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The map label for this gene is ntrX [H]

Identifier: 110679971

GI number: 110679971

Start: 2632469

End: 2633881

Strand: Reverse

Name: ntrX [H]

Synonym: RD1_2761

Alternate gene names: 110679971

Gene position: 2633881-2632469 (Counterclockwise)

Preceding gene: 110679972

Following gene: 110679968

Centisome position: 63.73

GC content: 58.17

Gene sequence:

>1413_bases
ATGAGTGATATTCTGATTGTTGATGATGAACGCGATATTCGCGAACTGATCTCGGATATTCTGATTGACGAAGGCTATGC
CACCCGGCTCGCCGGAAATTCGGACGATGCGATGTCGGCCATCAACGCGGAAGCGCCTGCGCTGATCATCCTCGATATCT
GGCTGAAAGACAGCCGCATGGATGGGATTGATATTCTCAAGGCGGTCAAACGCGACAATCCCGATGTGCCGGTGGTGATC
ATTTCCGGGCATGGCAATATCGAAATCGCGGTCGCAGCCATCAAACAGGGGGCGTATGATTTCATCGAAAAACCCTTTAA
CATCGACCAGTTACTCGTGGTGATCCGCCGGGCGATGGAGACATCGCGTCTGCGCCAGGAAAACCAGAGCCTGAAACGCC
GGGACGTGTCAGCTGCGGATATGATCGGTTCGTCTGCGGCCTTCCGCACCATGGTGAGCCAGCTGGACAAGGTCACGAAA
TCCAATGGACGCGTGATGCTGACGGGGCCGGCAGGCTGCGGCAAGGAACTGGCAGCGCGGTATATTCACACCAATTCCAA
CCGTGCCTCCGCACCCTTTGTGACGGTGAACTGCGCCGGTGTTGCGCCGGATCGTATGGAAGAGGTGCTGTTCGGGCGGG
AATCATCTGAACGTGGTATCGAACCGGGCCTGCTGGAAGAGGCGCATGGTGGCATCATCTATTTTGATGAAGTGGCCGAT
ATGCCCGAGGGCACGCAGTCGAAGATCCTGCGGGTGCTGGTTGACCAGCAATTCCAGCGGGTCGGGGGCAATGACACGGT
GCGCGTCGATTTGCGGGTGATCTCATCGACCAGTCGCAATCTTGAACAGGCGATTGCTGCAGGGACCTTCCGTCAGGAAC
TCTATCACCGGCTGAATGTGGTGCCGATCGCGGTGCCATCGCTGGAAGACCGCCGCGAGGATATCCCGGAGTTGGCAGCG
GCTTTCATCGCGGAAATGAATGCGTCCCAAGGTCTGGCCTTGCGTGAACTCAGCGAGGATGCCATCGCGCTGATGCAAAC
GATGCAATGGCCGGGCAACGTGCGCCAGTTGAAAAACCTGATCGAACGGGTGCTGATCCTCGGCGACAGCAACGGGCCGA
TTGAAGCGCGCGAGTTGCCGGGCATCGAGGAAGGCGGCAATAGCGACAGCGGGATCGTCCTGTCAGGTGCCCTCGCTACG
CTTGCCCTGCGCGAAGCGCGTGAAGCATTCGAGCGGGAATACCTGCTGACGCAGATCAACCGCTTTGGCGGCAACATCTC
GAAAACGGCGAATTTCGTCGGGATGGAACGCAGCGCGCTACACCGTAAACTCAAATCGCTCGGCGTTGTGACATCGGCCA
AATCCGGCACGCGGGTTGCGCATATTGATGAGGATGCCGAGGCGGTGGGCTAA

Upstream 100 bases:

>100_bases
TTTGGCACGGATACCCATGTTGGCGCGATGGCGGTGATCATGCTGCCCATCGTTGCCTCGCAACCCGAAAAAACACTCCC
GCAAGAAGGACTCCTGACCC

Downstream 100 bases:

>100_bases
AGCGTCATGCGAAAAACCTGAAGTACGCAACGCTGCCTGAAATCAATGATTTCAACGCGTTACGTGATGTTTCATGTGTT
TCGTCAGACGCTATGGACGT

Product: nitrogen assimilation regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDILIVDDERDIRELISDILIDEGYATRLAGNSDDAMSAINAEAPALIILDIWLKDSRMDGIDILKAVKRDNPDVPVVI
ISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLLVVIRRAMETSRLRQENQSLKRRDVSAADMIGSSAAFRTMVSQLDKVTK
SNGRVMLTGPAGCGKELAARYIHTNSNRASAPFVTVNCAGVAPDRMEEVLFGRESSERGIEPGLLEEAHGGIIYFDEVAD
MPEGTQSKILRVLVDQQFQRVGGNDTVRVDLRVISSTSRNLEQAIAAGTFRQELYHRLNVVPIAVPSLEDRREDIPELAA
AFIAEMNASQGLALRELSEDAIALMQTMQWPGNVRQLKNLIERVLILGDSNGPIEARELPGIEEGGNSDSGIVLSGALAT
LALREAREAFEREYLLTQINRFGGNISKTANFVGMERSALHRKLKSLGVVTSAKSGTRVAHIDEDAEAVG

Sequences:

>Translated_470_residues
MSDILIVDDERDIRELISDILIDEGYATRLAGNSDDAMSAINAEAPALIILDIWLKDSRMDGIDILKAVKRDNPDVPVVI
ISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLLVVIRRAMETSRLRQENQSLKRRDVSAADMIGSSAAFRTMVSQLDKVTK
SNGRVMLTGPAGCGKELAARYIHTNSNRASAPFVTVNCAGVAPDRMEEVLFGRESSERGIEPGLLEEAHGGIIYFDEVAD
MPEGTQSKILRVLVDQQFQRVGGNDTVRVDLRVISSTSRNLEQAIAAGTFRQELYHRLNVVPIAVPSLEDRREDIPELAA
AFIAEMNASQGLALRELSEDAIALMQTMQWPGNVRQLKNLIERVLILGDSNGPIEARELPGIEEGGNSDSGIVLSGALAT
LALREAREAFEREYLLTQINRFGGNISKTANFVGMERSALHRKLKSLGVVTSAKSGTRVAHIDEDAEAVG
>Mature_469_residues
SDILIVDDERDIRELISDILIDEGYATRLAGNSDDAMSAINAEAPALIILDIWLKDSRMDGIDILKAVKRDNPDVPVVII
SGHGNIEIAVAAIKQGAYDFIEKPFNIDQLLVVIRRAMETSRLRQENQSLKRRDVSAADMIGSSAAFRTMVSQLDKVTKS
NGRVMLTGPAGCGKELAARYIHTNSNRASAPFVTVNCAGVAPDRMEEVLFGRESSERGIEPGLLEEAHGGIIYFDEVADM
PEGTQSKILRVLVDQQFQRVGGNDTVRVDLRVISSTSRNLEQAIAAGTFRQELYHRLNVVPIAVPSLEDRREDIPELAAA
FIAEMNASQGLALRELSEDAIALMQTMQWPGNVRQLKNLIERVLILGDSNGPIEARELPGIEEGGNSDSGIVLSGALATL
ALREAREAFEREYLLTQINRFGGNISKTANFVGMERSALHRKLKSLGVVTSAKSGTRVAHIDEDAEAVG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=33.0396475770925, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=34.475374732334, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=34.8017621145374, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788905, Length=455, Percent_Identity=34.0659340659341, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=33.6257309941521, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789087, Length=351, Percent_Identity=34.1880341880342, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787583, Length=342, Percent_Identity=34.2105263157895, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=33.6391437308869, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=30.9597523219814, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=34.0490797546012, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=29.746835443038, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=28.5234899328859, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789828, Length=269, Percent_Identity=31.9702602230483, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=35.5932203389831, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=178, Percent_Identity=29.2134831460674, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=126, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51297; Mature: 51166

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILIVDDERDIRELISDILIDEGYATRLAGNSDDAMSAINAEAPALIILDIWLKDSRM
CCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCEEEEEEEEECCCCC
DGIDILKAVKRDNPDVPVVIISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLLVVIRRAME
CHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRQENQSLKRRDVSAADMIGSSAAFRTMVSQLDKVTKSNGRVMLTGPAGCGKELAAR
HHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
YIHTNSNRASAPFVTVNCAGVAPDRMEEVLFGRESSERGIEPGLLEEAHGGIIYFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCC
MPEGTQSKILRVLVDQQFQRVGGNDTVRVDLRVISSTSRNLEQAIAAGTFRQELYHRLNV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCE
VPIAVPSLEDRREDIPELAAAFIAEMNASQGLALRELSEDAIALMQTMQWPGNVRQLKNL
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IERVLILGDSNGPIEARELPGIEEGGNSDSGIVLSGALATLALREAREAFEREYLLTQIN
HHHHEEECCCCCCCCHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RFGGNISKTANFVGMERSALHRKLKSLGVVTSAKSGTRVAHIDEDAEAVG
HHCCCHHHHHHHHCCHHHHHHHHHHHHCCEEECCCCCEEEEECCHHHHCC
>Mature Secondary Structure 
SDILIVDDERDIRELISDILIDEGYATRLAGNSDDAMSAINAEAPALIILDIWLKDSRM
CCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCEEEEEEEEECCCCC
DGIDILKAVKRDNPDVPVVIISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLLVVIRRAME
CHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRQENQSLKRRDVSAADMIGSSAAFRTMVSQLDKVTKSNGRVMLTGPAGCGKELAAR
HHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
YIHTNSNRASAPFVTVNCAGVAPDRMEEVLFGRESSERGIEPGLLEEAHGGIIYFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCC
MPEGTQSKILRVLVDQQFQRVGGNDTVRVDLRVISSTSRNLEQAIAAGTFRQELYHRLNV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCE
VPIAVPSLEDRREDIPELAAAFIAEMNASQGLALRELSEDAIALMQTMQWPGNVRQLKNL
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IERVLILGDSNGPIEARELPGIEEGGNSDSGIVLSGALATLALREAREAFEREYLLTQIN
HHHHEEECCCCCCCCHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RFGGNISKTANFVGMERSALHRKLKSLGVVTSAKSGTRVAHIDEDAEAVG
HHCCCHHHHHHHHCCHHHHHHHHHHHHCCEEECCCCCEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]