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The map label for this gene is dapE [H]

Identifier: 110680035

GI number: 110680035

Start: 2699808

End: 2701178

Strand: Direct

Name: dapE [H]

Synonym: RD1_2828

Alternate gene names: 110680035

Gene position: 2699808-2701178 (Clockwise)

Preceding gene: 110680031

Following gene: 110680036

Centisome position: 65.32

GC content: 59.96

Gene sequence:

>1371_bases
ATGTCGTTGGACGATGTCTTGTCCCGTCTCGATGCGGAAATGCCCAAAGCCACGGAACGGCTTTTCGAACTGCTGCGCAT
TGCGTCAATTTCAACCGATCCCGCTTACAAGCACGACTGTGAGCGCGCCGCGGACTGGCTGGTGAATGACCTGCAAAGCA
TGGGTGTCGCGGCCGAAAAACGCAGCACACCGGGGCATCCGATGGTGGTTGGCCACGCGGACGGACCCGGCCCGCATCTG
CTGTTTTACGGGCATTACGACGTGCAACCGGTTGATCCGATTGAACTTTGGGACAATGACCCATTCGATCCGAAACTTGA
ACAAACCGACAAAGGCCCGGTGCTGCGCGGGCGCGGCACGGCGGATGACAAGGGCCAGTTGATGACCTTTCTTGAGGCCT
GCCGCGCGTGGAAGGCGGAACACGGCAGTTTGCCCTGCAAGATCACCTTCTTTTTTGAAGGCGAGGAGGAAAGCGGCTCG
CCCTCGCTCGTGCCCTTCATGAAGGAAAACAGCGACACGTTGCGCGCCGATCTGGCGCTGATCTGTGACACGGCGATGTT
TGAAAGCAAAACGCCCGCGATCGTCACGATGCTGCGCGGGATGATGTCGGATGAGATCACCATCACCGGGCCGGACAAGG
ACCTGCATTCGGGCTATTACGGCGGCATCGCGATGAACCCCATCCGCGTGCTGTCGCGTGTCATCGCCGCCTTGCATGAT
GACACGGGTCGCGTCACCATCCCCGGATTTTATGACGGTGTCCCCGATCTGAGCGCAGACATGCAGGCGCAATGGGCCGG
GTTGGGCTTTGATCATACCCAGTTTCTGGGTGACGTCGATCTTTCTGTCCCTGCCGGTGAGGCAGGTGTGACACCGCTTG
AGATGACGTGGTCCCGCCCCACCTGCGAGGTCAACGGCATCTGGGGTGGCTATATCGGTGAAGGGTTCAAGACCGTCCTG
CCCTCGCAAGCGCATGCGAAAATCAGCTTTCGGCTGGTCGGGGATCAGGACCCGCACGCGATCCGCGAGAACTTTCGCGC
CATGGTCCAAAACCTCCTGCCGCCCGATTGCACCGCCAGCTTCAAGGATCACGGGGCCAGTAAGGCCTGCGTCATTGACG
CCGAAGACCCCCGGTTTGACAAAGCGCTCGCGGCGCTGAGCGAAGAATGGGGCGTGCCCGCGGCCTATATCGGCGGGGGC
GGCTCGATACCGATTGCCGGGCATTTTCAGGACATCCTGAACACAAAACCCCTGCTGATCGGATTCGGCAAGGATGATGA
TGCGATACATTCACCGAACGAGAAATATGACCTTGAGAGCTTTCACAAAGGTGCGCGCAGCTGGGCGCGAATCCTCGCGG
CGCTGACCTGA

Upstream 100 bases:

>100_bases
GGACGCCATGTCGCAGGAGCACCCTTTGCGCAGCGCCGCGATCTGGACAGCATCCGCCTGCCTGCTAGGGTGCGCATCAG
TCAATTCAGGAGAACACCTC

Downstream 100 bases:

>100_bases
TGTTTGCCGGGTTCGAGGTGGGCGACGCCTTGGTCAACGGACAGTCCGTCCACTATCGCGCTGGTGGGCAAGGCGCTCCG
GTGCTGTTGTTGCATGGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSLDDVLSRLDAEMPKATERLFELLRIASISTDPAYKHDCERAADWLVNDLQSMGVAAEKRSTPGHPMVVGHADGPGPHL
LFYGHYDVQPVDPIELWDNDPFDPKLEQTDKGPVLRGRGTADDKGQLMTFLEACRAWKAEHGSLPCKITFFFEGEEESGS
PSLVPFMKENSDTLRADLALICDTAMFESKTPAIVTMLRGMMSDEITITGPDKDLHSGYYGGIAMNPIRVLSRVIAALHD
DTGRVTIPGFYDGVPDLSADMQAQWAGLGFDHTQFLGDVDLSVPAGEAGVTPLEMTWSRPTCEVNGIWGGYIGEGFKTVL
PSQAHAKISFRLVGDQDPHAIRENFRAMVQNLLPPDCTASFKDHGASKACVIDAEDPRFDKALAALSEEWGVPAAYIGGG
GSIPIAGHFQDILNTKPLLIGFGKDDDAIHSPNEKYDLESFHKGARSWARILAALT

Sequences:

>Translated_456_residues
MSLDDVLSRLDAEMPKATERLFELLRIASISTDPAYKHDCERAADWLVNDLQSMGVAAEKRSTPGHPMVVGHADGPGPHL
LFYGHYDVQPVDPIELWDNDPFDPKLEQTDKGPVLRGRGTADDKGQLMTFLEACRAWKAEHGSLPCKITFFFEGEEESGS
PSLVPFMKENSDTLRADLALICDTAMFESKTPAIVTMLRGMMSDEITITGPDKDLHSGYYGGIAMNPIRVLSRVIAALHD
DTGRVTIPGFYDGVPDLSADMQAQWAGLGFDHTQFLGDVDLSVPAGEAGVTPLEMTWSRPTCEVNGIWGGYIGEGFKTVL
PSQAHAKISFRLVGDQDPHAIRENFRAMVQNLLPPDCTASFKDHGASKACVIDAEDPRFDKALAALSEEWGVPAAYIGGG
GSIPIAGHFQDILNTKPLLIGFGKDDDAIHSPNEKYDLESFHKGARSWARILAALT
>Mature_455_residues
SLDDVLSRLDAEMPKATERLFELLRIASISTDPAYKHDCERAADWLVNDLQSMGVAAEKRSTPGHPMVVGHADGPGPHLL
FYGHYDVQPVDPIELWDNDPFDPKLEQTDKGPVLRGRGTADDKGQLMTFLEACRAWKAEHGSLPCKITFFFEGEEESGSP
SLVPFMKENSDTLRADLALICDTAMFESKTPAIVTMLRGMMSDEITITGPDKDLHSGYYGGIAMNPIRVLSRVIAALHDD
TGRVTIPGFYDGVPDLSADMQAQWAGLGFDHTQFLGDVDLSVPAGEAGVTPLEMTWSRPTCEVNGIWGGYIGEGFKTVLP
SQAHAKISFRLVGDQDPHAIRENFRAMVQNLLPPDCTASFKDHGASKACVIDAEDPRFDKALAALSEEWGVPAAYIGGGG
SIPIAGHFQDILNTKPLLIGFGKDDDAIHSPNEKYDLESFHKGARSWARILAALT

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=474, Percent_Identity=27.2151898734177, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI21071039, Length=475, Percent_Identity=26.5263157894737, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI271398277, Length=319, Percent_Identity=29.153605015674, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17563146, Length=465, Percent_Identity=31.6129032258064, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI193211059, Length=471, Percent_Identity=27.6008492569002, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=479, Percent_Identity=29.4363256784969, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319758, Length=465, Percent_Identity=23.2258064516129, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24585880, Length=445, Percent_Identity=28.314606741573, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49697; Mature: 49565

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDDVLSRLDAEMPKATERLFELLRIASISTDPAYKHDCERAADWLVNDLQSMGVAAEK
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHC
RSTPGHPMVVGHADGPGPHLLFYGHYDVQPVDPIELWDNDPFDPKLEQTDKGPVLRGRGT
CCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEECCCC
ADDKGQLMTFLEACRAWKAEHGSLPCKITFFFEGEEESGSPSLVPFMKENSDTLRADLAL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHH
ICDTAMFESKTPAIVTMLRGMMSDEITITGPDKDLHSGYYGGIAMNPIRVLSRVIAALHD
HHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCHHCCCCCCCEECCHHHHHHHHHHHHHC
DTGRVTIPGFYDGVPDLSADMQAQWAGLGFDHTQFLGDVDLSVPAGEAGVTPLEMTWSRP
CCCCEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCC
TCEVNGIWGGYIGEGFKTVLPSQAHAKISFRLVGDQDPHAIRENFRAMVQNLLPPDCTAS
CEEECCCCCCHHCCCHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
FKDHGASKACVIDAEDPRFDKALAALSEEWGVPAAYIGGGGSIPIAGHFQDILNTKPLLI
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCEEE
GFGKDDDAIHSPNEKYDLESFHKGARSWARILAALT
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLDDVLSRLDAEMPKATERLFELLRIASISTDPAYKHDCERAADWLVNDLQSMGVAAEK
CHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHC
RSTPGHPMVVGHADGPGPHLLFYGHYDVQPVDPIELWDNDPFDPKLEQTDKGPVLRGRGT
CCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEECCCC
ADDKGQLMTFLEACRAWKAEHGSLPCKITFFFEGEEESGSPSLVPFMKENSDTLRADLAL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHH
ICDTAMFESKTPAIVTMLRGMMSDEITITGPDKDLHSGYYGGIAMNPIRVLSRVIAALHD
HHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCHHCCCCCCCEECCHHHHHHHHHHHHHC
DTGRVTIPGFYDGVPDLSADMQAQWAGLGFDHTQFLGDVDLSVPAGEAGVTPLEMTWSRP
CCCCEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCC
TCEVNGIWGGYIGEGFKTVLPSQAHAKISFRLVGDQDPHAIRENFRAMVQNLLPPDCTAS
CEEECCCCCCHHCCCHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
FKDHGASKACVIDAEDPRFDKALAALSEEWGVPAAYIGGGGSIPIAGHFQDILNTKPLLI
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCEEE
GFGKDDDAIHSPNEKYDLESFHKGARSWARILAALT
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA