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The map label for this gene is ilvB [H]

Identifier: 110680019

GI number: 110680019

Start: 2680972

End: 2682723

Strand: Direct

Name: ilvB [H]

Synonym: RD1_2812

Alternate gene names: 110680019

Gene position: 2680972-2682723 (Clockwise)

Preceding gene: 110680018

Following gene: 110680020

Centisome position: 64.87

GC content: 59.36

Gene sequence:

>1752_bases
ATGACACGTCAAATGTCCGGCGCGAAAATGATTGTTCAGGCCCTGATTGATCAGGGCGTGGATACCGTCTTTGGATATCC
CGGCGGGGCTGTGCTGCCGATTTATGACGAGGTCTTTCAACAAAATGCGATCCGCCACATTCTGGTGCGCCACGAACAGG
GTGCGGTACACGCCGCCGAAGGCTATGCGCGCGCCACGGGCAAGCCAGGCGTTGTTCTGGTGACATCTGGTCCGGGGGCA
ACCAATGCCGTGACCGGCCTGACGGATGCGCTGATGGATTCCATTCCGCTGGTGGTTCTGACCGGACAGGTGCCGACTTT
CATGATCGGCTCTGACGCGTTTCAGGAGGCCGATACCGTGGGCATCACCCGCCCCTGCACCAAGCACAACTGGCTGGTCA
AGGAAACCGAACGGCTCTCCGGCGTCATCCACGAAGCGTTTCACGTCGCAACCAGCGGGCGCCCCGGCCCCGTGCTGGTC
GACATTCCCAAGGATGTGCAATTTGCCACCGGCACCTATACGCCGCCCAAACCTTCCACCTCGCATTACCAGCCCAAGCT
GAAGGGTGACATGGAGGCGATCACCGAACTGGTGGCCGCGCTCGAAAAGGCAAAGAAGCCGATTTTCTACACCGGCGGTG
GTGTGATCAATTCCGGCCCGGCCGCCAGCCAGTTGCTGCGTGAACTGGTGCAGGCCACAGGCTTTCCGATCACCTCCACG
CTAATGGGGCTTGGTGCCTATCCGGCCTCGGGGGAAAACTGGATCGGCATGTTGGGCATGCATGGCCTTTATGAAGCCAA
TATGGCGATGCATGATTGCGACCTGATGATCAACATCGGCGCGCGCTTTGATGACCGGATCACCGGGCGGCTCGATGCCT
TCAGCCCGAAATCCATCAAGGCGCATATTGATATCGACCCCTCGTCGATCAACAAGGTCATCAAGACCGACATCCCCGTT
GTCGGGGACATCGCCCATGTGCTTGAGGATGTCCTCAAGGTCTGGAAATCCCGCGGGCGTAAAACGAATTCGGAGGCGGT
CGCCAAATGGTGGGCGCAGATCGAGCAATGGAAAACGGTGGATTGTCTGCGCTTTACGCAAGAGGGCAAGGTCATCAAAC
CGCAACACGCTCTGGCCCGCCTCGAAGCGCTGACCAAGAAATACGACCGCTACATCTGCACCGAAGTCGGGCAGCACCAG
ATGTGGGCCGCGCAATATCTGGGCTTTGAGGATCCGAACCGCTGGATGACCTCCGGTGGTCTGGGCACGATGGGCTATGG
CTTCCCGGCCTCGATCGGCGTTCAGATCGCCCATCCGGATGCGCTGGTGATCAACGTGGCGGGCGAAGCGTCGTGGCTGA
TGAACATGCAGGAGATGGGCACCGCCGTGCAATACCGCCTGCCCGTCAAACAGTTCATCCTCAACAACGAACGCCTCGGT
ATGGTGCGCCAGTGGCAGGAACTGCTGCACGGGGAGCGCTATTCACACAGCTGGTCCGACGCCCTGCCCGATTTCGTAAA
ACTCGCCGAAGCCTTCGGAGCCAAAGGCATCCTGTGTTCCGACCCCGATGATCTGGACGATGCGATCATGGAGATGCTGA
CCCATGACGGCCCTGTCATCTTTGACTGTCTGGTGACAAAGCACGAAAACTGCTTCCCCATGATCCCGTCGGGCAAAGCG
CATAACGAGATGCTGCTCGGCGAAGCGGATACACAGGGCGTGATCGACGCCAAGGGGAGTGTGTTGGTGTGA

Upstream 100 bases:

>100_bases
CGGTAGACCATAGTCTGACCCATGCGCCCCCGCCAAGACCGGGGGCTTTTTTGTGCGCAAAAGATAACGGGCCCATCGGG
GTCAACACGGAGAGAAAACT

Downstream 100 bases:

>100_bases
ATTCAGATAAACGTCACTCGCTGGGCCACACTATGCTAATGCCAAAGTGGTGGCAGCTATTCGTGATGGTATTCTTTTCG
TTGTGCAGCTTGCTTTGGGT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MTRQMSGAKMIVQALIDQGVDTVFGYPGGAVLPIYDEVFQQNAIRHILVRHEQGAVHAAEGYARATGKPGVVLVTSGPGA
TNAVTGLTDALMDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKETERLSGVIHEAFHVATSGRPGPVLV
DIPKDVQFATGTYTPPKPSTSHYQPKLKGDMEAITELVAALEKAKKPIFYTGGGVINSGPAASQLLRELVQATGFPITST
LMGLGAYPASGENWIGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRLDAFSPKSIKAHIDIDPSSINKVIKTDIPV
VGDIAHVLEDVLKVWKSRGRKTNSEAVAKWWAQIEQWKTVDCLRFTQEGKVIKPQHALARLEALTKKYDRYICTEVGQHQ
MWAAQYLGFEDPNRWMTSGGLGTMGYGFPASIGVQIAHPDALVINVAGEASWLMNMQEMGTAVQYRLPVKQFILNNERLG
MVRQWQELLHGERYSHSWSDALPDFVKLAEAFGAKGILCSDPDDLDDAIMEMLTHDGPVIFDCLVTKHENCFPMIPSGKA
HNEMLLGEADTQGVIDAKGSVLV

Sequences:

>Translated_583_residues
MTRQMSGAKMIVQALIDQGVDTVFGYPGGAVLPIYDEVFQQNAIRHILVRHEQGAVHAAEGYARATGKPGVVLVTSGPGA
TNAVTGLTDALMDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKETERLSGVIHEAFHVATSGRPGPVLV
DIPKDVQFATGTYTPPKPSTSHYQPKLKGDMEAITELVAALEKAKKPIFYTGGGVINSGPAASQLLRELVQATGFPITST
LMGLGAYPASGENWIGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRLDAFSPKSIKAHIDIDPSSINKVIKTDIPV
VGDIAHVLEDVLKVWKSRGRKTNSEAVAKWWAQIEQWKTVDCLRFTQEGKVIKPQHALARLEALTKKYDRYICTEVGQHQ
MWAAQYLGFEDPNRWMTSGGLGTMGYGFPASIGVQIAHPDALVINVAGEASWLMNMQEMGTAVQYRLPVKQFILNNERLG
MVRQWQELLHGERYSHSWSDALPDFVKLAEAFGAKGILCSDPDDLDDAIMEMLTHDGPVIFDCLVTKHENCFPMIPSGKA
HNEMLLGEADTQGVIDAKGSVLV
>Mature_582_residues
TRQMSGAKMIVQALIDQGVDTVFGYPGGAVLPIYDEVFQQNAIRHILVRHEQGAVHAAEGYARATGKPGVVLVTSGPGAT
NAVTGLTDALMDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKETERLSGVIHEAFHVATSGRPGPVLVD
IPKDVQFATGTYTPPKPSTSHYQPKLKGDMEAITELVAALEKAKKPIFYTGGGVINSGPAASQLLRELVQATGFPITSTL
MGLGAYPASGENWIGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRLDAFSPKSIKAHIDIDPSSINKVIKTDIPVV
GDIAHVLEDVLKVWKSRGRKTNSEAVAKWWAQIEQWKTVDCLRFTQEGKVIKPQHALARLEALTKKYDRYICTEVGQHQM
WAAQYLGFEDPNRWMTSGGLGTMGYGFPASIGVQIAHPDALVINVAGEASWLMNMQEMGTAVQYRLPVKQFILNNERLGM
VRQWQELLHGERYSHSWSDALPDFVKLAEAFGAKGILCSDPDDLDDAIMEMLTHDGPVIFDCLVTKHENCFPMIPSGKAH
NEMLLGEADTQGVIDAKGSVLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=546, Percent_Identity=25.6410256410256, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=24.8322147651007, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=49.4755244755245, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=40.3197158081705, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=573, Percent_Identity=33.1588132635253, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=27.536231884058, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=24.6478873239437, Blast_Score=139, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=24.9557522123894, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=24.9557522123894, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=26.3461538461538, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=45.4700854700855, Blast_Score=493, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320816, Length=500, Percent_Identity=23.6, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=540, Percent_Identity=22.7777777777778, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323163, Length=593, Percent_Identity=21.9224283305228, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=24.7368421052632, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63573; Mature: 63442

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQMSGAKMIVQALIDQGVDTVFGYPGGAVLPIYDEVFQQNAIRHILVRHEQGAVHAAE
CCCCCCHHHHHHHHHHHCCCHHEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCC
GYARATGKPGVVLVTSGPGATNAVTGLTDALMDSIPLVVLTGQVPTFMIGSDAFQEADTV
CHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHCCC
GITRPCTKHNWLVKETERLSGVIHEAFHVATSGRPGPVLVDIPKDVQFATGTYTPPKPST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCCCCCCC
SHYQPKLKGDMEAITELVAALEKAKKPIFYTGGGVINSGPAASQLLRELVQATGFPITST
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPASGENWIGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRLDAFSPKSIK
HHHCCCCCCCCCCCEEECCCCCHHHCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCEE
AHIDIDPSSINKVIKTDIPVVGDIAHVLEDVLKVWKSRGRKTNSEAVAKWWAQIEQWKTV
EEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
DCLRFTQEGKVIKPQHALARLEALTKKYDRYICTEVGQHQMWAAQYLGFEDPNRWMTSGG
HHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCC
LGTMGYGFPASIGVQIAHPDALVINVAGEASWLMNMQEMGTAVQYRLPVKQFILNNERLG
CCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHCCEEEEECCHHHHHHCCCCCH
MVRQWQELLHGERYSHSWSDALPDFVKLAEAFGAKGILCSDPDDLDDAIMEMLTHDGPVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEE
FDCLVTKHENCFPMIPSGKAHNEMLLGEADTQGVIDAKGSVLV
EEEEHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCC
>Mature Secondary Structure 
TRQMSGAKMIVQALIDQGVDTVFGYPGGAVLPIYDEVFQQNAIRHILVRHEQGAVHAAE
CCCCCHHHHHHHHHHHCCCHHEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCC
GYARATGKPGVVLVTSGPGATNAVTGLTDALMDSIPLVVLTGQVPTFMIGSDAFQEADTV
CHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHCCC
GITRPCTKHNWLVKETERLSGVIHEAFHVATSGRPGPVLVDIPKDVQFATGTYTPPKPST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCCCCCCC
SHYQPKLKGDMEAITELVAALEKAKKPIFYTGGGVINSGPAASQLLRELVQATGFPITST
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPASGENWIGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRLDAFSPKSIK
HHHCCCCCCCCCCCEEECCCCCHHHCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCEE
AHIDIDPSSINKVIKTDIPVVGDIAHVLEDVLKVWKSRGRKTNSEAVAKWWAQIEQWKTV
EEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
DCLRFTQEGKVIKPQHALARLEALTKKYDRYICTEVGQHQMWAAQYLGFEDPNRWMTSGG
HHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCC
LGTMGYGFPASIGVQIAHPDALVINVAGEASWLMNMQEMGTAVQYRLPVKQFILNNERLG
CCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHCCEEEEECCHHHHHHCCCCCH
MVRQWQELLHGERYSHSWSDALPDFVKLAEAFGAKGILCSDPDDLDDAIMEMLTHDGPVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEE
FDCLVTKHENCFPMIPSGKAHNEMLLGEADTQGVIDAKGSVLV
EEEEHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]