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The map label for this gene is proB

Identifier: 110679885

GI number: 110679885

Start: 2537759

End: 2538883

Strand: Reverse

Name: proB

Synonym: RD1_2659

Alternate gene names: 110679885

Gene position: 2538883-2537759 (Counterclockwise)

Preceding gene: 110679886

Following gene: 110679884

Centisome position: 61.43

GC content: 62.67

Gene sequence:

>1125_bases
ATGGCGGCCCTGACGGACGCAGATTGTCTGGTCGTCAAGATCGGGTCCGCCCTGTTGGTGGACCGGGACGCGGGCGGCTT
GCGGCAGGCGTGGCTTGCGTCGCTGGCGCAGGATGTGGCTTGGTTGACGCGGCAGGGAGTAAAGGTCGTGCTCGTGTCCT
CCGGCTCTATCGCGCTAGGGCGCGGCATCCTGAAGCTCTCCGACACCGATTTGCCGTTGGAACAGGCGCAGGCGGCCGCG
GCGGTGGGTCAAATCCGCCTTGCGCGCGCCTATGAAGAAGTCCTCGCGCCGCATGGGATCACCACGGGACAGGTTCTGAT
GACGCTGGATGACAGCTCGAACCGGCGGCGATATCTCAACAGCCGCGCGACGCTGAAACAACTGCTGAGCATGGGCGTCG
TGCCCATCGTGAACGAAAACGACACCATTGCCACAGATGAAATCCGCTTTGGGGATAACGACCGGCTGGCGGCGCAAATC
GCGGTAACGGTCGGGGCGGATCAGCTTGTCTTGCTCTCGGATGTGGATGGGTTCTATTCGGCCAACCCCAACATCGACCC
CACCGCGCAACGCTTTGATGAAATTGGTGAAATCACGCCTGAAATCGAGGACATGGCGGGCGACGCCTGTTCCGGCCTGT
CCAAGGGCGGCATGAAAACCAAACTGATGGCGGCCAAGATTGCAACCGCGGCCGGATGCCGGATGGCGATTACCGAAGGC
GTCGTTCTCAATCCGCTGCGGGCATTGAATGAGGGTGCTGCGGCCACATGGTTTCGGGCCGATACAGACCCGCAGGCGGC
GCGCAAGGGGTGGATTGCCGCCATGAAACCCAAAGGCACTGTGCATCTGGACAGCGGGGCGCTGGCTGCTTTGCGGTCGG
GCAAGAGCCTGCTGCCTGCCGGGGTGTCGCACATCACGGGCCATTTTCAGCGCGGCGACCCGGTGGCGATCGCCGATGGG
GCCGGGCATACCGTGGGTCACGGTCTTGTGCGCTACACGGCGGAGGAAGCGGGCAGGATCATGGGGCGCAAGTCCAATGA
GATTGAATCGGTCCTTGGCTATCCCGCGCGTGCGGCTTTGATTCACCGGGACGATATGGCATTAACGATCCAGAATGGCG
ATTGA

Upstream 100 bases:

>100_bases
CCGGTGTTGCCGGGGACGGCGTCACTGATGTGTTGCGAAAACTGCGCGGCGAGATCAGCGATGAGCCGCTGCGCCATAAA
CCTGTCGAGGAAAAAGAACC

Downstream 100 bases:

>100_bases
TTTCGAAGAGGGCGTGAGGGGGTAAGCAGATGAAAGATATGGACAGTGTGTCGGCCATGATGTCGCAGATCGGTCAGCGC
GCAAAAGAGGCAGCCGCGAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MAALTDADCLVVKIGSALLVDRDAGGLRQAWLASLAQDVAWLTRQGVKVVLVSSGSIALGRGILKLSDTDLPLEQAQAAA
AVGQIRLARAYEEVLAPHGITTGQVLMTLDDSSNRRRYLNSRATLKQLLSMGVVPIVNENDTIATDEIRFGDNDRLAAQI
AVTVGADQLVLLSDVDGFYSANPNIDPTAQRFDEIGEITPEIEDMAGDACSGLSKGGMKTKLMAAKIATAAGCRMAITEG
VVLNPLRALNEGAAATWFRADTDPQAARKGWIAAMKPKGTVHLDSGALAALRSGKSLLPAGVSHITGHFQRGDPVAIADG
AGHTVGHGLVRYTAEEAGRIMGRKSNEIESVLGYPARAALIHRDDMALTIQNGD

Sequences:

>Translated_374_residues
MAALTDADCLVVKIGSALLVDRDAGGLRQAWLASLAQDVAWLTRQGVKVVLVSSGSIALGRGILKLSDTDLPLEQAQAAA
AVGQIRLARAYEEVLAPHGITTGQVLMTLDDSSNRRRYLNSRATLKQLLSMGVVPIVNENDTIATDEIRFGDNDRLAAQI
AVTVGADQLVLLSDVDGFYSANPNIDPTAQRFDEIGEITPEIEDMAGDACSGLSKGGMKTKLMAAKIATAAGCRMAITEG
VVLNPLRALNEGAAATWFRADTDPQAARKGWIAAMKPKGTVHLDSGALAALRSGKSLLPAGVSHITGHFQRGDPVAIADG
AGHTVGHGLVRYTAEEAGRIMGRKSNEIESVLGYPARAALIHRDDMALTIQNGD
>Mature_373_residues
AALTDADCLVVKIGSALLVDRDAGGLRQAWLASLAQDVAWLTRQGVKVVLVSSGSIALGRGILKLSDTDLPLEQAQAAAA
VGQIRLARAYEEVLAPHGITTGQVLMTLDDSSNRRRYLNSRATLKQLLSMGVVPIVNENDTIATDEIRFGDNDRLAAQIA
VTVGADQLVLLSDVDGFYSANPNIDPTAQRFDEIGEITPEIEDMAGDACSGLSKGGMKTKLMAAKIATAAGCRMAITEGV
VLNPLRALNEGAAATWFRADTDPQAARKGWIAAMKPKGTVHLDSGALAALRSGKSLLPAGVSHITGHFQRGDPVAIADGA
GHTVGHGLVRYTAEEAGRIMGRKSNEIESVLGYPARAALIHRDDMALTIQNGD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=270, Percent_Identity=31.4814814814815, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI62912457, Length=268, Percent_Identity=31.7164179104478, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=40.2173913043478, Blast_Score=259, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17569969, Length=253, Percent_Identity=32.8063241106719, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=253, Percent_Identity=32.8063241106719, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=33.0917874396135, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=32.6241134751773, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21357643, Length=286, Percent_Identity=32.1678321678322, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ROSDO (Q165Y7)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682892.1
- ProteinModelPortal:   Q165Y7
- SMR:   Q165Y7
- STRING:   Q165Y7
- GeneID:   4196207
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2659
- NMPDR:   fig|375451.6.peg.2472
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q165Y7
- ProtClustDB:   PRK05429
- BioCyc:   RDEN375451:RD1_2659-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39335; Mature: 39204

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALTDADCLVVKIGSALLVDRDAGGLRQAWLASLAQDVAWLTRQGVKVVLVSSGSIALG
CCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEC
RGILKLSDTDLPLEQAQAAAAVGQIRLARAYEEVLAPHGITTGQVLMTLDDSSNRRRYLN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
SRATLKQLLSMGVVPIVNENDTIATDEIRFGDNDRLAAQIAVTVGADQLVLLSDVDGFYS
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCEEEEEEEEECCCCEEEEECCCCCCC
ANPNIDPTAQRFDEIGEITPEIEDMAGDACSGLSKGGMKTKLMAAKIATAAGCRMAITEG
CCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
VVLNPLRALNEGAAATWFRADTDPQAARKGWIAAMKPKGTVHLDSGALAALRSGKSLLPA
CHHCHHHHHCCCCCEEEEECCCCHHHHHCCCEEEECCCCEEEECCCHHHHHHCCCCCCCC
GVSHITGHFQRGDPVAIADGAGHTVGHGLVRYTAEEAGRIMGRKSNEIESVLGYPARAAL
CHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHEEE
IHRDDMALTIQNGD
EEECCEEEEEECCC
>Mature Secondary Structure 
AALTDADCLVVKIGSALLVDRDAGGLRQAWLASLAQDVAWLTRQGVKVVLVSSGSIALG
CCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEC
RGILKLSDTDLPLEQAQAAAAVGQIRLARAYEEVLAPHGITTGQVLMTLDDSSNRRRYLN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
SRATLKQLLSMGVVPIVNENDTIATDEIRFGDNDRLAAQIAVTVGADQLVLLSDVDGFYS
HHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCEEEEEEEEECCCCEEEEECCCCCCC
ANPNIDPTAQRFDEIGEITPEIEDMAGDACSGLSKGGMKTKLMAAKIATAAGCRMAITEG
CCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCC
VVLNPLRALNEGAAATWFRADTDPQAARKGWIAAMKPKGTVHLDSGALAALRSGKSLLPA
CHHCHHHHHCCCCCEEEEECCCCHHHHHCCCEEEECCCCEEEECCCHHHHHHCCCCCCCC
GVSHITGHFQRGDPVAIADGAGHTVGHGLVRYTAEEAGRIMGRKSNEIESVLGYPARAAL
CHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHEEE
IHRDDMALTIQNGD
EEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA