRDLLDAMTADWQGSDV
RATLRVDGGMSASDWAMQFLSDIIDAPVDRPKVLETTALGAAWLAGQRAGLYPDQAGFAASWALEKTFAPQMDAALRDRK
YAAWKRAVDATLRF

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=41.7322834645669, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI4504007, Length=508, Percent_Identity=41.7322834645669, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=41.3255360623782, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI41393575, Length=509, Percent_Identity=41.2573673870334, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=30.3747534516765, Blast_Score=275, Evalue=6e-74,
Organism=Escherichia coli, GI1790361, Length=489, Percent_Identity=52.760736196319, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789987, Length=501, Percent_Identity=26.1477045908184, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787789, Length=484, Percent_Identity=25, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790005, Length=507, Percent_Identity=23.2741617357002, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789168, Length=458, Percent_Identity=25.3275109170306, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=40.5138339920949, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=23.9382239382239, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=24.8322147651007, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321755, Length=476, Percent_Identity=36.5546218487395, Blast_Score=270, Evalue=5e-73,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=40.5138339920949, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24654573, Length=451, Percent_Identity=40.7982261640798, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=36.6533864541833, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=36.6533864541833, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=36.6533864541833, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=36.6533864541833, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=36.6533864541833, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=31.2380952380952, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=31.8458417849899, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24656367, Length=494, Percent_Identity=30.5668016194332, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21357867, Length=507, Percent_Identity=29.7830374753452, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656372, Length=339, Percent_Identity=31.858407079646, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI45552909, Length=258, Percent_Identity=32.5581395348837, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_ROSDO (Q165D5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683094.1
- ProteinModelPortal:   Q165D5
- SMR:   Q165D5
- STRING:   Q165D5
- GeneID:   4196040
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2883
- NMPDR:   fig|375451.6.peg.2678
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- PhylomeDB:   Q165D5
- ProtClustDB:   CLSK956447
- BioCyc:   RDEN375451:RD1_2883-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53686; Mature: 53555

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHILAIDQGTTSSRAIIFDASMNITASAQEEFAQHYPDSGWVEHDPGDLWATTAGTCRA
CCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCEECCCCCEEECCCHHHHH
AIEKAGLKPEDIAAIGITNQRETVVVWDKTSGQPVYNAIVWQDRRTAAYCKTLRDEGHDK
HHHHCCCCHHHEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCE
MITARTGLLADPYFSATKLKWILDNVEGARDRATRGELLFGTVDTFLIWKLTGGAAHVTD
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEEECCCCEEECC
ATNAARTSLYDIHKGRWSQTICALFDIPQQMLPEVKDCAADFGVTRSDLFGRPVPILGVA
CCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEC
GDQQAATIGQACFEPGMLKSTYGTGCFALLNTGDTAVASSNRLLTTIAYQFDGKPTYALE
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEC
GSIFVAGAVVQWLRDGLQTIRKASETQAMAERADPNQNVVLVPAFVGLGAPYWDAECRGA
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCE
VFGLTRNSGPDEFARAALESVGYQTRDLLDAMTADWQGSDVRATLRVDGGMSASDWAMQF
EEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
LSDIIDAPVDRPKVLETTALGAAWLAGQRAGLYPDQAGFAASWALEKTFAPQMDAALRDR
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KYAAWKRAVDATLRF
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
THILAIDQGTTSSRAIIFDASMNITASAQEEFAQHYPDSGWVEHDPGDLWATTAGTCRA
CEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCEECCCCCEEECCCHHHHH
AIEKAGLKPEDIAAIGITNQRETVVVWDKTSGQPVYNAIVWQDRRTAAYCKTLRDEGHDK
HHHHCCCCHHHEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCE
MITARTGLLADPYFSATKLKWILDNVEGARDRATRGELLFGTVDTFLIWKLTGGAAHVTD
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEEECCCCEEECC
ATNAARTSLYDIHKGRWSQTICALFDIPQQMLPEVKDCAADFGVTRSDLFGRPVPILGVA
CCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEC
GDQQAATIGQACFEPGMLKSTYGTGCFALLNTGDTAVASSNRLLTTIAYQFDGKPTYALE
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEC
GSIFVAGAVVQWLRDGLQTIRKASETQAMAERADPNQNVVLVPAFVGLGAPYWDAECRGA
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCE
VFGLTRNSGPDEFARAALESVGYQTRDLLDAMTADWQGSDVRATLRVDGGMSASDWAMQF
EEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
LSDIIDAPVDRPKVLETTALGAAWLAGQRAGLYPDQAGFAASWALEKTFAPQMDAALRDR
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KYAAWKRAVDATLRF
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA