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The map label for this gene is proA

Identifier: 110679884

GI number: 110679884

Start: 2536464

End: 2537729

Strand: Reverse

Name: proA

Synonym: RD1_2658

Alternate gene names: 110679884

Gene position: 2537729-2536464 (Counterclockwise)

Preceding gene: 110679885

Following gene: 110679882

Centisome position: 61.4

GC content: 60.74

Gene sequence:

>1266_bases
ATGAAAGATATGGACAGTGTGTCGGCCATGATGTCGCAGATCGGTCAGCGCGCAAAAGAGGCAGCCGCGAGCTTGGGATT
TGCCTCGGTGGAACGTAAACACGCTGCCCTGATTTCTGCGGCAGAACACATCTGGACGCAGCGCGCCGCCATCCTTGAGG
CCAATGCAAAAGACATCGCTTATGGGCGCGACAAGGGGCTGAGCGATGCCATGATGGACCGGCTTTTGCTGGATGAAGAG
CGCTTGCGGGGGATCGTCACCAGCCTGCGCTCCGTGGCGGAACAGGCGGATCCGGTCGGCGAAGTGATCGCTGCCTGGGA
TCAGCCGACGGGCCTGCATATTGAACGGGTGCGCACGCCGCTTGGTGTTATCGGCGTGATTTACGAGAGCCGCCCCAATG
TCACGGTGGATGCGGGCGCGCTGTGCCTGAAGGCGGGCAATGCGGTCATTCTGCGCGGCGGTTCGGAGAGTTTTCATTCC
TCCAAAGCGCTCCATGCCTGTCTGGTTCAGGGCCTCCGGGATGCCAATCTGCCAGAGGCCGCGATCCAGCTTGTGCCCAC
CCGGGATCGCGCGGCGGTCAGCGAAATGCTGACGATGACCGATACAATTGATGTAATCGTGCCGCGCGGGGGCAAGGGGC
TGGTCGGTCTTGTGCAGCGCGAGGCACGCGTTCCTGTCTTCGCGCATCTTGAGGGGATCGTGCACATCTATATCGACGCG
GCAGCGGATGCTGAAAAAACGCTCAAAGTGGTGATGAATGCCAAGACGCGGCGCACCGGTATCTGCGGGGCGGCTGAATG
CCTGCTGATCCACAAGGATGTGATGCCTACACTGGGCGCGGATGTCATAAAAGAGTTGATGCGCGCGGGTGTTGAGGTGC
GGGGGGACGAAACCCTGTCCGCGATCCAAGGGGTGGTCAAGGCCACGGCCGATGATTGGGGGCGGGAGTACCTTGATATG
ATCGTCGCGGCCCGCGTCGTTGATGATATTGACGATGCGATGGCGCATATCCGGACCCATGGGTCGAACCACACCGACTG
CATCCTGACGGAAGACGCCCGTGCTGCGCGGCGGTTTATGACGGAATTGGATTCAGCCATCGTCATGCATAATGCCTCGA
CGCAATTTGCAGATGGGGGGGAGTTCGGGATGGGTGCCGAAATTGGGATTGCGACCGGCAAGATGCATGCGCGCGGACCG
GTGGGGGCCGCGCAATTGACCAGTTTCAAGTATCTGGTGAGCGGTGACGGGACAACGCGTGCCTGA

Upstream 100 bases:

>100_bases
TTGGCTATCCCGCGCGTGCGGCTTTGATTCACCGGGACGATATGGCATTAACGATCCAGAATGGCGATTGATTTCGAAGA
GGGCGTGAGGGGGTAAGCAG

Downstream 100 bases:

>100_bases
AGCGTCTGATCAGATACTTGATGTATCAATCATCGCAGTATGCGTTGATATCCATGATGTCAGGCCGCGTTAAGCGTTTC
ATGTATTTCGCGTGACGCTC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKDMDSVSAMMSQIGQRAKEAAASLGFASVERKHAALISAAEHIWTQRAAILEANAKDIAYGRDKGLSDAMMDRLLLDEE
RLRGIVTSLRSVAEQADPVGEVIAAWDQPTGLHIERVRTPLGVIGVIYESRPNVTVDAGALCLKAGNAVILRGGSESFHS
SKALHACLVQGLRDANLPEAAIQLVPTRDRAAVSEMLTMTDTIDVIVPRGGKGLVGLVQREARVPVFAHLEGIVHIYIDA
AADAEKTLKVVMNAKTRRTGICGAAECLLIHKDVMPTLGADVIKELMRAGVEVRGDETLSAIQGVVKATADDWGREYLDM
IVAARVVDDIDDAMAHIRTHGSNHTDCILTEDARAARRFMTELDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGAAQLTSFKYLVSGDGTTRA

Sequences:

>Translated_421_residues
MKDMDSVSAMMSQIGQRAKEAAASLGFASVERKHAALISAAEHIWTQRAAILEANAKDIAYGRDKGLSDAMMDRLLLDEE
RLRGIVTSLRSVAEQADPVGEVIAAWDQPTGLHIERVRTPLGVIGVIYESRPNVTVDAGALCLKAGNAVILRGGSESFHS
SKALHACLVQGLRDANLPEAAIQLVPTRDRAAVSEMLTMTDTIDVIVPRGGKGLVGLVQREARVPVFAHLEGIVHIYIDA
AADAEKTLKVVMNAKTRRTGICGAAECLLIHKDVMPTLGADVIKELMRAGVEVRGDETLSAIQGVVKATADDWGREYLDM
IVAARVVDDIDDAMAHIRTHGSNHTDCILTEDARAARRFMTELDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGAAQLTSFKYLVSGDGTTRA
>Mature_421_residues
MKDMDSVSAMMSQIGQRAKEAAASLGFASVERKHAALISAAEHIWTQRAAILEANAKDIAYGRDKGLSDAMMDRLLLDEE
RLRGIVTSLRSVAEQADPVGEVIAAWDQPTGLHIERVRTPLGVIGVIYESRPNVTVDAGALCLKAGNAVILRGGSESFHS
SKALHACLVQGLRDANLPEAAIQLVPTRDRAAVSEMLTMTDTIDVIVPRGGKGLVGLVQREARVPVFAHLEGIVHIYIDA
AADAEKTLKVVMNAKTRRTGICGAAECLLIHKDVMPTLGADVIKELMRAGVEVRGDETLSAIQGVVKATADDWGREYLDM
IVAARVVDDIDDAMAHIRTHGSNHTDCILTEDARAARRFMTELDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGP
VGAAQLTSFKYLVSGDGTTRA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=410, Percent_Identity=36.3414634146341, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI21361368, Length=410, Percent_Identity=36.3414634146341, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=44.8441247002398, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17569969, Length=411, Percent_Identity=35.5231143552311, Blast_Score=267, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17569967, Length=411, Percent_Identity=35.5231143552311, Blast_Score=267, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=35.8139534883721, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI21357643, Length=387, Percent_Identity=38.2428940568475, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_ROSDO (Q165Y8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682891.1
- ProteinModelPortal:   Q165Y8
- SMR:   Q165Y8
- STRING:   Q165Y8
- GeneID:   4196206
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2658
- NMPDR:   fig|375451.6.peg.2471
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- PhylomeDB:   Q165Y8
- ProtClustDB:   PRK00197
- BioCyc:   RDEN375451:RD1_2658-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44950; Mature: 44950

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDMDSVSAMMSQIGQRAKEAAASLGFASVERKHAALISAAEHIWTQRAAILEANAKDIA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
YGRDKGLSDAMMDRLLLDEERLRGIVTSLRSVAEQADPVGEVIAAWDQPTGLHIERVRTP
CCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCC
LGVIGVIYESRPNVTVDAGALCLKAGNAVILRGGSESFHSSKALHACLVQGLRDANLPEA
HHEEEEEEECCCCEEEECCHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHH
AIQLVPTRDRAAVSEMLTMTDTIDVIVPRGGKGLVGLVQREARVPVFAHLEGIVHIYIDA
HHEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCHHHHHCCEEEEEEEC
AADAEKTLKVVMNAKTRRTGICGAAECLLIHKDVMPTLGADVIKELMRAGVEVRGDETLS
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCHHHH
AIQGVVKATADDWGREYLDMIVAARVVDDIDDAMAHIRTHGSNHTDCILTEDARAARRFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
TELDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGPVGAAQLTSFKYLVSGDGTTR
HHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHEEEEEECCCCCC
A
C
>Mature Secondary Structure
MKDMDSVSAMMSQIGQRAKEAAASLGFASVERKHAALISAAEHIWTQRAAILEANAKDIA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
YGRDKGLSDAMMDRLLLDEERLRGIVTSLRSVAEQADPVGEVIAAWDQPTGLHIERVRTP
CCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCC
LGVIGVIYESRPNVTVDAGALCLKAGNAVILRGGSESFHSSKALHACLVQGLRDANLPEA
HHEEEEEEECCCCEEEECCHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHH
AIQLVPTRDRAAVSEMLTMTDTIDVIVPRGGKGLVGLVQREARVPVFAHLEGIVHIYIDA
HHEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCHHHHHCCEEEEEEEC
AADAEKTLKVVMNAKTRRTGICGAAECLLIHKDVMPTLGADVIKELMRAGVEVRGDETLS
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCHHHH
AIQGVVKATADDWGREYLDMIVAARVVDDIDDAMAHIRTHGSNHTDCILTEDARAARRFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
TELDSAIVMHNASTQFADGGEFGMGAEIGIATGKMHARGPVGAAQLTSFKYLVSGDGTTR
HHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHEEEEEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA