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The map label for this gene is ntrB [H]

Identifier: 110679974

GI number: 110679974

Start: 2637611

End: 2638690

Strand: Reverse

Name: ntrB [H]

Synonym: RD1_2764

Alternate gene names: 110679974

Gene position: 2638690-2637611 (Counterclockwise)

Preceding gene: 110679975

Following gene: 110679973

Centisome position: 63.84

GC content: 58.24

Gene sequence:

>1080_bases
ATGATGCAGGACAATTCCCTATGGGCAAGCCTGCCGGTGCCGACGCTGATCGTCGGGCCACAAAACAGCATCGTCGATGT
GAATTCCGCCGCCGAAGGGTTTCTGAACGTATCCGCAAAATCCTTGCGTGACACGCCCTTGTGGGACCAGATTGCGGTGA
ATGACCCCTTGGAGGCGGCGTTTGAACGGGCCCGTGAAACCAGCACGCCGTTGTTCGTCAATGATGTTGATGTGGGCGGG
GGCGCGCGCGCGCCGATGCAATCCGATCTGCAGGTCGCACCCCTGTTGGGGCGTCCCGGCCATATGATCCTGATGATCTC
GCCGCGCGAACTGGCGGGTCGGATGACGCAGAACTATTCGGTGAAATCGGCTGCAAAATCCGCCATCGGCATGGCTGAAA
TGCTTGCCCACGAGATCAAGAACCCGCTGGCCGGTATCACGGGTGCGGCACAGTTACTCAGCATGAACTTGAGCCCGGAA
GACCTTGAATTAACAGACTTGATCGTCGAAGAAAGCCGGCGCATCGTCAAGCTGTTGGAGCAGGTTGAGCAATTTGGCAA
TCTGTCCGTGCCGGATTTCAAGCCGGTGAACATTCACGATGTTCTGGATCGTGCGCGTCGGTCTGCGCTGCTGGGGTTCG
GGGCGCATATGAACATCATAGAAGAATACGATCCATCCCTGCCGCTGGCCTATGGGGATCCGGATCAATTGCTGCAGGTT
GTTCTGAACCTGCTTAAAAACGCGTCTGAAGCGGCGGGCAGTGCGCCGGGTAAAATCCGGCTGCGCAGCTATTTCGAACA
CTCCTTTCGGCTCAGGCGCTCTGATGGCTCCGGGCGGTCGCTTCCTCTGCAGATCGAGGTGATCGACGATGGTCCGGGCC
TGCCGGAAACCATCAAGGGCGACATTTTCGACCCATTCGTATCGGGCAAGGAAAACGGCACGGGTCTCGGTCTCGCGCTG
GTCAGCAAGATCATCTCGGACCACCATGGCTGGATTTCCGTGACATCGGTTCCCGGCCAGACTGTATTTCGCATCTCTCT
GGCGCGCGCCCCGAAAGATGCCGTTGCCAAGGACACATAG

Upstream 100 bases:

>100_bases
CGCCCAAGGACATCCGCAAGGCTGTGCTGACCGCGCAAGATGCGGTGACGGTGGACGGGCTGCTGGCGCAAGCCTGTGCC
TGGCCGGATCAGGTCGCCGC

Downstream 100 bases:

>100_bases
GAGGCTCAGGCCATGGACGGGACAATACTTGTAGCAGATGATGATCGGACAATTCGCACCGTCCTGACGCAAGCGCTGAC
GCGGGCCGGATGCAAGGTAC

Product: nitrogen regulation protein NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MMQDNSLWASLPVPTLIVGPQNSIVDVNSAAEGFLNVSAKSLRDTPLWDQIAVNDPLEAAFERARETSTPLFVNDVDVGG
GARAPMQSDLQVAPLLGRPGHMILMISPRELAGRMTQNYSVKSAAKSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSPE
DLELTDLIVEESRRIVKLLEQVEQFGNLSVPDFKPVNIHDVLDRARRSALLGFGAHMNIIEEYDPSLPLAYGDPDQLLQV
VLNLLKNASEAAGSAPGKIRLRSYFEHSFRLRRSDGSGRSLPLQIEVIDDGPGLPETIKGDIFDPFVSGKENGTGLGLAL
VSKIISDHHGWISVTSVPGQTVFRISLARAPKDAVAKDT

Sequences:

>Translated_359_residues
MMQDNSLWASLPVPTLIVGPQNSIVDVNSAAEGFLNVSAKSLRDTPLWDQIAVNDPLEAAFERARETSTPLFVNDVDVGG
GARAPMQSDLQVAPLLGRPGHMILMISPRELAGRMTQNYSVKSAAKSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSPE
DLELTDLIVEESRRIVKLLEQVEQFGNLSVPDFKPVNIHDVLDRARRSALLGFGAHMNIIEEYDPSLPLAYGDPDQLLQV
VLNLLKNASEAAGSAPGKIRLRSYFEHSFRLRRSDGSGRSLPLQIEVIDDGPGLPETIKGDIFDPFVSGKENGTGLGLAL
VSKIISDHHGWISVTSVPGQTVFRISLARAPKDAVAKDT
>Mature_359_residues
MMQDNSLWASLPVPTLIVGPQNSIVDVNSAAEGFLNVSAKSLRDTPLWDQIAVNDPLEAAFERARETSTPLFVNDVDVGG
GARAPMQSDLQVAPLLGRPGHMILMISPRELAGRMTQNYSVKSAAKSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSPE
DLELTDLIVEESRRIVKLLEQVEQFGNLSVPDFKPVNIHDVLDRARRSALLGFGAHMNIIEEYDPSLPLAYGDPDQLLQV
VLNLLKNASEAAGSAPGKIRLRSYFEHSFRLRRSDGSGRSLPLQIEVIDDGPGLPETIKGDIFDPFVSGKENGTGLGLAL
VSKIISDHHGWISVTSVPGQTVFRISLARAPKDAVAKDT

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=346, Percent_Identity=33.2369942196532, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=28.8372093023256, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081816, Length=237, Percent_Identity=32.0675105485232, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=227, Percent_Identity=28.1938325991189, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38752; Mature: 38752

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQDNSLWASLPVPTLIVGPQNSIVDVNSAAEGFLNVSAKSLRDTPLWDQIAVNDPLEAA
CCCCCCEEEECCCCEEEECCCCCEEECCCHHHHHHCCCHHHHCCCCCCCHHCCCCHHHHH
FERARETSTPLFVNDVDVGGGARAPMQSDLQVAPLLGRPGHMILMISPRELAGRMTQNYS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCCC
VKSAAKSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSPEDLELTDLIVEESRRIVKLLE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
QVEQFGNLSVPDFKPVNIHDVLDRARRSALLGFGAHMNIIEEYDPSLPLAYGDPDQLLQV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHH
VLNLLKNASEAAGSAPGKIRLRSYFEHSFRLRRSDGSGRSLPLQIEVIDDGPGLPETIKG
HHHHHHCHHHHCCCCCCEEHHHHHHHHHHEEEECCCCCCCCEEEEEEECCCCCCCHHCCC
DIFDPFVSGKENGTGLGLALVSKIISDHHGWISVTSVPGQTVFRISLARAPKDAVAKDT
CCCCHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCHHCCCCC
>Mature Secondary Structure
MMQDNSLWASLPVPTLIVGPQNSIVDVNSAAEGFLNVSAKSLRDTPLWDQIAVNDPLEAA
CCCCCCEEEECCCCEEEECCCCCEEECCCHHHHHHCCCHHHHCCCCCCCHHCCCCHHHHH
FERARETSTPLFVNDVDVGGGARAPMQSDLQVAPLLGRPGHMILMISPRELAGRMTQNYS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCCC
VKSAAKSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSPEDLELTDLIVEESRRIVKLLE
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
QVEQFGNLSVPDFKPVNIHDVLDRARRSALLGFGAHMNIIEEYDPSLPLAYGDPDQLLQV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHH
VLNLLKNASEAAGSAPGKIRLRSYFEHSFRLRRSDGSGRSLPLQIEVIDDGPGLPETIKG
HHHHHHCHHHHCCCCCCEEHHHHHHHHHHEEEECCCCCCCCEEEEEEECCCCCCCHHCCC
DIFDPFVSGKENGTGLGLALVSKIISDHHGWISVTSVPGQTVFRISLARAPKDAVAKDT
CCCCHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]