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The map label for this gene is ntrC [H]

Identifier: 110679973

GI number: 110679973

Start: 2636234

End: 2637598

Strand: Reverse

Name: ntrC [H]

Synonym: RD1_2763

Alternate gene names: 110679973

Gene position: 2637598-2636234 (Counterclockwise)

Preceding gene: 110679974

Following gene: 110679972

Centisome position: 63.82

GC content: 60.15

Gene sequence:

>1365_bases
ATGGACGGGACAATACTTGTAGCAGATGATGATCGGACAATTCGCACCGTCCTGACGCAAGCGCTGACGCGGGCCGGATG
CAAGGTACATGCGACCTCCAGCCTGACCACGTTGCTGCGATGGGTGGGCGAGGGCAAAGGCGATGTGGTGATCTCTGATG
TGATGATGCCCGATGGGAATGGTCTTGAGATGTTGCCAAAGATTGCACAGGACCGGCCCGGCCTGCCGGTGATCGTGATC
TCTGCACAAAATACCATCATGACGGCCATCAAGGCGGCTGAGGCCGAGGCCTATGATTACCTGCCCAAGCCCTTTGATCT
GCCTGATCTGATGAAGCGGACGGCCCGTGCCCTTGAAATGCAGCAGCAGAACCGCCGCGCGCCCGAGATCAGCGATGATC
GCCCCGATGATCTCCCGCTCGTGGGGCGCACCGCCGTGATGCAATCGCTTTACCGGGTCGTTGCGCGGGTGATGAACGCG
GATCTTCCGGTATTGATCTGGGGGGAATCCGGCACGGGCAAATCATTGATATCGCGGGCAATACATGACTTCTCCGACAG
GCGCAGCTTGCCGTTTGTCTCTGCCACGGCGGCGGATTTGCAGGAACTTGAGGGCCCCGCACGTCTTTTGGCGCGGGTCA
AGGGCGGCACCTTGTTGGTGGAGGAAATCGCCGATCTGCCAGAAGAGTTGCAGGCGCGGCTGGTGCGCATGATGGATTCG
CCGGGCGAATACGCCCCGCGCTTTCTGGCCACCAGCCAGACGGACCTCGCCGGTGCCATGGAGGCGGGCAATGTGCGCAA
AGACCTTTATTATCGCCTGAGTGGCGCAACCCTGACAGTGCCATCCCTGCGCGAACGGGTTGATGATATTCCGCTGTTGG
CAGAGCATTTTCTGACCCGCGCCGAAAGCTCCGGTGCGGCACCGCGCAGCCTGTCGGATGCGGCGGCCGAGATTTTCCGA
AACTACAGTTGGCCCGGCAATGTGCGGCAGTTGGAAAACGCCATCAGACGGCTCGGCCTGACCAGCCGCGCGGCGGAAAT
CACCGCGTCCGAGGTTGAGCAGGTGCTGGGGGCGCAGCCTGACCTCGAACCGATGCGCGCGGCGGGCAATACCGAAAAAC
TGGGGGCCTCTGTCGAACGCCACTTGCGGCGGTATTTCGATTTGCATGGCAATATGTTGCCGCCGTCGGGCCTTTATGCG
CGCATCCTGCGCGAGGTCGAAGCGCCGCTGATCGAAATCGCACTGGAGGCCACGGGCGGGAATCAGGCCAAATGTGCTGA
TTTACTTGGGATAAATCGCAATACGCTCAGAAAAAAGATCACCGATCTGGATATAGAGGTGACACGGCGTCGCAAATTGA
TGTAA

Upstream 100 bases:

>100_bases
GATTTCCGTGACATCGGTTCCCGGCCAGACTGTATTTCGCATCTCTCTGGCGCGCGCCCCGAAAGATGCCGTTGCCAAGG
ACACATAGGAGGCTCAGGCC

Downstream 100 bases:

>100_bases
AACTGCCACACAAACGTGGCCATCAGGGGACACCCCTTGTTCAGGACCACAAAATAGAGCGGTTCGCGCCCATGGCGCAC
CATCATATGCGAGGATCTTG

Product: nitrogen regulation protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MDGTILVADDDRTIRTVLTQALTRAGCKVHATSSLTTLLRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPDLMKRTARALEMQQQNRRAPEISDDRPDDLPLVGRTAVMQSLYRVVARVMNA
DLPVLIWGESGTGKSLISRAIHDFSDRRSLPFVSATAADLQELEGPARLLARVKGGTLLVEEIADLPEELQARLVRMMDS
PGEYAPRFLATSQTDLAGAMEAGNVRKDLYYRLSGATLTVPSLRERVDDIPLLAEHFLTRAESSGAAPRSLSDAAAEIFR
NYSWPGNVRQLENAIRRLGLTSRAAEITASEVEQVLGAQPDLEPMRAAGNTEKLGASVERHLRRYFDLHGNMLPPSGLYA
RILREVEAPLIEIALEATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM

Sequences:

>Translated_454_residues
MDGTILVADDDRTIRTVLTQALTRAGCKVHATSSLTTLLRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPDLMKRTARALEMQQQNRRAPEISDDRPDDLPLVGRTAVMQSLYRVVARVMNA
DLPVLIWGESGTGKSLISRAIHDFSDRRSLPFVSATAADLQELEGPARLLARVKGGTLLVEEIADLPEELQARLVRMMDS
PGEYAPRFLATSQTDLAGAMEAGNVRKDLYYRLSGATLTVPSLRERVDDIPLLAEHFLTRAESSGAAPRSLSDAAAEIFR
NYSWPGNVRQLENAIRRLGLTSRAAEITASEVEQVLGAQPDLEPMRAAGNTEKLGASVERHLRRYFDLHGNMLPPSGLYA
RILREVEAPLIEIALEATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
>Mature_454_residues
MDGTILVADDDRTIRTVLTQALTRAGCKVHATSSLTTLLRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPDLMKRTARALEMQQQNRRAPEISDDRPDDLPLVGRTAVMQSLYRVVARVMNA
DLPVLIWGESGTGKSLISRAIHDFSDRRSLPFVSATAADLQELEGPARLLARVKGGTLLVEEIADLPEELQARLVRMMDS
PGEYAPRFLATSQTDLAGAMEAGNVRKDLYYRLSGATLTVPSLRERVDDIPLLAEHFLTRAESSGAAPRSLSDAAAEIFR
NYSWPGNVRQLENAIRRLGLTSRAAEITASEVEQVLGAQPDLEPMRAAGNTEKLGASVERHLRRYFDLHGNMLPPSGLYA
RILREVEAPLIEIALEATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=476, Percent_Identity=32.3529411764706, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1790437, Length=468, Percent_Identity=33.7606837606838, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=30.398322851153, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=32.1888412017167, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789233, Length=345, Percent_Identity=31.5942028985507, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082152, Length=249, Percent_Identity=36.9477911646586, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87082117, Length=265, Percent_Identity=33.9622641509434, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786524, Length=346, Percent_Identity=30.3468208092486, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789087, Length=237, Percent_Identity=34.5991561181435, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787583, Length=197, Percent_Identity=33.502538071066, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081858, Length=318, Percent_Identity=28.3018867924528, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789828, Length=255, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=35.6521739130435, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786784, Length=110, Percent_Identity=30.9090909090909, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=30, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49865; Mature: 49865

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTILVADDDRTIRTVLTQALTRAGCKVHATSSLTTLLRWVGEGKGDVVISDVMMPDGN
CCCEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLEMLPKIAQDRPGLPVIVISAQNTIMTAIKAAEAEAYDYLPKPFDLPDLMKRTARALEM
CHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QQQNRRAPEISDDRPDDLPLVGRTAVMQSLYRVVARVMNADLPVLIWGESGTGKSLISRA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
IHDFSDRRSLPFVSATAADLQELEGPARLLARVKGGTLLVEEIADLPEELQARLVRMMDS
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHHHCCHHHHHHHHHHHHCC
PGEYAPRFLATSQTDLAGAMEAGNVRKDLYYRLSGATLTVPSLRERVDDIPLLAEHFLTR
CCCCCCHHHCCCCHHHHHHHHHCCHHHHHHEEECCCEEECHHHHHHHCCCHHHHHHHHHH
AESSGAAPRSLSDAAAEIFRNYSWPGNVRQLENAIRRLGLTSRAAEITASEVEQVLGAQP
HHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DLEPMRAAGNTEKLGASVERHLRRYFDLHGNMLPPSGLYARILREVEAPLIEIALEATGG
CCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
NQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
CCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MDGTILVADDDRTIRTVLTQALTRAGCKVHATSSLTTLLRWVGEGKGDVVISDVMMPDGN
CCCEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLEMLPKIAQDRPGLPVIVISAQNTIMTAIKAAEAEAYDYLPKPFDLPDLMKRTARALEM
CHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QQQNRRAPEISDDRPDDLPLVGRTAVMQSLYRVVARVMNADLPVLIWGESGTGKSLISRA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
IHDFSDRRSLPFVSATAADLQELEGPARLLARVKGGTLLVEEIADLPEELQARLVRMMDS
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHHHCCHHHHHHHHHHHHCC
PGEYAPRFLATSQTDLAGAMEAGNVRKDLYYRLSGATLTVPSLRERVDDIPLLAEHFLTR
CCCCCCHHHCCCCHHHHHHHHHCCHHHHHHEEECCCEEECHHHHHHHCCCHHHHHHHHHH
AESSGAAPRSLSDAAAEIFRNYSWPGNVRQLENAIRRLGLTSRAAEITASEVEQVLGAQP
HHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DLEPMRAAGNTEKLGASVERHLRRYFDLHGNMLPPSGLYARILREVEAPLIEIALEATGG
CCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
NQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
CCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]