Click here to switch to the map view.

The map label for this gene is rrm2 [H]

Identifier: 110679943

GI number: 110679943

Start: 2601816

End: 2602571

Strand: Reverse

Name: rrm2 [H]

Synonym: RD1_2729

Alternate gene names: 110679943

Gene position: 2602571-2601816 (Counterclockwise)

Preceding gene: 110679945

Following gene: 110679942

Centisome position: 62.97

GC content: 62.17

Gene sequence:

>756_bases
ATGACAGAAACAATCACCACCCCAACCACCCCGGCCTTTGTTCTGGTCCGTCCGCAGATGGGCGAAAACATCGGTGCCTC
CGCCCGTGCGATGTGGAATTTCGGGCTTGAGCATATGCGTGTGGTGGCCCCGCGCGATGGTTGGCCAAATCAGGCGGCGG
TTGCGCTGGCCAGCGGCGCGGGCCGGGTTCTGGACAATGCGCAGTTGAGCGATGATTTGCCCGGTAGTGTGGGCGATTGT
TCCTTTGTCTTTGCGACGACAGCGCGCCCGCGCGACCTGACCAAACCTGTGTTCAGCCCCGAAGAGGCGATGCGCGTGGC
AGCGCAGCGGATCGCGGCGGGCGAGCGCATTGCGGTGCTTTTCGGGCCGGAGCGGGCAGGGCTGGAAAACGATGACATTG
CGCGCGCCAATGCGATCATATCGGTGCCTGTCAACCCGGCCTTCCCCTCGCTGAACCTTGCGCAATGCGTGTTGCTGGTG
GGCTATGAATGGATGCGGGCCACGCACGATGTGACGCCGCACACGGTCGATATGTCGGGAACGCAATGGGCTGATCATAT
CGAGGTTGAACACCTCGCCACCCATTACGAGGACCGGCTGGAAGAGATGGGGTTCTTTTTCCCGGCGCATAAATCGCACT
CCATGCGGGTCAATCTGCGCAACTTCTGGAGCCGGATGCCCATGACGCGCGCGGATGTTCAGATGCTGCACGGGATGATG
CGGCAGATGGTCCGCTGGAAGACGCGCGGCGACTAG

Upstream 100 bases:

>100_bases
CCATGGGGAATGAAAACGCATTTGACCCCGGCGCGTCAAAATTGCCGCCCGACTGTTGCGGCCCGTTGCGCCGCCGCGCC
GCGCGACGTAAGACGCAGGC

Downstream 100 bases:

>100_bases
TCGCGCTGGACCTCTCCAGCGCATCCCCCTAGGGTCGCGGCGCGACAGTTTGAGGATATCCAATGAGCAGCAAACGCAAG
TTGTTTGAAGAAGTTGGGGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTETITTPTTPAFVLVRPQMGENIGASARAMWNFGLEHMRVVAPRDGWPNQAAVALASGAGRVLDNAQLSDDLPGSVGDC
SFVFATTARPRDLTKPVFSPEEAMRVAAQRIAAGERIAVLFGPERAGLENDDIARANAIISVPVNPAFPSLNLAQCVLLV
GYEWMRATHDVTPHTVDMSGTQWADHIEVEHLATHYEDRLEEMGFFFPAHKSHSMRVNLRNFWSRMPMTRADVQMLHGMM
RQMVRWKTRGD

Sequences:

>Translated_251_residues
MTETITTPTTPAFVLVRPQMGENIGASARAMWNFGLEHMRVVAPRDGWPNQAAVALASGAGRVLDNAQLSDDLPGSVGDC
SFVFATTARPRDLTKPVFSPEEAMRVAAQRIAAGERIAVLFGPERAGLENDDIARANAIISVPVNPAFPSLNLAQCVLLV
GYEWMRATHDVTPHTVDMSGTQWADHIEVEHLATHYEDRLEEMGFFFPAHKSHSMRVNLRNFWSRMPMTRADVQMLHGMM
RQMVRWKTRGD
>Mature_250_residues
TETITTPTTPAFVLVRPQMGENIGASARAMWNFGLEHMRVVAPRDGWPNQAAVALASGAGRVLDNAQLSDDLPGSVGDCS
FVFATTARPRDLTKPVFSPEEAMRVAAQRIAAGERIAVLFGPERAGLENDDIARANAIISVPVNPAFPSLNLAQCVLLVG
YEWMRATHDVTPHTVDMSGTQWADHIEVEHLATHYEDRLEEMGFFFPAHKSHSMRVNLRNFWSRMPMTRADVQMLHGMMR
QMVRWKTRGD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=233, Percent_Identity=32.1888412017167, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790865, Length=157, Percent_Identity=33.7579617834395, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27911; Mature: 27780

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETITTPTTPAFVLVRPQMGENIGASARAMWNFGLEHMRVVAPRDGWPNQAAVALASGA
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCHHHEEEEECCCCCCCHHEEEEECCC
GRVLDNAQLSDDLPGSVGDCSFVFATTARPRDLTKPVFSPEEAMRVAAQRIAAGERIAVL
CCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
FGPERAGLENDDIARANAIISVPVNPAFPSLNLAQCVLLVGYEWMRATHDVTPHTVDMSG
ECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCC
TQWADHIEVEHLATHYEDRLEEMGFFFPAHKSHSMRVNLRNFWSRMPMTRADVQMLHGMM
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHH
RQMVRWKTRGD
HHHHHHHCCCC
>Mature Secondary Structure 
TETITTPTTPAFVLVRPQMGENIGASARAMWNFGLEHMRVVAPRDGWPNQAAVALASGA
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCHHHEEEEECCCCCCCHHEEEEECCC
GRVLDNAQLSDDLPGSVGDCSFVFATTARPRDLTKPVFSPEEAMRVAAQRIAAGERIAVL
CCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
FGPERAGLENDDIARANAIISVPVNPAFPSLNLAQCVLLVGYEWMRATHDVTPHTVDMSG
ECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCC
TQWADHIEVEHLATHYEDRLEEMGFFFPAHKSHSMRVNLRNFWSRMPMTRADVQMLHGMM
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHH
RQMVRWKTRGD
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]